Fatty acids profile in Awassi sheep milk affected by some genes' single and combined effects.
Objective: This study intended to figure out the effects of fixed effects and genes such as beta-lactoglobulin (β-LG), prolactin (PRL), annexin A9 (ANXA9), and acetyl-CoA acyltransferase 2 (ACAA2) on the structure of fatty milk acids in Awassi ewes, as well as any potential genotype-genotype interac...
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| Language: | English |
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Network for the Veterinarians of Bangladesh
2025-03-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=182698 |
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| author | Khaleel I. Jawasreh Dana Khrais Muhammad Alu'datt Nizar J. Haddad Sami Awabdeh Mohammad Isam AlMadani Mohamad Brake Mohamad Ahmad Al-Araishi Monther Sadder Ahmad Al-Amareen. |
| author_facet | Khaleel I. Jawasreh Dana Khrais Muhammad Alu'datt Nizar J. Haddad Sami Awabdeh Mohammad Isam AlMadani Mohamad Brake Mohamad Ahmad Al-Araishi Monther Sadder Ahmad Al-Amareen. |
| author_sort | Khaleel I. Jawasreh |
| collection | DOAJ |
| description | Objective: This study intended to figure out the effects of fixed effects and genes such as beta-lactoglobulin (β-LG), prolactin (PRL), annexin A9 (ANXA9), and acetyl-CoA acyltransferase 2 (ACAA2) on the structure of fatty milk acids in Awassi ewes, as well as any potential genotype-genotype interactions.
Materials and Methods: Fatty acid (FA) profile and other milk components were examined from 116 Awassi ewes in total. Polymerase chain reaction (PCR) was used to extract and genotype their DNA, and either sequencing or restriction fragment length polymorphism (RFLP) analysis came next.
Results: Saturated, medium-chain, and long-chain FA proportions were not significantly impacted by the genotypes of β-LG, PRL, ANXA9, and ACAA2. Conversely, ewes with the β-LG AA genotype displayed higher levels of monounsaturated fatty acids (MUFAs), while the β-LG AB genotype was linked to the highest levels of polyunsaturated fatty acids (PUFAs). It has been demonstrated that PUFA levels are impacted by single nucleotide polymorphisms (SNPs) of ANXA9, while VLCFA and PUFA levels are changed by SNPs of ACAA2. The PRL AA genotype showed the greatest influence on VLCFA. Furthermore, milk exhibited the highest levels of MUFA and PUFA due to the interac¬tion between β-LG and PRL. The interaction PRL-ANXA9 exhibited the greatest levels of VLCFA. Additionally, Dam lambing weight affected the levels of MUFA, MCFA, and long-chain fatty acids.
Conclusion: To increase the levels of MUFA and PUFA constituents, the selection program should effectively harness and integrate the synergistic benefits of β-LG, PRL, ANXA9, and ACAA2 as well as their interaction. [J Adv Vet Anim Res 2025; 12(1.000): 238-251] |
| format | Article |
| id | doaj-art-722c8bc6dfd04cedbe6f1e5377a22db2 |
| institution | DOAJ |
| issn | 2311-7710 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj-art-722c8bc6dfd04cedbe6f1e5377a22db22025-08-20T03:14:41ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102025-03-0112123825110.5455/javar.2025.l891182698Fatty acids profile in Awassi sheep milk affected by some genes' single and combined effects.Khaleel I. Jawasreh0Dana Khrais1Muhammad Alu'datt2Nizar J. Haddad3Sami Awabdeh4Mohammad Isam AlMadani5Mohamad Brake6Mohamad Ahmad Al-Araishi7Monther Sadder8Ahmad Al-Amareen.9Faculty of Agriculture, Jordan University of Science and Technology (JUST), Irbid, Jordan Faculty of Agriculture, Jordan University of Science and Technology (JUST), Irbid, Jordan Faculty of Agriculture, Jordan University of Science and Technology (JUST), Irbid, Jordan National Agriculture Research Center (NARC), Baqa'a, Jordan. & Innovation and Business Development, Fresh Del Monte, Amman, Jordan National Agriculture Research Center (NARC), Baqa'a, Jordan Qatar University, Qatar University Press, Qatar University, Doha, Qatar Science Faculty, Jerash University, Jerash, Jordan National Agriculture Research Center (NARC), Baqa'a, Jordan Faculty of Agriculture, The University of Jordan, Amman, Jordan National Agriculture Research Center (NARC), Baqa'a, Jordan.Objective: This study intended to figure out the effects of fixed effects and genes such as beta-lactoglobulin (β-LG), prolactin (PRL), annexin A9 (ANXA9), and acetyl-CoA acyltransferase 2 (ACAA2) on the structure of fatty milk acids in Awassi ewes, as well as any potential genotype-genotype interactions. Materials and Methods: Fatty acid (FA) profile and other milk components were examined from 116 Awassi ewes in total. Polymerase chain reaction (PCR) was used to extract and genotype their DNA, and either sequencing or restriction fragment length polymorphism (RFLP) analysis came next. Results: Saturated, medium-chain, and long-chain FA proportions were not significantly impacted by the genotypes of β-LG, PRL, ANXA9, and ACAA2. Conversely, ewes with the β-LG AA genotype displayed higher levels of monounsaturated fatty acids (MUFAs), while the β-LG AB genotype was linked to the highest levels of polyunsaturated fatty acids (PUFAs). It has been demonstrated that PUFA levels are impacted by single nucleotide polymorphisms (SNPs) of ANXA9, while VLCFA and PUFA levels are changed by SNPs of ACAA2. The PRL AA genotype showed the greatest influence on VLCFA. Furthermore, milk exhibited the highest levels of MUFA and PUFA due to the interac¬tion between β-LG and PRL. The interaction PRL-ANXA9 exhibited the greatest levels of VLCFA. Additionally, Dam lambing weight affected the levels of MUFA, MCFA, and long-chain fatty acids. Conclusion: To increase the levels of MUFA and PUFA constituents, the selection program should effectively harness and integrate the synergistic benefits of β-LG, PRL, ANXA9, and ACAA2 as well as their interaction. [J Adv Vet Anim Res 2025; 12(1.000): 238-251]http://www.ejmanager.com/fulltextpdf.php?mno=182698awassi ewes; fatty acids; gene interaction |
| spellingShingle | Khaleel I. Jawasreh Dana Khrais Muhammad Alu'datt Nizar J. Haddad Sami Awabdeh Mohammad Isam AlMadani Mohamad Brake Mohamad Ahmad Al-Araishi Monther Sadder Ahmad Al-Amareen. Fatty acids profile in Awassi sheep milk affected by some genes' single and combined effects. Journal of Advanced Veterinary and Animal Research awassi ewes; fatty acids; gene interaction |
| title | Fatty acids profile in Awassi sheep milk affected by some genes' single and combined effects. |
| title_full | Fatty acids profile in Awassi sheep milk affected by some genes' single and combined effects. |
| title_fullStr | Fatty acids profile in Awassi sheep milk affected by some genes' single and combined effects. |
| title_full_unstemmed | Fatty acids profile in Awassi sheep milk affected by some genes' single and combined effects. |
| title_short | Fatty acids profile in Awassi sheep milk affected by some genes' single and combined effects. |
| title_sort | fatty acids profile in awassi sheep milk affected by some genes apos single and combined effects |
| topic | awassi ewes; fatty acids; gene interaction |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=182698 |
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