Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells
Age-related macular degeneration (AMD) is a leading cause of legal blindness in individuals over 60 years of age, characterized by the dysfunction of retinal pigmented epithelium cells, specifically in the macular area. Despite several treatment options, AMD therapy remains difficult, especially for...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/946090 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548823344349184 |
|---|---|
| author | Raffaele Nuzzi Monica Gunetti Deborah Rustichelli Barbara Roagna Francesca Fronticelli Bardelli Franca Fagioli Ivana Ferrero |
| author_facet | Raffaele Nuzzi Monica Gunetti Deborah Rustichelli Barbara Roagna Francesca Fronticelli Bardelli Franca Fagioli Ivana Ferrero |
| author_sort | Raffaele Nuzzi |
| collection | DOAJ |
| description | Age-related macular degeneration (AMD) is a leading cause of legal blindness in individuals over 60 years of age, characterized by the dysfunction of retinal pigmented epithelium cells, specifically in the macular area. Despite several treatment options, AMD therapy remains difficult, especially for exudative AMD. Multipotent mesenchymal stem cells (MSCs), with great plasticity and immunomodulant properties, are a promising cell source for cellular therapy and tissue engineering. We evaluated the effects of steroid drugs, often used to treat AMD, in association with MSCs, in view of a possible application together to treat AMD. Morphology, viability, growth kinetics, and immunophenotype were evaluated on healthy donors’ MSCs, treated with triamcinolone acetonide, alcohol-free triamcinolone acetonide, micronized intravitreal triamcinolone and dexamethasone at different concentrations, and in a human retinal pigment epithelial cell line supernatant (ARPE-19). The morphological analysis of MSCs in their standard medium showed a negative correlation with drug concentrations, due to the numerous crystals. Dexamethasone was the least toxic corticosteroid used in this study. ARPE-19 seemed to help cells preserve the typical MSC morphology. In conclusion, this in vitro study demonstrated that high doses of corticosteroid drugs have a negative effect on MSCs, reduced in the presence of a conditioned media. |
| format | Article |
| id | doaj-art-7298bb81a64441c1935013850b8d317f |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-7298bb81a64441c1935013850b8d317f2025-02-03T06:12:56ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/946090946090Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem CellsRaffaele Nuzzi0Monica Gunetti1Deborah Rustichelli2Barbara Roagna3Francesca Fronticelli Bardelli4Franca Fagioli5Ivana Ferrero6Department of Clinical Pathophysiology, Ophthalmology Section, University of Turin, 10126 Turin, ItalyPediatric Onco-Hematology, Stem Cell Transplantation and Cellular Therapy Division, Regina Margherita Children’s Hospital, 10126 Turin, ItalyPediatric Onco-Hematology, Stem Cell Transplantation and Cellular Therapy Division, Regina Margherita Children’s Hospital, 10126 Turin, ItalyDepartment of Clinical Pathophysiology, Ophthalmology Section, University of Turin, 10126 Turin, ItalyDepartment of Clinical Pathophysiology, Ophthalmology Section, University of Turin, 10126 Turin, ItalyPediatric Onco-Hematology, Stem Cell Transplantation and Cellular Therapy Division, Regina Margherita Children’s Hospital, 10126 Turin, ItalyPediatric Onco-Hematology, Stem Cell Transplantation and Cellular Therapy Division, Regina Margherita Children’s Hospital, 10126 Turin, ItalyAge-related macular degeneration (AMD) is a leading cause of legal blindness in individuals over 60 years of age, characterized by the dysfunction of retinal pigmented epithelium cells, specifically in the macular area. Despite several treatment options, AMD therapy remains difficult, especially for exudative AMD. Multipotent mesenchymal stem cells (MSCs), with great plasticity and immunomodulant properties, are a promising cell source for cellular therapy and tissue engineering. We evaluated the effects of steroid drugs, often used to treat AMD, in association with MSCs, in view of a possible application together to treat AMD. Morphology, viability, growth kinetics, and immunophenotype were evaluated on healthy donors’ MSCs, treated with triamcinolone acetonide, alcohol-free triamcinolone acetonide, micronized intravitreal triamcinolone and dexamethasone at different concentrations, and in a human retinal pigment epithelial cell line supernatant (ARPE-19). The morphological analysis of MSCs in their standard medium showed a negative correlation with drug concentrations, due to the numerous crystals. Dexamethasone was the least toxic corticosteroid used in this study. ARPE-19 seemed to help cells preserve the typical MSC morphology. In conclusion, this in vitro study demonstrated that high doses of corticosteroid drugs have a negative effect on MSCs, reduced in the presence of a conditioned media.http://dx.doi.org/10.1155/2012/946090 |
| spellingShingle | Raffaele Nuzzi Monica Gunetti Deborah Rustichelli Barbara Roagna Francesca Fronticelli Bardelli Franca Fagioli Ivana Ferrero Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells Stem Cells International |
| title | Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells |
| title_full | Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells |
| title_fullStr | Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells |
| title_full_unstemmed | Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells |
| title_short | Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells |
| title_sort | effect of in vitro exposure of corticosteroid drugs conventionally used in amd treatment on mesenchymal stem cells |
| url | http://dx.doi.org/10.1155/2012/946090 |
| work_keys_str_mv | AT raffaelenuzzi effectofinvitroexposureofcorticosteroiddrugsconventionallyusedinamdtreatmentonmesenchymalstemcells AT monicagunetti effectofinvitroexposureofcorticosteroiddrugsconventionallyusedinamdtreatmentonmesenchymalstemcells AT deborahrustichelli effectofinvitroexposureofcorticosteroiddrugsconventionallyusedinamdtreatmentonmesenchymalstemcells AT barbararoagna effectofinvitroexposureofcorticosteroiddrugsconventionallyusedinamdtreatmentonmesenchymalstemcells AT francescafronticellibardelli effectofinvitroexposureofcorticosteroiddrugsconventionallyusedinamdtreatmentonmesenchymalstemcells AT francafagioli effectofinvitroexposureofcorticosteroiddrugsconventionallyusedinamdtreatmentonmesenchymalstemcells AT ivanaferrero effectofinvitroexposureofcorticosteroiddrugsconventionallyusedinamdtreatmentonmesenchymalstemcells |