Development of a RP-HPLC Method for Separating and Quantifying Muscimol in Different Developmental Stages of the Fungus Amanita muscaria
A simple RP-HPLC method was designed for the quantification of muscimol (5-(aminomethyl)-isoxazol-3-ol) present in five aqueous extracts of Amanita muscaria each from a different developmental stage. Results show that the maximum concentration of muscimol (1,210 mg/ml) was found in the young mushroo...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/8859998 |
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| author | Emersón León Ávila James Guevara-Pulido |
| author_facet | Emersón León Ávila James Guevara-Pulido |
| author_sort | Emersón León Ávila |
| collection | DOAJ |
| description | A simple RP-HPLC method was designed for the quantification of muscimol (5-(aminomethyl)-isoxazol-3-ol) present in five aqueous extracts of Amanita muscaria each from a different developmental stage. Results show that the maximum concentration of muscimol (1,210 mg/ml) was found in the young mushroom stage of development. Moreover, it was also found that this concentration progressively decreases as the fungus ages. The developed method is a simple but effective method for the quantification of muscimol, a widely important metabolite for the pharmaceutical industry as a possible treatment for tardive dyskinesia and Parkinson’s disease. |
| format | Article |
| id | doaj-art-72f2663a56b54cef8719509bdf8720b9 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-72f2663a56b54cef8719509bdf8720b92025-02-03T01:00:11ZengWileyJournal of Chemistry2090-90632090-90712020-01-01202010.1155/2020/88599988859998Development of a RP-HPLC Method for Separating and Quantifying Muscimol in Different Developmental Stages of the Fungus Amanita muscariaEmersón León Ávila0James Guevara-Pulido1Universidad El Bosque, Química Farmacéutica, INQA Research Group, Av Cra 9 No. 131A-02, Bogotá 110121, ColombiaUniversidad El Bosque, Química Farmacéutica, INQA Research Group, Av Cra 9 No. 131A-02, Bogotá 110121, ColombiaA simple RP-HPLC method was designed for the quantification of muscimol (5-(aminomethyl)-isoxazol-3-ol) present in five aqueous extracts of Amanita muscaria each from a different developmental stage. Results show that the maximum concentration of muscimol (1,210 mg/ml) was found in the young mushroom stage of development. Moreover, it was also found that this concentration progressively decreases as the fungus ages. The developed method is a simple but effective method for the quantification of muscimol, a widely important metabolite for the pharmaceutical industry as a possible treatment for tardive dyskinesia and Parkinson’s disease.http://dx.doi.org/10.1155/2020/8859998 |
| spellingShingle | Emersón León Ávila James Guevara-Pulido Development of a RP-HPLC Method for Separating and Quantifying Muscimol in Different Developmental Stages of the Fungus Amanita muscaria Journal of Chemistry |
| title | Development of a RP-HPLC Method for Separating and Quantifying Muscimol in Different Developmental Stages of the Fungus Amanita muscaria |
| title_full | Development of a RP-HPLC Method for Separating and Quantifying Muscimol in Different Developmental Stages of the Fungus Amanita muscaria |
| title_fullStr | Development of a RP-HPLC Method for Separating and Quantifying Muscimol in Different Developmental Stages of the Fungus Amanita muscaria |
| title_full_unstemmed | Development of a RP-HPLC Method for Separating and Quantifying Muscimol in Different Developmental Stages of the Fungus Amanita muscaria |
| title_short | Development of a RP-HPLC Method for Separating and Quantifying Muscimol in Different Developmental Stages of the Fungus Amanita muscaria |
| title_sort | development of a rp hplc method for separating and quantifying muscimol in different developmental stages of the fungus amanita muscaria |
| url | http://dx.doi.org/10.1155/2020/8859998 |
| work_keys_str_mv | AT emersonleonavila developmentofarphplcmethodforseparatingandquantifyingmuscimolindifferentdevelopmentalstagesofthefungusamanitamuscaria AT jamesguevarapulido developmentofarphplcmethodforseparatingandquantifyingmuscimolindifferentdevelopmentalstagesofthefungusamanitamuscaria |