Genotypic Investigation of Clostridium difficile in Prince Edward Island
Clostridium difficile is an important cause of disease in Canada; however, little information is available about the disease in the Maritime provinces. The objective of the present study was to characterize C difficile isolates obtained from people hospitalized with C difficile infection in Prince E...
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| Format: | Article |
| Language: | English |
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Wiley
2008-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2008/848045 |
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| author | H Martin LP Abbott DE Low B Willey M Mulvey J Scott Weese |
| author_facet | H Martin LP Abbott DE Low B Willey M Mulvey J Scott Weese |
| author_sort | H Martin |
| collection | DOAJ |
| description | Clostridium difficile is an important cause of disease in Canada; however, little information is available about the disease in the Maritime provinces. The objective of the present study was to characterize C difficile isolates obtained from people hospitalized with C difficile infection in Prince Edward Island. One hundred twenty-six C difficile ELISA toxin-positive stool samples were obtained and cultured using an enrichment protocol. C difficile was isolated from 105 of 126 (83%) samples. Twenty-two different ribotypes were identified. The most common ribotype, ribotype W, was a North American pulsotype 2 (NAP2), toxinotype 0 strain, which represented 18% of isolates. The next most common ribotype was a NAP1, toxinotype III strain, which accounted for 11% of isolates. Ribotype 027/NAP1 only accounted for five (4.7%) isolates. Forty-five per cent of isolates possessed genes encoding production of binary toxin. Three different ribotypes, all NAP1, toxinotype III strains, had a frameshift mutation in the tcdC gene (Δ117), while one isolate (ribotype 078, NAP4, toxinotype V) had a truncating mutation (C184T) in the tcdC gene. |
| format | Article |
| id | doaj-art-73bed216b221405b8f90eef5c5ba341d |
| institution | Kabale University |
| issn | 1712-9532 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-73bed216b221405b8f90eef5c5ba341d2025-02-03T05:49:48ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95322008-01-0119640941210.1155/2008/848045Genotypic Investigation of Clostridium difficile in Prince Edward IslandH Martin0LP Abbott1DE Low2B Willey3M Mulvey4J Scott Weese5Department of Clinical Studies, University of Guelph, Guelph, Ontario, CanadaQueen Elizabeth Hospital, Charlottetown, Prince Edward Island, CanadaDepartment of Microbiology, Mount Sinai Hospital, Toronto, Ontario, CanadaDepartment of Microbiology, Mount Sinai Hospital, Toronto, Ontario, CanadaNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, CanadaDepartment of Pathobiology, University of Guelph, Guelph, Ontario, CanadaClostridium difficile is an important cause of disease in Canada; however, little information is available about the disease in the Maritime provinces. The objective of the present study was to characterize C difficile isolates obtained from people hospitalized with C difficile infection in Prince Edward Island. One hundred twenty-six C difficile ELISA toxin-positive stool samples were obtained and cultured using an enrichment protocol. C difficile was isolated from 105 of 126 (83%) samples. Twenty-two different ribotypes were identified. The most common ribotype, ribotype W, was a North American pulsotype 2 (NAP2), toxinotype 0 strain, which represented 18% of isolates. The next most common ribotype was a NAP1, toxinotype III strain, which accounted for 11% of isolates. Ribotype 027/NAP1 only accounted for five (4.7%) isolates. Forty-five per cent of isolates possessed genes encoding production of binary toxin. Three different ribotypes, all NAP1, toxinotype III strains, had a frameshift mutation in the tcdC gene (Δ117), while one isolate (ribotype 078, NAP4, toxinotype V) had a truncating mutation (C184T) in the tcdC gene.http://dx.doi.org/10.1155/2008/848045 |
| spellingShingle | H Martin LP Abbott DE Low B Willey M Mulvey J Scott Weese Genotypic Investigation of Clostridium difficile in Prince Edward Island Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Genotypic Investigation of Clostridium difficile in Prince Edward Island |
| title_full | Genotypic Investigation of Clostridium difficile in Prince Edward Island |
| title_fullStr | Genotypic Investigation of Clostridium difficile in Prince Edward Island |
| title_full_unstemmed | Genotypic Investigation of Clostridium difficile in Prince Edward Island |
| title_short | Genotypic Investigation of Clostridium difficile in Prince Edward Island |
| title_sort | genotypic investigation of clostridium difficile in prince edward island |
| url | http://dx.doi.org/10.1155/2008/848045 |
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