Advanced Methods in Fluorescence Microscopy
It requires a good deal of will power to resist hyperbole in considering the advances that have been achieved in fluorescence microscopy in the last 25 years. Our effort has been to survey the modalities of microscopic fluorescence imaging available to cell biologists and perhaps useful for diagnost...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.3233/ACP-120071 |
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| _version_ | 1832565391717564416 |
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| author | Luke Fritzky David Lagunoff |
| author_facet | Luke Fritzky David Lagunoff |
| author_sort | Luke Fritzky |
| collection | DOAJ |
| description | It requires a good deal of will power to resist hyperbole in considering the advances that have been achieved in fluorescence microscopy in the last 25 years. Our effort has been to survey the modalities of microscopic fluorescence imaging available to cell biologists and perhaps useful for diagnostic pathologists. The gamut extends from established confocal laser scanning through multiphoton and TIRF to the emerging technologies of super-resolution microscopy that breech the Abbé limit of resolution. Also considered are the recent innovations in structured and light sheet illumination, the use of FRET and molecular beacons that exploit specific characteristics of designer fluorescent proteins, fluorescence speckles, and second harmonic generation for native anisometric structures like collagen, microtubules and sarcomeres. |
| format | Article |
| id | doaj-art-74146e9dd0834a5da7a590e3472ab5e8 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-74146e9dd0834a5da7a590e3472ab5e82025-02-03T01:07:58ZengWileyAnalytical Cellular Pathology2210-71772210-71852013-01-01361-251710.3233/ACP-120071Advanced Methods in Fluorescence MicroscopyLuke Fritzky0David Lagunoff1Core Imaging Facility, New Jersey Medical School, UM, NJ, USACore Imaging Facility, New Jersey Medical School, UM, NJ, USAIt requires a good deal of will power to resist hyperbole in considering the advances that have been achieved in fluorescence microscopy in the last 25 years. Our effort has been to survey the modalities of microscopic fluorescence imaging available to cell biologists and perhaps useful for diagnostic pathologists. The gamut extends from established confocal laser scanning through multiphoton and TIRF to the emerging technologies of super-resolution microscopy that breech the Abbé limit of resolution. Also considered are the recent innovations in structured and light sheet illumination, the use of FRET and molecular beacons that exploit specific characteristics of designer fluorescent proteins, fluorescence speckles, and second harmonic generation for native anisometric structures like collagen, microtubules and sarcomeres.http://dx.doi.org/10.3233/ACP-120071 |
| spellingShingle | Luke Fritzky David Lagunoff Advanced Methods in Fluorescence Microscopy Analytical Cellular Pathology |
| title | Advanced Methods in Fluorescence Microscopy |
| title_full | Advanced Methods in Fluorescence Microscopy |
| title_fullStr | Advanced Methods in Fluorescence Microscopy |
| title_full_unstemmed | Advanced Methods in Fluorescence Microscopy |
| title_short | Advanced Methods in Fluorescence Microscopy |
| title_sort | advanced methods in fluorescence microscopy |
| url | http://dx.doi.org/10.3233/ACP-120071 |
| work_keys_str_mv | AT lukefritzky advancedmethodsinfluorescencemicroscopy AT davidlagunoff advancedmethodsinfluorescencemicroscopy |