The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats
Background. The precise mechanisms of nerve regeneration remain unclear. The potential of facial nerve regeneration and probable mechanisms involved following chronic facial nerve injury should be further studied. Methods. Adult male Wistar rats were used to model either (i) facial nerve injury (axo...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2020/8884511 |
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| author | Yusu Ni Diyan Chen Yi Jiang Danhong Qiu Wen Li Huawei Li |
| author_facet | Yusu Ni Diyan Chen Yi Jiang Danhong Qiu Wen Li Huawei Li |
| author_sort | Yusu Ni |
| collection | DOAJ |
| description | Background. The precise mechanisms of nerve regeneration remain unclear. The potential of facial nerve regeneration and probable mechanisms involved following chronic facial nerve injury should be further studied. Methods. Adult male Wistar rats were used to model either (i) facial nerve injury (axotomy) or (ii) reinjury (chronic axotomy followed by a second axotomy within 5 months). The rats were housed in the animal facility of the Eye and ENT Hospital of Shanghai Medical School, Fudan University (Shanghai, China). Expression of Shh (sonic hedgehog) and growth-associated protein 43 (GAP43, a neuronal marker) was detected in bilateral facial nuclei using reverse transcriptase PCR, western blotting analysis, and immunohistochemistry. The number of surviving motoneurons was quantified, and facial nerve regeneration was examined using transmission electron microscopy. Results. Reinjury of the facial nerve 12 weeks after the first axotomy resulted in upregulation of GAP43 mRNA and protein expression in neurons ipsilateral to the axotomy; immunohistochemistry revealed that Shh expression was higher compared with control side facial nuclei at the same time point. GAP43 expression subsequently decreased. Conclusion. The greatest regeneration potential of the facial nerve occurred within 5 months following chronic axotomy in rats, and regeneration may involve the Shh signaling pathway. |
| format | Article |
| id | doaj-art-72c1f5543be04c56830c4e01263db1a1 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-72c1f5543be04c56830c4e01263db1a12025-02-03T05:52:43ZengWileyNeural Plasticity2090-59041687-54432020-01-01202010.1155/2020/88845118884511The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in RatsYusu Ni0Diyan Chen1Yi Jiang2Danhong Qiu3Wen Li4Huawei Li5Otology and Skull Base Surgery Department, Eye and ENT Hospital of Shanghai Medical School, Fudan University, ChinaOtology and Skull Base Surgery Department, Eye and ENT Hospital of Shanghai Medical School, Fudan University, ChinaDepartment of Ophthalmology, Shanghai Xin Shi Jie Eye Hospital, Shanghai, ChinaOtolaryngology Department, Pudong Hospital, Shanghai, ChinaCentral Laboratory, Eye and ENT Hospital of Shanghai Medical School, Fudan University, ChinaOtology and Skull Base Surgery Department, Eye and ENT Hospital of Shanghai Medical School, Fudan University, ChinaBackground. The precise mechanisms of nerve regeneration remain unclear. The potential of facial nerve regeneration and probable mechanisms involved following chronic facial nerve injury should be further studied. Methods. Adult male Wistar rats were used to model either (i) facial nerve injury (axotomy) or (ii) reinjury (chronic axotomy followed by a second axotomy within 5 months). The rats were housed in the animal facility of the Eye and ENT Hospital of Shanghai Medical School, Fudan University (Shanghai, China). Expression of Shh (sonic hedgehog) and growth-associated protein 43 (GAP43, a neuronal marker) was detected in bilateral facial nuclei using reverse transcriptase PCR, western blotting analysis, and immunohistochemistry. The number of surviving motoneurons was quantified, and facial nerve regeneration was examined using transmission electron microscopy. Results. Reinjury of the facial nerve 12 weeks after the first axotomy resulted in upregulation of GAP43 mRNA and protein expression in neurons ipsilateral to the axotomy; immunohistochemistry revealed that Shh expression was higher compared with control side facial nuclei at the same time point. GAP43 expression subsequently decreased. Conclusion. The greatest regeneration potential of the facial nerve occurred within 5 months following chronic axotomy in rats, and regeneration may involve the Shh signaling pathway.http://dx.doi.org/10.1155/2020/8884511 |
| spellingShingle | Yusu Ni Diyan Chen Yi Jiang Danhong Qiu Wen Li Huawei Li The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats Neural Plasticity |
| title | The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats |
| title_full | The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats |
| title_fullStr | The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats |
| title_full_unstemmed | The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats |
| title_short | The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats |
| title_sort | regenerative potential of facial nerve motoneurons following chronic axotomy in rats |
| url | http://dx.doi.org/10.1155/2020/8884511 |
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