Hepatoma-Targeted Radionuclide Immune Albumin Nanospheres: 131I-antiAFPMcAb-GCV-BSA-NPs
An effective strategy has been developed for synthesis of radionuclide immune albumin nanospheres (131I-antiAFPMcAb-GCV-BSA-NPs). In vitro as well as in vivo targeting of 131I-antiAFPMcAb-GCV-BSA-NPs to AFP-positive hepatoma was examined. In cultured HepG2 cells, the uptake and retention rates of 13...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2016/9142198 |
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| author | Mei Lin Junxing Huang Dongsheng Zhang Xingmao Jiang Jia Zhang Hong Yu Yanhong Xiao Yujuan Shi Ting Guo |
| author_facet | Mei Lin Junxing Huang Dongsheng Zhang Xingmao Jiang Jia Zhang Hong Yu Yanhong Xiao Yujuan Shi Ting Guo |
| author_sort | Mei Lin |
| collection | DOAJ |
| description | An effective strategy has been developed for synthesis of radionuclide immune albumin nanospheres (131I-antiAFPMcAb-GCV-BSA-NPs). In vitro as well as in vivo targeting of 131I-antiAFPMcAb-GCV-BSA-NPs to AFP-positive hepatoma was examined. In cultured HepG2 cells, the uptake and retention rates of 131I-antiAFPMcAb-GCV-BSA-NPs were remarkably higher than those of 131I alone. As well, the uptake rate and retention ratios of 131I-antiAFPMcAb-GCV-BSA-NPs in AFP-positive HepG2 cells were also significantly higher than those in AFP-negative HEK293 cells. Compared to 131I alone, 131I-antiAFPMcAb-GCV-BSA-NPs were much more easily taken in and retained by hepatoma tissue, with a much higher T/NT. Due to good drug-loading, high encapsulation ratio, and highly selective affinity for AFP-positive tumors, the 131I-antiAFPMcAb-GCV-BSA-NPs are promising for further effective radiation-gene therapy of hepatoma. |
| format | Article |
| id | doaj-art-747a4df4ca94476986b3b904a0761b86 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-747a4df4ca94476986b3b904a0761b862025-02-03T01:28:09ZengWileyAnalytical Cellular Pathology2210-71772210-71852016-01-01201610.1155/2016/91421989142198Hepatoma-Targeted Radionuclide Immune Albumin Nanospheres: 131I-antiAFPMcAb-GCV-BSA-NPsMei Lin0Junxing Huang1Dongsheng Zhang2Xingmao Jiang3Jia Zhang4Hong Yu5Yanhong Xiao6Yujuan Shi7Ting Guo8Clinical Medical Institute, Taizhou People’s Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, ChinaClinical Medical Institute, Taizhou People’s Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, ChinaMedical School, Southeast University, Nanjing, Jiangsu 210009, ChinaKey Laboratory of Advanced Catalytic Material and Technology, Changzhou University, Changzhou, Jiangsu 213000, ChinaMedical School, Southeast University, Nanjing, Jiangsu 210009, ChinaClinical Medical Institute, Taizhou People’s Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, ChinaClinical Medical Institute, Taizhou People’s Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, ChinaClinical Medical Institute, Taizhou People’s Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, ChinaClinical Medical Institute, Taizhou People’s Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, ChinaAn effective strategy has been developed for synthesis of radionuclide immune albumin nanospheres (131I-antiAFPMcAb-GCV-BSA-NPs). In vitro as well as in vivo targeting of 131I-antiAFPMcAb-GCV-BSA-NPs to AFP-positive hepatoma was examined. In cultured HepG2 cells, the uptake and retention rates of 131I-antiAFPMcAb-GCV-BSA-NPs were remarkably higher than those of 131I alone. As well, the uptake rate and retention ratios of 131I-antiAFPMcAb-GCV-BSA-NPs in AFP-positive HepG2 cells were also significantly higher than those in AFP-negative HEK293 cells. Compared to 131I alone, 131I-antiAFPMcAb-GCV-BSA-NPs were much more easily taken in and retained by hepatoma tissue, with a much higher T/NT. Due to good drug-loading, high encapsulation ratio, and highly selective affinity for AFP-positive tumors, the 131I-antiAFPMcAb-GCV-BSA-NPs are promising for further effective radiation-gene therapy of hepatoma.http://dx.doi.org/10.1155/2016/9142198 |
| spellingShingle | Mei Lin Junxing Huang Dongsheng Zhang Xingmao Jiang Jia Zhang Hong Yu Yanhong Xiao Yujuan Shi Ting Guo Hepatoma-Targeted Radionuclide Immune Albumin Nanospheres: 131I-antiAFPMcAb-GCV-BSA-NPs Analytical Cellular Pathology |
| title | Hepatoma-Targeted Radionuclide Immune Albumin Nanospheres: 131I-antiAFPMcAb-GCV-BSA-NPs |
| title_full | Hepatoma-Targeted Radionuclide Immune Albumin Nanospheres: 131I-antiAFPMcAb-GCV-BSA-NPs |
| title_fullStr | Hepatoma-Targeted Radionuclide Immune Albumin Nanospheres: 131I-antiAFPMcAb-GCV-BSA-NPs |
| title_full_unstemmed | Hepatoma-Targeted Radionuclide Immune Albumin Nanospheres: 131I-antiAFPMcAb-GCV-BSA-NPs |
| title_short | Hepatoma-Targeted Radionuclide Immune Albumin Nanospheres: 131I-antiAFPMcAb-GCV-BSA-NPs |
| title_sort | hepatoma targeted radionuclide immune albumin nanospheres 131i antiafpmcab gcv bsa nps |
| url | http://dx.doi.org/10.1155/2016/9142198 |
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