Polyhydroxyalkanoates Biopolymer Production by Moderately Halophilic Paracoccus onubensis Strain E3: Extraction, Characterization and Synergistic Activity with Sorafenib Drug against Hepatocellular Carcinoma through Molecular Docking Approach
Polyhydroxyalkanoates (PHAs) are biodegradable, low-cost, and ecofriendly polymers produced by various bacteria in the environment. The aim of this study was to investigate the use of moderately halophilic Paracoccus onubensis strain E3 as a promising PHA-producing bacteria isolated from a hypersa...
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The National Information and Documentation Centre (NIDOC) , Egypt
2025-01-01
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| Series: | Novel Research in Microbiology Journal |
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| author | Hend A. Hamedo Ahmed E.M. Shokr Omnia T. Abd-Elsalam Naglaa Elshafey |
| author_facet | Hend A. Hamedo Ahmed E.M. Shokr Omnia T. Abd-Elsalam Naglaa Elshafey |
| author_sort | Hend A. Hamedo |
| collection | DOAJ |
| description | Polyhydroxyalkanoates (PHAs) are biodegradable, low-cost, and ecofriendly polymers produced
by various bacteria in the environment. The aim of this study was to investigate the use of moderately
halophilic Paracoccus onubensis strain E3 as a promising PHA-producing bacteria isolated from a hypersaline environment in Egypt. The optimum conditions for PHAs production were explored and the recorded
maximum yield of PHAs was 54.77 mg/ l after 72 h of incubation at 37 o
C, pH 7, and 4 % NaCl (w/v), using
constant carbon and nitrogen sources. PHAs were extracted and then subjected to Fourier transform infrared
spectroscopy (FT-IR), where the spectrum showed a strong band at 1649.72 cm−1 for the carbon (C=O)
stretching of the ester group, which is a common feature in PHAs structures, and Gas chromatography–mass
spectrometry (GC-MS) analyses. The GC-MS identified twelve different biodegradable PHA compounds,
including hexadecenoic acid and octadecanoic acid. The molecular docking study displayed high potential of
the extracted PHA ingredients as drugs used against liver hepatocellular carcinoma (HCC); with binding
affinity ranging between -4.0 to -6.5 kcal/ mol. This is in addition to the prediction of a synergistic effect
of PHAs ingredients and sorafenib drug against HCC, showing a promising binding affinity ranging from
20.2 to 23.2 kcal/ mol. Our findings indicate that Paracoccus onubensis strain E3 is a promising candidate for
producing PHAs, which could be used as a drug delivery system for HCC treatment with sorafenib drug. |
| format | Article |
| id | doaj-art-728b1ba96d86480dbea33889eaa8c452 |
| institution | Kabale University |
| issn | 2537-0286 2537-0294 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj-art-728b1ba96d86480dbea33889eaa8c4522025-02-04T11:53:56ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942025-01-01911327https://dx.doi.org/10.17582/journal.NRMJ/2025/9.1.13.27Polyhydroxyalkanoates Biopolymer Production by Moderately Halophilic Paracoccus onubensis Strain E3: Extraction, Characterization and Synergistic Activity with Sorafenib Drug against Hepatocellular Carcinoma through Molecular Docking ApproachHend A. Hamedo0Ahmed E.M. Shokr1Omnia T. Abd-Elsalam2Naglaa Elshafey3Botany and Microbiology Department, Faculty of Science, Arish University, Al-Arish 45511, EgyptBotany and Microbiology Department, Faculty of Science, Arish University, Al-Arish 45511, EgyptBotany and Microbiology Department, Faculty of Science, Arish University, Al-Arish 45511, EgyptBotany and Microbiology Department, Faculty of Science, Arish University, Al-Arish 45511, EgyptPolyhydroxyalkanoates (PHAs) are biodegradable, low-cost, and ecofriendly polymers produced by various bacteria in the environment. The aim of this study was to investigate the use of