Rapid Acquisition of High-Pixel Fluorescence Lifetime Images of Living Cells via Image Reconstruction Based on Edge-Preserving Interpolation

Rapid Acquisition of High-Pixel Fluorescence Lifetime Images of Living Cells via Image Reconstruction Based on Edge-Preserving Interpolation

Fluorescence lifetime imaging (FLIM) has established itself as a pivotal tool for investigating biological processes within living cells. However, the extensive imaging duration necessary to accumulate sufficient photons for accurate fluorescence lifetime calculations poses a significant obstacle to...

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Bibliographic Details
Main Authors: Yinru Zhu, Yong Guo, Xinwei Gao, Qinglin Chen, Yingying Chen, Ruijie Xiang, Baichang Lin, Luwei Wang, Yuan Lu, Wei Yan
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Biosensors
Subjects:
fluorescence lifetime imaging
image reconstruction
live-cell imaging
high-pixel image
edge-preserving interpolation method
Online Access:https://www.mdpi.com/2079-6374/15/1/43
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