Decreases in mRNA by an siRNA Complex Do not Alter Blood Pressure in Mice
Recent genomewide association studies of large samples have identified genes that are associated with blood pressure. The Global Blood Pressure Genetics (Global BPgen) and Cohorts for Heart and Aging Research in Genome Epidemiology (CHARGE) consortiums identified 14 loci that govern blood pressure o...
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| Format: | Article |
| Language: | English |
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BioMed Central
2012-03-01
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| Series: | Genomics & Informatics |
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| Online Access: | http://genominfo.org/upload/pdf/gni-10-40.pdf |
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| author | Su-Min Ji Young-Bin Shin So-Yon Park Hyeon-Ju Lee Bermseok Oh |
| author_facet | Su-Min Ji Young-Bin Shin So-Yon Park Hyeon-Ju Lee Bermseok Oh |
| author_sort | Su-Min Ji |
| collection | DOAJ |
| description | Recent genomewide association studies of large samples have identified genes that are associated with blood pressure. The Global Blood Pressure Genetics (Global BPgen) and Cohorts for Heart and Aging Research in Genome Epidemiology (CHARGE) consortiums identified 14 loci that govern blood pressure on a genomewide significance level, one of which is CASZ1 confirmed in both Europeans and Asians. CASZ1 is a zinc finger transcription factor that controls apoptosis and cell fate and suppresses neuroblastoma tumor growth by reprogramming gene expression, like a tumor suppressor. To validate the function of CASZ1 in blood pressure, we decreased Casz1 mRNA levels in mice by siRNA. Casz1 siRNA reduced mRNA levels by 59% in a mouse cell line. A polyethylenimine-mixed siRNA complex was injected into mouse tail veins, reducing Casz1 mRNA expression to 45% in the kidney. However, blood pressure in the treated mice was unaffected, despite a 55% reduction in Casz1 mRNA levels in the kidney on multiple siRNA injections daily. Even though Casz1 siRNA-treated mice did not experience any significant change in blood pressure, our study demonstrates the value of in vivo siRNA injection in analyzing the function of candidate genes identified by genomewide association studies. |
| format | Article |
| id | doaj-art-72babbec393c4063b89001d02b5fce9f |
| institution | Kabale University |
| issn | 1598-866X 2234-0742 |
| language | English |
| publishDate | 2012-03-01 |
| publisher | BioMed Central |
| record_format | Article |
| series | Genomics & Informatics |
| spelling | doaj-art-72babbec393c4063b89001d02b5fce9f2025-02-02T22:51:45ZengBioMed CentralGenomics & Informatics1598-866X2234-07422012-03-01101404310.5808/GI.2012.10.1.4035Decreases in mRNA by an siRNA Complex Do not Alter Blood Pressure in MiceSu-Min Ji0Young-Bin Shin1So-Yon Park2Hyeon-Ju Lee3Bermseok Oh4Department of Biomedical Engineering, Kyung Hee University School of Medicine, Seoul 130-702, Korea.Department of Biomedical Engineering, Kyung Hee University School of Medicine, Seoul 130-702, Korea.Department of Biomedical Engineering, Kyung Hee University School of Medicine, Seoul 130-702, Korea.Department of Biomedical Engineering, Kyung Hee University School of Medicine, Seoul 130-702, Korea.Department of Biomedical Engineering, Kyung Hee University School of Medicine, Seoul 130-702, Korea.Recent genomewide association studies of large samples have identified genes that are associated with blood pressure. The Global Blood Pressure Genetics (Global BPgen) and Cohorts for Heart and Aging Research in Genome Epidemiology (CHARGE) consortiums identified 14 loci that govern blood pressure on a genomewide significance level, one of which is CASZ1 confirmed in both Europeans and Asians. CASZ1 is a zinc finger transcription factor that controls apoptosis and cell fate and suppresses neuroblastoma tumor growth by reprogramming gene expression, like a tumor suppressor. To validate the function of CASZ1 in blood pressure, we decreased Casz1 mRNA levels in mice by siRNA. Casz1 siRNA reduced mRNA levels by 59% in a mouse cell line. A polyethylenimine-mixed siRNA complex was injected into mouse tail veins, reducing Casz1 mRNA expression to 45% in the kidney. However, blood pressure in the treated mice was unaffected, despite a 55% reduction in Casz1 mRNA levels in the kidney on multiple siRNA injections daily. Even though Casz1 siRNA-treated mice did not experience any significant change in blood pressure, our study demonstrates the value of in vivo siRNA injection in analyzing the function of candidate genes identified by genomewide association studies.http://genominfo.org/upload/pdf/gni-10-40.pdfassociation analysisblood pressureCASZ1expression |
| spellingShingle | Su-Min Ji Young-Bin Shin So-Yon Park Hyeon-Ju Lee Bermseok Oh Decreases in mRNA by an siRNA Complex Do not Alter Blood Pressure in Mice Genomics & Informatics association analysis blood pressure CASZ1 expression |
| title | Decreases in mRNA by an siRNA Complex Do not Alter Blood Pressure in Mice |
| title_full | Decreases in mRNA by an siRNA Complex Do not Alter Blood Pressure in Mice |
| title_fullStr | Decreases in mRNA by an siRNA Complex Do not Alter Blood Pressure in Mice |
| title_full_unstemmed | Decreases in mRNA by an siRNA Complex Do not Alter Blood Pressure in Mice |
| title_short | Decreases in mRNA by an siRNA Complex Do not Alter Blood Pressure in Mice |
| title_sort | decreases in mrna by an sirna complex do not alter blood pressure in mice |
| topic | association analysis blood pressure CASZ1 expression |
| url | http://genominfo.org/upload/pdf/gni-10-40.pdf |
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