moderately halophilic Paracoccus onubensis strain E3 as a promising PHA-producing bacteria isolated from a hypersaline environment in Egypt. The optimum conditions for PHAs production were explored and the recorded maximum yield of PHAs was 54.77 mg/ l after 72 h of incubation at 37 o C, pH 7, and 4 % NaCl (w/v), using constant carbon and nitrogen sources. PHAs were extracted and then subjected to Fourier transform infrared spectroscopy (FT-IR), where the spectrum showed a strong band at 1649.72 cm−1 for the carbon (C=O) stretching of the ester group, which is a common feature in PHAs structures, and Gas chromatography–mass spectrometry (GC-MS) analyses. The GC-MS identified twelve different biodegradable PHA compounds, including hexadecenoic acid and octadecanoic acid. The molecular docking study displayed high potential of the extracted PHA ingredients as drugs used against liver hepatocellular carcinoma (HCC); with binding affinity ranging between -4.0 to -6.5 kcal/ mol. This is in addition to the prediction of a synergistic effect of PHAs ingredients and sorafenib drug against HCC, showing a promising binding affinity ranging from 20.2 to 23.2 kcal/ mol. Our findings indicate that Paracoccus onubensis strain E3 is a promising candidate for producing PHAs, which could be used as a drug delivery system for HCC treatment with sorafenib drug. https://researcherslinks.com/current-issues/Polyhydroxyalkanoates-Biopolymer-Production/44/1/10510/htmlpolyhydroxyalkanoatesparacoccus onubensissolar salternmolecular dockinghepatocellular carcinoma |
| spellingShingle | Hend A. Hamedo Ahmed E.M. Shokr Omnia T. Abd-Elsalam Naglaa Elshafey Polyhydroxyalkanoates Biopolymer Production by Moderately Halophilic Paracoccus onubensis Strain E3: Extraction, Characterization and Synergistic Activity with Sorafenib Drug against Hepatocellular Carcinoma through Molecular Docking Approach Novel Research in Microbiology Journal polyhydroxyalkanoates paracoccus onubensis solar saltern molecular docking hepatocellular carcinoma |
| title | Polyhydroxyalkanoates Biopolymer Production by Moderately Halophilic Paracoccus onubensis Strain E3: Extraction, Characterization and Synergistic Activity with Sorafenib Drug against Hepatocellular Carcinoma through Molecular Docking Approach |
| title_full | Polyhydroxyalkanoates Biopolymer Production by Moderately Halophilic Paracoccus onubensis Strain E3: Extraction, Characterization and Synergistic Activity with Sorafenib Drug against Hepatocellular Carcinoma through Molecular Docking Approach |
| title_fullStr | Polyhydroxyalkanoates Biopolymer Production by Moderately Halophilic Paracoccus onubensis Strain E3: Extraction, Characterization and Synergistic Activity with Sorafenib Drug against Hepatocellular Carcinoma through Molecular Docking Approach |
| title_full_unstemmed | Polyhydroxyalkanoates Biopolymer Production by Moderately Halophilic Paracoccus onubensis Strain E3: Extraction, Characterization and Synergistic Activity with Sorafenib Drug against Hepatocellular Carcinoma through Molecular Docking Approach |
| title_short | Polyhydroxyalkanoates Biopolymer Production by Moderately Halophilic Paracoccus onubensis Strain E3: Extraction, Characterization and Synergistic Activity with Sorafenib Drug against Hepatocellular Carcinoma through Molecular Docking Approach |
| title_sort | polyhydroxyalkanoates biopolymer production by moderately halophilic paracoccus onubensis strain e3 extraction characterization and synergistic activity with sorafenib drug against hepatocellular carcinoma through molecular docking approach |
| topic | polyhydroxyalkanoates paracoccus onubensis solar saltern molecular docking hepatocellular carcinoma |
| url | https://researcherslinks.com/current-issues/Polyhydroxyalkanoates-Biopolymer-Production/44/1/10510/html |
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