The effect of rotenone contamination on high-resolution mitochondrial respiration experiments

The effect of rotenone contamination on high-resolution mitochondrial respiration experiments

Background High-resolution respirometry is commonly used in skeletal muscle research and exercise science to measure mitochondrial respiratory function in both permeabilized muscle fibers and isolated mitochondria. Due to the low throughput and high cost of the most used respirometer, the Oroboros 2...

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Bibliographic Details
Main Authors: Dale F. Taylor, Jia Li, Nicholas J. Saner, Jia Wei, Xu Yan, Elizabeth G. Reisman, Hanzhe Li, Matthew J-C Lee, Navabeh Zare, Andrew Garnham, Jujiao Kuang, David J. Bishop
Format: Article
Language:English
Published: PeerJ Inc. 2025-08-01
Series:PeerJ
Subjects:
Oroboros O2k
Permeabilized fibers
Protocol development
Exercise
Exercise-induced adaptation
Respiration
Online Access:https://peerj.com/articles/19879.pdf
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