Interaction between PAF and human platelet membranes: a fluorescence study
The interaction between PAF and human platelet membranes was investigated by measuring the steadystate fluorescence anisotropy and fluorescence decay of 1 (4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in platelet plasma membranes. PAF induced a time-limited and signifi...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1992-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935192000218 |
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| _version_ | 1832564267556012032 |
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| author | Ahmad Kantar Pier Luigi Giorgi Giovanna Curatola Rosamaria Fiorini |
| author_facet | Ahmad Kantar Pier Luigi Giorgi Giovanna Curatola Rosamaria Fiorini |
| author_sort | Ahmad Kantar |
| collection | DOAJ |
| description | The interaction between PAF and human platelet membranes was investigated by measuring the steadystate fluorescence anisotropy and fluorescence decay of 1 (4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in platelet plasma membranes. PAF induced a time-limited and significant increase of the lipid order in the exterior part of the membrane and a decrease in membrane heterogeneity. These changes were blocked in the presence of the PAF antagonists, L-659,989 and 1-O-hexadecyl-2-acetyl-sn-glycero-3-phospho(N,N,N-trimethyl)hexanolamine.H2O. These results indicate that the observed changes in the physico-chemical properties of the membrane are attributed to the PAF–receptor interaction and signal transduction. |
| format | Article |
| id | doaj-art-72ed065f4a1641639f2222bee3d79100 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1992-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-72ed065f4a1641639f2222bee3d791002025-02-03T01:11:22ZengWileyMediators of Inflammation0962-93511466-18611992-01-011212713110.1155/S0962935192000218Interaction between PAF and human platelet membranes: a fluorescence studyAhmad Kantar0Pier Luigi Giorgi1Giovanna Curatola2Rosamaria Fiorini3Department of Pediatrics, University of Ancona, via Corridoni 11, Ancona l-60123, ItalyDepartment of Pediatrics, University of Ancona, via Corridoni 11, Ancona l-60123, ItalyDepartment of Biochemistry, University of Ancona, via Corridoni 11, Ancona l-60123, ItalyDepartment of Biochemistry, University of Ancona, via Corridoni 11, Ancona l-60123, ItalyThe interaction between PAF and human platelet membranes was investigated by measuring the steadystate fluorescence anisotropy and fluorescence decay of 1 (4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in platelet plasma membranes. PAF induced a time-limited and significant increase of the lipid order in the exterior part of the membrane and a decrease in membrane heterogeneity. These changes were blocked in the presence of the PAF antagonists, L-659,989 and 1-O-hexadecyl-2-acetyl-sn-glycero-3-phospho(N,N,N-trimethyl)hexanolamine.H2O. These results indicate that the observed changes in the physico-chemical properties of the membrane are attributed to the PAF–receptor interaction and signal transduction.http://dx.doi.org/10.1155/S0962935192000218 |
| spellingShingle | Ahmad Kantar Pier Luigi Giorgi Giovanna Curatola Rosamaria Fiorini Interaction between PAF and human platelet membranes: a fluorescence study Mediators of Inflammation |
| title | Interaction between PAF and human platelet membranes: a fluorescence study |
| title_full | Interaction between PAF and human platelet membranes: a fluorescence study |
| title_fullStr | Interaction between PAF and human platelet membranes: a fluorescence study |
| title_full_unstemmed | Interaction between PAF and human platelet membranes: a fluorescence study |
| title_short | Interaction between PAF and human platelet membranes: a fluorescence study |
| title_sort | interaction between paf and human platelet membranes a fluorescence study |
| url | http://dx.doi.org/10.1155/S0962935192000218 |
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