Bioactive bacterial nanocellulose membranes for non-surgical debridement and infection prevention in burn wound healing
Novel bioactive bacterial nanocellulose (BnC) membranes were developed for effective, non-surgical debridement and infection-prevention in burn wound healing. Membranes were modified in situ with carboxymethyl cellulose (CMC) and ex situ with the proteolytic enzyme bromelain (Br) and antimicrobial p...
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Elsevier
2025-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266689392500101X |
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| author | Urška Jančič Isabella Nacu Liliana Verestiuc Fiorenza Rancan Selestina Gorgieva |
| author_facet | Urška Jančič Isabella Nacu Liliana Verestiuc Fiorenza Rancan Selestina Gorgieva |
| author_sort | Urška Jančič |
| collection | DOAJ |
| description | Novel bioactive bacterial nanocellulose (BnC) membranes were developed for effective, non-surgical debridement and infection-prevention in burn wound healing. Membranes were modified in situ with carboxymethyl cellulose (CMC) and ex situ with the proteolytic enzyme bromelain (Br) and antimicrobial peptide nisin (N). Post-processing into stable cellulose nanocrystal dispersions (ζ = -26 mV), enables assembly of model films for demonstration of high, irreversible bromelain (95 %) and nisin (99.5 %) adsorption. The BnC-CMC and BnC-CMC-N membranes were in vitro cytocompatible for HaCaT cells and induced faster cell proliferation with cell viability exceeding 100 % after 24 h incubation. The innovative aspect of this study lies in the ex vivo evaluation using an advanced human skin explant model with induced burns, providing a realistic, physiologically relevant assessment of membrane performance. Ex vivo experiments indicated the cytocompatibility of the BnC-CMC membrane with no acute toxicity or skin irritation, while nisin presence resulted in moderate irritating effect. Notably, the BnC-CMC-Br membrane showed digestion of intercellular junctions in the epidermis, while not inducing acute toxicity and skin irritation. By leveraging this innovative ex vivo human skin model in novel BnC-based membranes testing, the study provides a crucial translational step, bridging in vitro assessments and clinical applications for burn wound treatment. |
| format | Article |
| id | doaj-art-72a00b7d3a4c480b8c117efae6b81c20 |
| institution | DOAJ |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-72a00b7d3a4c480b8c117efae6b81c202025-08-20T03:20:57ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-06-011010076210.1016/j.carpta.2025.100762Bioactive bacterial nanocellulose membranes for non-surgical debridement and infection prevention in burn wound healingUrška Jančič0Isabella Nacu1Liliana Verestiuc2Fiorenza Rancan3Selestina Gorgieva4University of Maribor, Faculty of Mechanical Engineering, Smetanova ulica 17, 2000, Maribor, SloveniaGrigore T. Popa University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biomedical Sciences, 9-13 Kogalniceanu Street, 700454 Iasi, RomaniaGrigore T. Popa University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biomedical Sciences, 9-13 Kogalniceanu Street, 700454 Iasi, RomaniaCharité – Universitätsmedizin Berlin, Clinical Research Center for Hair and Skin Science, Department of Dermatology, Venereology und Allergology, Charitéplatz 1, Berlin, GermanyUniversity of Maribor, Faculty of Mechanical Engineering, Smetanova ulica 17, 2000, Maribor, Slovenia; Corresponding author at: University of Maribor, Faculty of Mechanical Engineering, Smetanova 17, 2000 Maribor, Slovenia.Novel bioactive bacterial nanocellulose (BnC) membranes were developed for effective, non-surgical debridement and infection-prevention in burn wound healing. Membranes were modified in situ with carboxymethyl cellulose (CMC) and ex situ with the proteolytic enzyme bromelain (Br) and antimicrobial peptide nisin (N). Post-processing into stable cellulose nanocrystal dispersions (ζ = -26 mV), enables assembly of model films for demonstration of high, irreversible bromelain (95 %) and nisin (99.5 %) adsorption. The BnC-CMC and BnC-CMC-N membranes were in vitro cytocompatible for HaCaT cells and induced faster cell proliferation with cell viability exceeding 100 % after 24 h incubation. The innovative aspect of this study lies in the ex vivo evaluation using an advanced human skin explant model with induced burns, providing a realistic, physiologically relevant assessment of membrane performance. Ex vivo experiments indicated the cytocompatibility of the BnC-CMC membrane with no acute toxicity or skin irritation, while nisin presence resulted in moderate irritating effect. Notably, the BnC-CMC-Br membrane showed digestion of intercellular junctions in the epidermis, while not inducing acute toxicity and skin irritation. By leveraging this innovative ex vivo human skin model in novel BnC-based membranes testing, the study provides a crucial translational step, bridging in vitro assessments and clinical applications for burn wound treatment.http://www.sciencedirect.com/science/article/pii/S266689392500101XBacterial nanocelluloseBromelainNisinCarboxymethyl celluloseBioactiveBurn wound treatment |
| spellingShingle | Urška Jančič Isabella Nacu Liliana Verestiuc Fiorenza Rancan Selestina Gorgieva Bioactive bacterial nanocellulose membranes for non-surgical debridement and infection prevention in burn wound healing Carbohydrate Polymer Technologies and Applications Bacterial nanocellulose Bromelain Nisin Carboxymethyl cellulose Bioactive Burn wound treatment |
| title | Bioactive bacterial nanocellulose membranes for non-surgical debridement and infection prevention in burn wound healing |
| title_full | Bioactive bacterial nanocellulose membranes for non-surgical debridement and infection prevention in burn wound healing |
| title_fullStr | Bioactive bacterial nanocellulose membranes for non-surgical debridement and infection prevention in burn wound healing |
| title_full_unstemmed | Bioactive bacterial nanocellulose membranes for non-surgical debridement and infection prevention in burn wound healing |
| title_short | Bioactive bacterial nanocellulose membranes for non-surgical debridement and infection prevention in burn wound healing |
| title_sort | bioactive bacterial nanocellulose membranes for non surgical debridement and infection prevention in burn wound healing |
| topic | Bacterial nanocellulose Bromelain Nisin Carboxymethyl cellulose Bioactive Burn wound treatment |
| url | http://www.sciencedirect.com/science/article/pii/S266689392500101X |
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