Role of lysosomal enzymes released by alveolar macrophages in the pathogenesis of the acute phase of hypersensitivity pneumonitis
Hydrolytic enzymes are the major constituents of alveolar macrophages (AM) and have been shown to be involved in many aspects of the inflammatory pulmonary response. The aim of this study was to evaluate the role of lysosomal enzymes in the acute phase of hypersensitivity pneumonitis (HPs). An exper...
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| Format: | Article |
| Language: | English |
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Wiley
1995-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935195000081 |
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| author | J. L. Pérez-Arellano M. N. Barrios T. Martín M. L. Sánchez J. M. González Buitrago A. Jiménez |
| author_facet | J. L. Pérez-Arellano M. N. Barrios T. Martín M. L. Sánchez J. M. González Buitrago A. Jiménez |
| author_sort | J. L. Pérez-Arellano |
| collection | DOAJ |
| description | Hydrolytic enzymes are the major constituents of alveolar macrophages (AM) and have been shown to be involved in many aspects of the inflammatory pulmonary response. The aim of this study was to evaluate the role of lysosomal enzymes in the acute phase of hypersensitivity pneumonitis (HPs). An experimental study on AM lysosomal enzymes of an HP-guinea-pig model was performed. The results obtained both in vivo and in vitro suggest that intracellular enzymatic activity decrease is, at least partly, due to release of lysosomal enzymes into the medium. A positive but slight correlation was found between extracellular lysosomal activity and four parameters of lung lesion (lung index, bronchoalveolar fluid total (BALF) protein concentration, BALF LDH and BALF alkaline phosphatase activities). All the above findings suggest that the AM release of lysosomal enzymes during HP is a factor involved, although possibly not the only one, in the pulmonary lesions appearing in this disease. |
| format | Article |
| id | doaj-art-744ef34fecb847ba97cd570e90e11ac5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-744ef34fecb847ba97cd570e90e11ac52025-02-03T05:51:24ZengWileyMediators of Inflammation0962-93511466-18611995-01-0141434810.1155/S0962935195000081Role of lysosomal enzymes released by alveolar macrophages in the pathogenesis of the acute phase of hypersensitivity pneumonitisJ. L. Pérez-Arellano0M. N. Barrios1T. Martín2M. L. Sánchez3J. M. González Buitrago4A. Jiménez5Departamento de Medicina, Facultad de Medicina, Universidad de Salamanca, Avda Campo Charro s/n, Salamanca 37007, SpainDepartamento de Medicina, Facultad de Medicina, Universidad de Salamanca, Avda Campo Charro s/n, Salamanca 37007, SpainDepartamento de Medicina, Facultad de Medicina, Universidad de Salamanca, Avda Campo Charro s/n, Salamanca 37007, SpainDepartamento de Medicina, Facultad de Medicina, Universidad de Salamanca, Avda Campo Charro s/n, Salamanca 37007, SpainServicio de Bioquimica, Hospital Virgen de la Vega, Paseo de San Vincente s/n, Salamanca 37000, SpainDepartamento de Medicina, Facultad de Medicina, Universidad de Salamanca, Avda Campo Charro s/n, Salamanca 37007, SpainHydrolytic enzymes are the major constituents of alveolar macrophages (AM) and have been shown to be involved in many aspects of the inflammatory pulmonary response. The aim of this study was to evaluate the role of lysosomal enzymes in the acute phase of hypersensitivity pneumonitis (HPs). An experimental study on AM lysosomal enzymes of an HP-guinea-pig model was performed. The results obtained both in vivo and in vitro suggest that intracellular enzymatic activity decrease is, at least partly, due to release of lysosomal enzymes into the medium. A positive but slight correlation was found between extracellular lysosomal activity and four parameters of lung lesion (lung index, bronchoalveolar fluid total (BALF) protein concentration, BALF LDH and BALF alkaline phosphatase activities). All the above findings suggest that the AM release of lysosomal enzymes during HP is a factor involved, although possibly not the only one, in the pulmonary lesions appearing in this disease.http://dx.doi.org/10.1155/S0962935195000081 |
| spellingShingle | J. L. Pérez-Arellano M. N. Barrios T. Martín M. L. Sánchez J. M. González Buitrago A. Jiménez Role of lysosomal enzymes released by alveolar macrophages in the pathogenesis of the acute phase of hypersensitivity pneumonitis Mediators of Inflammation |
| title | Role of lysosomal enzymes released by alveolar macrophages in the pathogenesis of the acute phase of hypersensitivity pneumonitis |
| title_full | Role of lysosomal enzymes released by alveolar macrophages in the pathogenesis of the acute phase of hypersensitivity pneumonitis |
| title_fullStr | Role of lysosomal enzymes released by alveolar macrophages in the pathogenesis of the acute phase of hypersensitivity pneumonitis |
| title_full_unstemmed | Role of lysosomal enzymes released by alveolar macrophages in the pathogenesis of the acute phase of hypersensitivity pneumonitis |
| title_short | Role of lysosomal enzymes released by alveolar macrophages in the pathogenesis of the acute phase of hypersensitivity pneumonitis |
| title_sort | role of lysosomal enzymes released by alveolar macrophages in the pathogenesis of the acute phase of hypersensitivity pneumonitis |
| url | http://dx.doi.org/10.1155/S0962935195000081 |
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