Production of an Extract with β-1,4-Xylanase Activity by <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> on a Sonicated Brewer’s Spent Grain Substrate
The <i>Fusarium oxysporum</i> species commonly found in soil include plant and human pathogens, and nonpathogenic species. <i>F. oxysporum</i> grown on lignocellulosic substrates under submerged conditions produces an extracellular enzyme profile with hemicellulolytic and cel...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/1/42 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588485728403456 |
|---|---|
| author | Irma A. Arreola-Cruz Rosalba Troncoso-Rojas Francisco Vásquez-Lara Nina G. Heredia-Sandoval Alma R. Islas-Rubio |
| author_facet | Irma A. Arreola-Cruz Rosalba Troncoso-Rojas Francisco Vásquez-Lara Nina G. Heredia-Sandoval Alma R. Islas-Rubio |
| author_sort | Irma A. Arreola-Cruz |
| collection | DOAJ |
| description | The <i>Fusarium oxysporum</i> species commonly found in soil include plant and human pathogens, and nonpathogenic species. <i>F. oxysporum</i> grown on lignocellulosic substrates under submerged conditions produces an extracellular enzyme profile with hemicellulolytic and cellulolytic activities. Our aim was to produce an extract of <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> with β-1,4-xylanase activity after fermentation on a Brewers’ spent grain (BSG)-containing substrate. We prepared the BSG substrate, with or without sonication, for the submerged fermentation of <i>Fusarium oxysporum</i> previously isolated from local soil and preserved at 4 °C. First, an enriched inoculum was prepared, and later, the production of β-1,4-xylanase using the BSG substrates was monitored for up to 6 or 10 days in the enriched inoculum or in the enzyme extract, respectively. An activity of β-1,4-xylanase 12.0 U/mL (day 3) was obtained in the enriched inoculum with the untreated BSG, remaining constant for 3 days. A significant increase in the activity of this enzyme was observed (day 6), especially in the extract obtained using the sonicated BSG substrate (39 U/mL). Applying ultrasound to the BSG before its use in a submerged fermentation with <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> could be an alternative for producing β-1,4-xylanase. |
| format | Article |
| id | doaj-art-71f87f288c334c6e81815a74ac84ff23 |
| institution | Kabale University |
| issn | 2311-5637 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-71f87f288c334c6e81815a74ac84ff232025-01-24T13:32:10ZengMDPI AGFermentation2311-56372025-01-011114210.3390/fermentation11010042Production of an Extract with β-1,4-Xylanase Activity by <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> on a Sonicated Brewer’s Spent Grain SubstrateIrma A. Arreola-Cruz0Rosalba Troncoso-Rojas1Francisco Vásquez-Lara2Nina G. Heredia-Sandoval3Alma R. Islas-Rubio4Coordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera Gustavo E. Astiazarán Rosas # 46, Col. La Victoria, Hermosillo 83304, MexicoCoordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera Gustavo E. Astiazarán Rosas # 46, Col. La Victoria, Hermosillo 83304, MexicoCoordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera Gustavo E. Astiazarán Rosas # 46, Col. La Victoria, Hermosillo 83304, MexicoCoordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera Gustavo E. Astiazarán Rosas # 46, Col. La Victoria, Hermosillo 83304, MexicoCoordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera Gustavo E. Astiazarán Rosas # 46, Col. La Victoria, Hermosillo 83304, MexicoThe <i>Fusarium oxysporum</i> species commonly found in soil include plant and human pathogens, and nonpathogenic species. <i>F. oxysporum</i> grown on lignocellulosic substrates under submerged conditions produces an extracellular enzyme profile with hemicellulolytic and cellulolytic activities. Our aim was to produce an extract of <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> with β-1,4-xylanase activity after fermentation on a Brewers’ spent grain (BSG)-containing substrate. We prepared the BSG substrate, with or without sonication, for the submerged fermentation of <i>Fusarium oxysporum</i> previously isolated from local soil and preserved at 4 °C. First, an enriched inoculum was prepared, and later, the production of β-1,4-xylanase using the BSG substrates was monitored for up to 6 or 10 days in the enriched inoculum or in the enzyme extract, respectively. An activity of β-1,4-xylanase 12.0 U/mL (day 3) was obtained in the enriched inoculum with the untreated BSG, remaining constant for 3 days. A significant increase in the activity of this enzyme was observed (day 6), especially in the extract obtained using the sonicated BSG substrate (39 U/mL). Applying ultrasound to the BSG before its use in a submerged fermentation with <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> could be an alternative for producing β-1,4-xylanase.https://www.mdpi.com/2311-5637/11/1/42<i>Fusarium oxysporum</i>xylanasebrewer’s spent grainultrasound |
| spellingShingle | Irma A. Arreola-Cruz Rosalba Troncoso-Rojas Francisco Vásquez-Lara Nina G. Heredia-Sandoval Alma R. Islas-Rubio Production of an Extract with β-1,4-Xylanase Activity by <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> on a Sonicated Brewer’s Spent Grain Substrate Fermentation <i>Fusarium oxysporum</i> xylanase brewer’s spent grain ultrasound |
| title | Production of an Extract with β-1,4-Xylanase Activity by <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> on a Sonicated Brewer’s Spent Grain Substrate |
| title_full | Production of an Extract with β-1,4-Xylanase Activity by <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> on a Sonicated Brewer’s Spent Grain Substrate |
| title_fullStr | Production of an Extract with β-1,4-Xylanase Activity by <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> on a Sonicated Brewer’s Spent Grain Substrate |
| title_full_unstemmed | Production of an Extract with β-1,4-Xylanase Activity by <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> on a Sonicated Brewer’s Spent Grain Substrate |
| title_short | Production of an Extract with β-1,4-Xylanase Activity by <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> on a Sonicated Brewer’s Spent Grain Substrate |
| title_sort | production of an extract with β 1 4 xylanase activity by i fusarium oxysporum i f sp i melonis i on a sonicated brewer s spent grain substrate |
| topic | <i>Fusarium oxysporum</i> xylanase brewer’s spent grain ultrasound |
| url | https://www.mdpi.com/2311-5637/11/1/42 |
| work_keys_str_mv | AT irmaaarreolacruz productionofanextractwithb14xylanaseactivitybyifusariumoxysporumifspimelonisionasonicatedbrewersspentgrainsubstrate AT rosalbatroncosorojas productionofanextractwithb14xylanaseactivitybyifusariumoxysporumifspimelonisionasonicatedbrewersspentgrainsubstrate AT franciscovasquezlara productionofanextractwithb14xylanaseactivitybyifusariumoxysporumifspimelonisionasonicatedbrewersspentgrainsubstrate AT ninagherediasandoval productionofanextractwithb14xylanaseactivitybyifusariumoxysporumifspimelonisionasonicatedbrewersspentgrainsubstrate AT almarislasrubio productionofanextractwithb14xylanaseactivitybyifusariumoxysporumifspimelonisionasonicatedbrewersspentgrainsubstrate |