Blocking Interleukin-33 Alleviates the Joint Inflammation and Inhibits the Development of Collagen-Induced Arthritis in Mice
Rheumatoid arthritis (RA) is considered a systemic chronic inflammatory joint disease characterized by chronic synovitis and cartilage and bone destruction. Interleukin-33 (IL-33) is a proinflammatory cytokine which is highly expressed in the synovium of RA patients and the joints of mice with colla...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/4297354 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554019398090752 |
|---|---|
| author | Yan Li Yeqin Fu Huan Chen Xiaojin Liu Mingcai Li |
| author_facet | Yan Li Yeqin Fu Huan Chen Xiaojin Liu Mingcai Li |
| author_sort | Yan Li |
| collection | DOAJ |
| description | Rheumatoid arthritis (RA) is considered a systemic chronic inflammatory joint disease characterized by chronic synovitis and cartilage and bone destruction. Interleukin-33 (IL-33) is a proinflammatory cytokine which is highly expressed in the synovium of RA patients and the joints of mice with collagen-induced arthritis (CIA) and exacerbates CIA in mice. However, the role of the IL-33-neutralizing antibody in the murine model of CIA remains unclear. In the present study, CIA mice were given intraperitoneally with polyclonal rabbit anti-murine IL-33 antibody (anti-IL-33) or normal rabbit IgG control after the first signs of arthritis. Administration of anti-IL-33 after the onset of disease significantly reduced the severity of CIA and joint damage compared with controls treated with normal rabbit IgG. Anti-IL-33 treatment also significantly decreased the serum levels of interferon-γ(IFN-γ),IL-6, IL-12, IL-33, and tumor necrosis factor-α (TNF-α). Moreover, anti-IL-33 treatment significantly downregulated the production of IFN-γ, IL-6, IL-12, IL-33, and TNF-α in ex vivo-stimulated spleen cells. Together, our results indicate that the IL-33-neutralizing antibody may provide a therapeutic strategy for RA by inhibiting the release of proinflammatory cytokines. |
| format | Article |
| id | doaj-art-7420464f2b06403d8d7763750defb6a4 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-7420464f2b06403d8d7763750defb6a42025-02-03T05:52:34ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/42973544297354Blocking Interleukin-33 Alleviates the Joint Inflammation and Inhibits the Development of Collagen-Induced Arthritis in MiceYan Li0Yeqin Fu1Huan Chen2Xiaojin Liu3Mingcai Li4Department of Immunology, Ningbo University School of Medicine, Ningbo, ChinaDepartment of Immunology, Ningbo University School of Medicine, Ningbo, ChinaDepartment of Immunology, Ningbo University School of Medicine, Ningbo, ChinaDepartment of Clinical Laboratory, The Fifth Hospital of Shijiazhuang, Shijiazhuang, ChinaDepartment of Immunology, Ningbo University School of Medicine, Ningbo, ChinaRheumatoid arthritis (RA) is considered a systemic chronic inflammatory joint disease characterized by chronic synovitis and cartilage and bone destruction. Interleukin-33 (IL-33) is a proinflammatory cytokine which is highly expressed in the synovium of RA patients and the joints of mice with collagen-induced arthritis (CIA) and exacerbates CIA in mice. However, the role of the IL-33-neutralizing antibody in the murine model of CIA remains unclear. In the present study, CIA mice were given intraperitoneally with polyclonal rabbit anti-murine IL-33 antibody (anti-IL-33) or normal rabbit IgG control after the first signs of arthritis. Administration of anti-IL-33 after the onset of disease significantly reduced the severity of CIA and joint damage compared with controls treated with normal rabbit IgG. Anti-IL-33 treatment also significantly decreased the serum levels of interferon-γ(IFN-γ),IL-6, IL-12, IL-33, and tumor necrosis factor-α (TNF-α). Moreover, anti-IL-33 treatment significantly downregulated the production of IFN-γ, IL-6, IL-12, IL-33, and TNF-α in ex vivo-stimulated spleen cells. Together, our results indicate that the IL-33-neutralizing antibody may provide a therapeutic strategy for RA by inhibiting the release of proinflammatory cytokines.http://dx.doi.org/10.1155/2020/4297354 |
| spellingShingle | Yan Li Yeqin Fu Huan Chen Xiaojin Liu Mingcai Li Blocking Interleukin-33 Alleviates the Joint Inflammation and Inhibits the Development of Collagen-Induced Arthritis in Mice Journal of Immunology Research |
| title | Blocking Interleukin-33 Alleviates the Joint Inflammation and Inhibits the Development of Collagen-Induced Arthritis in Mice |
| title_full | Blocking Interleukin-33 Alleviates the Joint Inflammation and Inhibits the Development of Collagen-Induced Arthritis in Mice |
| title_fullStr | Blocking Interleukin-33 Alleviates the Joint Inflammation and Inhibits the Development of Collagen-Induced Arthritis in Mice |
| title_full_unstemmed | Blocking Interleukin-33 Alleviates the Joint Inflammation and Inhibits the Development of Collagen-Induced Arthritis in Mice |
| title_short | Blocking Interleukin-33 Alleviates the Joint Inflammation and Inhibits the Development of Collagen-Induced Arthritis in Mice |
| title_sort | blocking interleukin 33 alleviates the joint inflammation and inhibits the development of collagen induced arthritis in mice |
| url | http://dx.doi.org/10.1155/2020/4297354 |
| work_keys_str_mv | AT yanli blockinginterleukin33alleviatesthejointinflammationandinhibitsthedevelopmentofcollageninducedarthritisinmice AT yeqinfu blockinginterleukin33alleviatesthejointinflammationandinhibitsthedevelopmentofcollageninducedarthritisinmice AT huanchen blockinginterleukin33alleviatesthejointinflammationandinhibitsthedevelopmentofcollageninducedarthritisinmice AT xiaojinliu blockinginterleukin33alleviatesthejointinflammationandinhibitsthedevelopmentofcollageninducedarthritisinmice AT mingcaili blockinginterleukin33alleviatesthejointinflammationandinhibitsthedevelopmentofcollageninducedarthritisinmice |