Genotyping of Cryptosporidium species in children suffering from diarrhea in Sharkyia Governorate, Egypt
Introduction: The protozoan parasite Cryptosporidium is one of the principal reasons for childhood diarrhea around the world. This work aimed to differentiate Cryptosporidium species among children suffering from diarrhea in Sharkyia Governorate, Egypt. Methodology: A total of 97 fecal specimens...
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The Journal of Infection in Developing Countries
2021-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/14367 |
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| author | Samira Metwally Mohammad Magda Saad Ali Sara Ahmed Abdel-Rahman Raghda Abdelrahman Moustafa Mohamed Hassan Sarhan |
| author_facet | Samira Metwally Mohammad Magda Saad Ali Sara Ahmed Abdel-Rahman Raghda Abdelrahman Moustafa Mohamed Hassan Sarhan |
| author_sort | Samira Metwally Mohammad |
| collection | DOAJ |
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Introduction: The protozoan parasite Cryptosporidium is one of the principal reasons for childhood diarrhea around the world. This work aimed to differentiate Cryptosporidium species among children suffering from diarrhea in Sharkyia Governorate, Egypt.
Methodology: A total of 97 fecal specimens were taken from children suffering from diarrhea, attending Pediatric Clinics of Zagazig University and Al-Ahrar Hospitals. Full history was taken. Stool samples were examined microscopically using modified Ziehl–Neelsen stain for detection of Cryptosporidium oocysts. To identify Cryptosporidium genotypes, positive samples were then subjected to nested Polymerase chain reaction-restriction fragment length polymorphism targeting Cryptosporidium oocyst wall protein gene.
Results: The overall detection rate was 27.8% (27/97) using modified Ziehl–Neelsen stain staining method. Using nested polymerase chain reaction, the gene was amplified in 85.2% (23/27). Restriction fragment length polymorphism analysis revealed that 65.2% (15/23) were Cryptosporidium hominis, 30.4% (7/23) were Cryptosporidium parvum, and one sample was not typed (4.4%). The significant risk factors associated with Cryptosporidium infection in children were animal contact and residence in rural areas.
Conclusions: Cryptosporidium is a common enteric parasite affecting children in Sharkyia Governorate, Egypt, with the predominance of C. hominis genotype in children.
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| format | Article |
| id | doaj-art-71fa3662fa4e441d9c2fbd36b17ab4ce |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2021-10-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-71fa3662fa4e441d9c2fbd36b17ab4ce2025-08-20T02:14:16ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802021-10-01151010.3855/jidc.14367Genotyping of Cryptosporidium species in children suffering from diarrhea in Sharkyia Governorate, EgyptSamira Metwally Mohammad0Magda Saad Ali1Sara Ahmed Abdel-Rahman2Raghda Abdelrahman Moustafa3Mohamed Hassan Sarhan4Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, EgyptMedical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, EgyptMedical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, EgyptMedical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, EgyptMedical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt Introduction: The protozoan parasite Cryptosporidium is one of the principal reasons for childhood diarrhea around the world. This work aimed to differentiate Cryptosporidium species among children suffering from diarrhea in Sharkyia Governorate, Egypt. Methodology: A total of 97 fecal specimens were taken from children suffering from diarrhea, attending Pediatric Clinics of Zagazig University and Al-Ahrar Hospitals. Full history was taken. Stool samples were examined microscopically using modified Ziehl–Neelsen stain for detection of Cryptosporidium oocysts. To identify Cryptosporidium genotypes, positive samples were then subjected to nested Polymerase chain reaction-restriction fragment length polymorphism targeting Cryptosporidium oocyst wall protein gene. Results: The overall detection rate was 27.8% (27/97) using modified Ziehl–Neelsen stain staining method. Using nested polymerase chain reaction, the gene was amplified in 85.2% (23/27). Restriction fragment length polymorphism analysis revealed that 65.2% (15/23) were Cryptosporidium hominis, 30.4% (7/23) were Cryptosporidium parvum, and one sample was not typed (4.4%). The significant risk factors associated with Cryptosporidium infection in children were animal contact and residence in rural areas. Conclusions: Cryptosporidium is a common enteric parasite affecting children in Sharkyia Governorate, Egypt, with the predominance of C. hominis genotype in children. https://jidc.org/index.php/journal/article/view/14367ChildrenCOWP geneCryptosporidiumGenotypingModified Ziehl–Neelsen stainPCR-RFLP |
| spellingShingle | Samira Metwally Mohammad Magda Saad Ali Sara Ahmed Abdel-Rahman Raghda Abdelrahman Moustafa Mohamed Hassan Sarhan Genotyping of Cryptosporidium species in children suffering from diarrhea in Sharkyia Governorate, Egypt Journal of Infection in Developing Countries Children COWP gene Cryptosporidium Genotyping Modified Ziehl–Neelsen stain PCR-RFLP |
| title | Genotyping of Cryptosporidium species in children suffering from diarrhea in Sharkyia Governorate, Egypt |
| title_full | Genotyping of Cryptosporidium species in children suffering from diarrhea in Sharkyia Governorate, Egypt |
| title_fullStr | Genotyping of Cryptosporidium species in children suffering from diarrhea in Sharkyia Governorate, Egypt |
| title_full_unstemmed | Genotyping of Cryptosporidium species in children suffering from diarrhea in Sharkyia Governorate, Egypt |
| title_short | Genotyping of Cryptosporidium species in children suffering from diarrhea in Sharkyia Governorate, Egypt |
| title_sort | genotyping of cryptosporidium species in children suffering from diarrhea in sharkyia governorate egypt |
| topic | Children COWP gene Cryptosporidium Genotyping Modified Ziehl–Neelsen stain PCR-RFLP |
| url | https://jidc.org/index.php/journal/article/view/14367 |
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