STUDY ON IN VITRO PROPAGATION AND CULTIVATION OF ANOECTOCHILUS LYLEI ROLFE EX DOWNIES AT EX VITRO CONDITION
In the present study, we investigated in vitro propagation and cultivated Anoectochilus lylei Rolfe ex Downies at ex vitro condition, which is one of the valuable herbal plants and has been proved to be good for human health. The results showed that, MS medium supplemented with 1 mg/l BA gave the be...
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Dalat University
2017-01-01
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| Series: | Tạp chí Khoa học Đại học Đà Lạt |
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| Online Access: | http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/90 |
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| author | Phan Xuân Huyên Nguyễn Văn Kết Phan Hoàng Đại Nguyễn Thị Cúc |
| author_facet | Phan Xuân Huyên Nguyễn Văn Kết Phan Hoàng Đại Nguyễn Thị Cúc |
| author_sort | Phan Xuân Huyên |
| collection | DOAJ |
| description | In the present study, we investigated in vitro propagation and cultivated Anoectochilus lylei Rolfe ex Downies at ex vitro condition, which is one of the valuable herbal plants and has been proved to be good for human health. The results showed that, MS medium supplemented with 1 mg/l BA gave the best on in vitro shoot regeneration after 60 days of culture, with 5.70 shoots/explant, shoot height of 3.72 cm. MS medium containing 0 – 2 mg/l NAA was found to be the most suitable for in vitro root regeneration after 30 days of culture, root regeneration rate of 100%. Substrate of 90% coconut fiber powder combination with 10% rice husk was the best substrate for acclimatization of the plantlet after 60 days of cultivation, with plant height of 7.00 cm, root length of 4.74 cm, survival rate of 100%. For the cultivation of Anoectochilus lylei, after 120 days of cultivation, the result showed that, the plants which were used with Nitrophoska® Foliar of 2 g/l (plant height of 11.00 cm, root length of 7.60 cm, fresh weight of 1.81 g/plant, survival rate of 100%) were better than Nitrophoska® Foliar of 1 g/l (plant height of 9.80 cm, root length of 6.70 cm, fresh weight of 1.64 g/plant, survival rate of 100%). |
| format | Article |
| id | doaj-art-73413c03d7084eccb39d2b8944e51354 |
| institution | Kabale University |
| issn | 0866-787X 0866-787X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Dalat University |
| record_format | Article |
| series | Tạp chí Khoa học Đại học Đà Lạt |
| spelling | doaj-art-73413c03d7084eccb39d2b8944e513542025-02-02T17:13:48ZengDalat UniversityTạp chí Khoa học Đại học Đà Lạt0866-787X0866-787X2017-01-016448149210.37569/DalatUniversity.6.4.90(2016)46STUDY ON IN VITRO PROPAGATION AND CULTIVATION OF ANOECTOCHILUS LYLEI ROLFE EX DOWNIES AT EX VITRO CONDITIONPhan Xuân Huyên0Nguyễn Văn Kết1Phan Hoàng Đại2Nguyễn Thị Cúc3Viện Nghiên cứu Khoa học Tây NguyênKhoa Nông Lâm, Trường Đại học Đà LạtViện Nghiên cứu và Ứng dụng Nông nghiệp Công nghệ cao, Trường Đại học Đà LạtKhoa Nông Lâm, Trường Đại học Đà LạtIn the present study, we investigated in vitro propagation and cultivated Anoectochilus lylei Rolfe ex Downies at ex vitro condition, which is one of the valuable herbal plants and has been proved to be good for human health. The results showed that, MS medium supplemented with 1 mg/l BA gave the best on in vitro shoot regeneration after 60 days of culture, with 5.70 shoots/explant, shoot height of 3.72 cm. MS medium containing 0 – 2 mg/l NAA was found to be the most suitable for in vitro root regeneration after 30 days of culture, root regeneration rate of 100%. Substrate of 90% coconut fiber powder combination with 10% rice husk was the best substrate for acclimatization of the plantlet after 60 days of cultivation, with plant height of 7.00 cm, root length of 4.74 cm, survival rate of 100%. For the cultivation of Anoectochilus lylei, after 120 days of cultivation, the result showed that, the plants which were used with Nitrophoska® Foliar of 2 g/l (plant height of 11.00 cm, root length of 7.60 cm, fresh weight of 1.81 g/plant, survival rate of 100%) were better than Nitrophoska® Foliar of 1 g/l (plant height of 9.80 cm, root length of 6.70 cm, fresh weight of 1.64 g/plant, survival rate of 100%).http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/90cây lan gấmgiá thểphân bónsự tái sinh chồisự tái sinh rễ |
| spellingShingle | Phan Xuân Huyên Nguyễn Văn Kết Phan Hoàng Đại Nguyễn Thị Cúc STUDY ON IN VITRO PROPAGATION AND CULTIVATION OF ANOECTOCHILUS LYLEI ROLFE EX DOWNIES AT EX VITRO CONDITION Tạp chí Khoa học Đại học Đà Lạt cây lan gấm giá thể phân bón sự tái sinh chồi sự tái sinh rễ |
| title | STUDY ON IN VITRO PROPAGATION AND CULTIVATION OF ANOECTOCHILUS LYLEI ROLFE EX DOWNIES AT EX VITRO CONDITION |
| title_full | STUDY ON IN VITRO PROPAGATION AND CULTIVATION OF ANOECTOCHILUS LYLEI ROLFE EX DOWNIES AT EX VITRO CONDITION |
| title_fullStr | STUDY ON IN VITRO PROPAGATION AND CULTIVATION OF ANOECTOCHILUS LYLEI ROLFE EX DOWNIES AT EX VITRO CONDITION |
| title_full_unstemmed | STUDY ON IN VITRO PROPAGATION AND CULTIVATION OF ANOECTOCHILUS LYLEI ROLFE EX DOWNIES AT EX VITRO CONDITION |
| title_short | STUDY ON IN VITRO PROPAGATION AND CULTIVATION OF ANOECTOCHILUS LYLEI ROLFE EX DOWNIES AT EX VITRO CONDITION |
| title_sort | study on in vitro propagation and cultivation of anoectochilus lylei rolfe ex downies at ex vitro condition |
| topic | cây lan gấm giá thể phân bón sự tái sinh chồi sự tái sinh rễ |
| url | http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/90 |
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