Determination of Mycotoxin Contamination Levels in Rice and Dietary Exposure Assessment
The contamination by aflatoxins, ochratoxin A, and zearalenone of samples of paddy and polished rice stored in silos located in Chiriquí, Panama, was evaluated. A total of 23 samples were extracted using immunoaffinity columns and analyzed by high-performance liquid chromatography (HPLC) with a fluo...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2022/3596768 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554758752174080 |
|---|---|
| author | Jose Troestch Stephany Reyes Aracelly Vega |
| author_facet | Jose Troestch Stephany Reyes Aracelly Vega |
| author_sort | Jose Troestch |
| collection | DOAJ |
| description | The contamination by aflatoxins, ochratoxin A, and zearalenone of samples of paddy and polished rice stored in silos located in Chiriquí, Panama, was evaluated. A total of 23 samples were extracted using immunoaffinity columns and analyzed by high-performance liquid chromatography (HPLC) with a fluorescence detector (FLD) and post-column photochemical derivatization. For the method used, the detection limits were lower than 0.25 μg/Kg for aflatoxins (AFB1, AFB2, AFG1, AFG2) and ochratoxin A and 9.35 μg/Kg for zearalenone; the limits of quantification were between 0.25 and 18.75 μg/Kg, respectively. Of the samples analyzed, all of the paddy rice samples were positive for at least one of the mycotoxins studied, zearalenone being the one found with the highest incidence (90.91%); for the polished rice samples, the mycotoxin with the highest incidence was zearalenone (50%), although in concentrations lower than those established in European legislation (100 μg/Kg). The estimate of the daily zearalenone intake according to the concentrations found was always less than 0.07 μg/Kg/bw. This is the first report on the determination of 6 mycotoxins in rice grains from Panama by the HPLC-FLD methodology. Considering the high incidence of mycotoxins in the analyzed rice samples, regular control in the production process is recommended to improve quality and ascertain its safety. |
| format | Article |
| id | doaj-art-71f847c758a64d9c986d9ae2b377db33 |
| institution | Kabale University |
| issn | 1687-8205 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-71f847c758a64d9c986d9ae2b377db332025-02-03T05:50:38ZengWileyJournal of Toxicology1687-82052022-01-01202210.1155/2022/3596768Determination of Mycotoxin Contamination Levels in Rice and Dietary Exposure AssessmentJose Troestch0Stephany Reyes1Aracelly Vega2Centro de Investigación en Recursos NaturalesCentro de Investigación en Recursos NaturalesCentro de Investigación en Recursos NaturalesThe contamination by aflatoxins, ochratoxin A, and zearalenone of samples of paddy and polished rice stored in silos located in Chiriquí, Panama, was evaluated. A total of 23 samples were extracted using immunoaffinity columns and analyzed by high-performance liquid chromatography (HPLC) with a fluorescence detector (FLD) and post-column photochemical derivatization. For the method used, the detection limits were lower than 0.25 μg/Kg for aflatoxins (AFB1, AFB2, AFG1, AFG2) and ochratoxin A and 9.35 μg/Kg for zearalenone; the limits of quantification were between 0.25 and 18.75 μg/Kg, respectively. Of the samples analyzed, all of the paddy rice samples were positive for at least one of the mycotoxins studied, zearalenone being the one found with the highest incidence (90.91%); for the polished rice samples, the mycotoxin with the highest incidence was zearalenone (50%), although in concentrations lower than those established in European legislation (100 μg/Kg). The estimate of the daily zearalenone intake according to the concentrations found was always less than 0.07 μg/Kg/bw. This is the first report on the determination of 6 mycotoxins in rice grains from Panama by the HPLC-FLD methodology. Considering the high incidence of mycotoxins in the analyzed rice samples, regular control in the production process is recommended to improve quality and ascertain its safety.http://dx.doi.org/10.1155/2022/3596768 |
| spellingShingle | Jose Troestch Stephany Reyes Aracelly Vega Determination of Mycotoxin Contamination Levels in Rice and Dietary Exposure Assessment Journal of Toxicology |
| title | Determination of Mycotoxin Contamination Levels in Rice and Dietary Exposure Assessment |
| title_full | Determination of Mycotoxin Contamination Levels in Rice and Dietary Exposure Assessment |
| title_fullStr | Determination of Mycotoxin Contamination Levels in Rice and Dietary Exposure Assessment |
| title_full_unstemmed | Determination of Mycotoxin Contamination Levels in Rice and Dietary Exposure Assessment |
| title_short | Determination of Mycotoxin Contamination Levels in Rice and Dietary Exposure Assessment |
| title_sort | determination of mycotoxin contamination levels in rice and dietary exposure assessment |
| url | http://dx.doi.org/10.1155/2022/3596768 |
| work_keys_str_mv | AT josetroestch determinationofmycotoxincontaminationlevelsinriceanddietaryexposureassessment AT stephanyreyes determinationofmycotoxincontaminationlevelsinriceanddietaryexposureassessment AT aracellyvega determinationofmycotoxincontaminationlevelsinriceanddietaryexposureassessment |