A Novel Approach for the Enumeration of Peripheral Blood Stem Cells Suitable for Transplantation
Stem cells have the capability to proliferate and differentiate into various cells of the body. Few stem cell sources have been approved for transplantation, among them are the hematopoietic progenitor cells which are progenitors of the myeloid and erythroid lineage in the hematopoietic system, that...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Transplantation |
| Online Access: | http://dx.doi.org/10.1155/2014/473503 |
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| _version_ | 1832551678775132160 |
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| author | Winston Costa Pereira Omar Alsuhaibani Ghaleb Elyamany Abdulaziz Al Abdulaaly |
| author_facet | Winston Costa Pereira Omar Alsuhaibani Ghaleb Elyamany Abdulaziz Al Abdulaaly |
| author_sort | Winston Costa Pereira |
| collection | DOAJ |
| description | Stem cells have the capability to proliferate and differentiate into various cells of the body. Few stem cell sources have been approved for transplantation, among them are the hematopoietic progenitor cells which are progenitors of the myeloid and erythroid lineage in the hematopoietic system, that continually provides mature blood cells during the lifespan of the individual. These well-characterized stem cells are clinically relevant in the treatment of diseases such as breast cancer, leukemias, and congenital immunodeficiencies. Peripheral blood stem transplantation is a standard procedure after its first successful transplantation more than 35 years ago. The minimum intended dose of stem cells given to the patient is 2.5×106 –5×106 cells. In this study, we are establishing a correlation between the number of stem cells enumerated and the weight of the patient as a determinant for suitable transplantation. We have established a conversion factor to deliver the required dose of approximately 3×106 stem cells/kg body weight. This will ensure a uniform collection strategy that is sufficient for transplantation irrespective of the weight of the patient. This approach, if incorporated, will lead to a significantly lesser rate of bone marrow transplantation failures as sufficient number of stem cells will ensure engraftment of stem cells. |
| format | Article |
| id | doaj-art-725468eba69b49f8b68d15d54d9d50d6 |
| institution | Kabale University |
| issn | 2090-0007 2090-0015 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Transplantation |
| spelling | doaj-art-725468eba69b49f8b68d15d54d9d50d62025-02-03T06:00:51ZengWileyJournal of Transplantation2090-00072090-00152014-01-01201410.1155/2014/473503473503A Novel Approach for the Enumeration of Peripheral Blood Stem Cells Suitable for TransplantationWinston Costa Pereira0Omar Alsuhaibani1Ghaleb Elyamany2Abdulaziz Al Abdulaaly3Stem Cell Section, Department of CML and Blood Bank, Prince Sultan Military Medical City, P.O. Box 7897, Riyadh 11159, Saudi ArabiaStem Cell Section, Department of CML and Blood Bank, Prince Sultan Military Medical City, P.O. Box 7897, Riyadh 11159, Saudi ArabiaStem Cell Section, Department of CML and Blood Bank, Prince Sultan Military Medical City, P.O. Box 7897, Riyadh 11159, Saudi ArabiaStem Cell Section, Department of CML and Blood Bank, Prince Sultan Military Medical City, P.O. Box 7897, Riyadh 11159, Saudi ArabiaStem cells have the capability to proliferate and differentiate into various cells of the body. Few stem cell sources have been approved for transplantation, among them are the hematopoietic progenitor cells which are progenitors of the myeloid and erythroid lineage in the hematopoietic system, that continually provides mature blood cells during the lifespan of the individual. These well-characterized stem cells are clinically relevant in the treatment of diseases such as breast cancer, leukemias, and congenital immunodeficiencies. Peripheral blood stem transplantation is a standard procedure after its first successful transplantation more than 35 years ago. The minimum intended dose of stem cells given to the patient is 2.5×106 –5×106 cells. In this study, we are establishing a correlation between the number of stem cells enumerated and the weight of the patient as a determinant for suitable transplantation. We have established a conversion factor to deliver the required dose of approximately 3×106 stem cells/kg body weight. This will ensure a uniform collection strategy that is sufficient for transplantation irrespective of the weight of the patient. This approach, if incorporated, will lead to a significantly lesser rate of bone marrow transplantation failures as sufficient number of stem cells will ensure engraftment of stem cells.http://dx.doi.org/10.1155/2014/473503 |
| spellingShingle | Winston Costa Pereira Omar Alsuhaibani Ghaleb Elyamany Abdulaziz Al Abdulaaly A Novel Approach for the Enumeration of Peripheral Blood Stem Cells Suitable for Transplantation Journal of Transplantation |
| title | A Novel Approach for the Enumeration of Peripheral Blood Stem Cells Suitable for Transplantation |
| title_full | A Novel Approach for the Enumeration of Peripheral Blood Stem Cells Suitable for Transplantation |
| title_fullStr | A Novel Approach for the Enumeration of Peripheral Blood Stem Cells Suitable for Transplantation |
| title_full_unstemmed | A Novel Approach for the Enumeration of Peripheral Blood Stem Cells Suitable for Transplantation |
| title_short | A Novel Approach for the Enumeration of Peripheral Blood Stem Cells Suitable for Transplantation |
| title_sort | novel approach for the enumeration of peripheral blood stem cells suitable for transplantation |
| url | http://dx.doi.org/10.1155/2014/473503 |
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