Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation
Paracoccidioidomycosis (PCM) is a systemic fungal infection, endemic in Brazil, that leads to severe morbidity and even mortality if not correctly treated. Patients may respond differently to PCM depending on the pattern of the acquired immune response developed. The onset of protective immune respo...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/430525 |
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| author | Felipe Fornias Sperandio Gisele Pesquero Fernandes Ana Carolina Silvério Cerqueira Mendes Giulia Maria de Alencar Castro Bani Vera Lucia Garcia Calich Eva Burger |
| author_facet | Felipe Fornias Sperandio Gisele Pesquero Fernandes Ana Carolina Silvério Cerqueira Mendes Giulia Maria de Alencar Castro Bani Vera Lucia Garcia Calich Eva Burger |
| author_sort | Felipe Fornias Sperandio |
| collection | DOAJ |
| description | Paracoccidioidomycosis (PCM) is a systemic fungal infection, endemic in Brazil, that leads to severe morbidity and even mortality if not correctly treated. Patients may respond differently to PCM depending on the pattern of the acquired immune response developed. The onset of protective immune response is notably mediated by neutrophils (PMN) that play an important role through directly killing the fungi and also by interacting with other cell types to modulate the acquired protective immune response that may follow. In that way, this study aimed to present and compare different experimental models of PCM (intraperitoneal and subcutaneous) regarding PMN production and maturation inside femoral bone marrow and also PMN infiltration in peritoneal and subcutaneous exudates of resistant and susceptible mice. We also assessed the fungal colony forming units and the levels of soluble inflammatory mediators (LTB4, KC, IFN-γ, GM-CSF, and IL-10) inside subcutaneous air-pouches to compare the efficiency of the PMN present at this site in relation to the two main neutrophil functions: initial lysis of the invading pathogen and modulation of the acquired immune response. P. brasiliensis inoculated intraperitoneally was able to disseminate to the bone marrow of susceptible mice, causing a more marked alteration of PMN production and maturation than that observed after resistant mice infection by the same route. Subcutaneous air-pouch inoculation of P. brasiliensis elicited a controlled and limited infection that produced a PMN-rich exudate, thus favoring the study of the interaction between the fungus and the neutrophils. Susceptible mice produced higher numbers of PMN; however, these cells were less effective in killing the fungi. Inflammatory cytokines were more pronounced in resistant mice, which supports their PCM raised resistance. |
| format | Article |
| id | doaj-art-71dcc5da50b844e5a8a4fe360c0d816d |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-71dcc5da50b844e5a8a4fe360c0d816d2025-02-03T05:45:13ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/430525430525Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of InflammationFelipe Fornias Sperandio0Gisele Pesquero Fernandes1Ana Carolina Silvério Cerqueira Mendes2Giulia Maria de Alencar Castro Bani3Vera Lucia Garcia Calich4Eva Burger5Instituto de Ciências Biomédicas, Universidade Federal de Alfenas (UNIFAL), BrazilSetor de Ciências da Saúde, Universidade Federal do Paraná (UFPR), BrazilInstituto de Ciências Biomédicas, Universidade Federal de Alfenas (UNIFAL), BrazilInstituto de Ciências Biomédicas, Universidade Federal de Alfenas (UNIFAL), BrazilInstituto de Ciências Biomédicas, Universidade de São Paulo (USP), BrazilInstituto de Ciências Biomédicas, Universidade Federal de Alfenas (UNIFAL), BrazilParacoccidioidomycosis (PCM) is a systemic fungal infection, endemic in Brazil, that leads to severe morbidity and even mortality if not correctly treated. Patients may respond differently to PCM depending on the pattern of the acquired immune response developed. The onset of protective immune response is notably mediated by neutrophils (PMN) that play an important role through directly killing the fungi and also by interacting with other cell types to modulate the acquired protective immune response that may follow. In that way, this study aimed to present and compare different experimental models of PCM (intraperitoneal and subcutaneous) regarding PMN production and maturation inside femoral bone marrow and also PMN infiltration in peritoneal and subcutaneous exudates of resistant and susceptible mice. We also assessed the fungal colony forming units and the levels of soluble inflammatory mediators (LTB4, KC, IFN-γ, GM-CSF, and IL-10) inside subcutaneous air-pouches to compare the efficiency of the PMN present at this site in relation to the two main neutrophil functions: initial lysis of the invading pathogen and modulation of the acquired immune response. P. brasiliensis inoculated intraperitoneally was able to disseminate to the bone marrow of susceptible mice, causing a more marked alteration of PMN production and maturation than that observed after resistant mice infection by the same route. Subcutaneous air-pouch inoculation of P. brasiliensis elicited a controlled and limited infection that produced a PMN-rich exudate, thus favoring the study of the interaction between the fungus and the neutrophils. Susceptible mice produced higher numbers of PMN; however, these cells were less effective in killing the fungi. Inflammatory cytokines were more pronounced in resistant mice, which supports their PCM raised resistance.http://dx.doi.org/10.1155/2015/430525 |
| spellingShingle | Felipe Fornias Sperandio Gisele Pesquero Fernandes Ana Carolina Silvério Cerqueira Mendes Giulia Maria de Alencar Castro Bani Vera Lucia Garcia Calich Eva Burger Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation Mediators of Inflammation |
| title | Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation |
| title_full | Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation |
| title_fullStr | Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation |
| title_full_unstemmed | Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation |
| title_short | Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation |
| title_sort | resistance to p brasiliensis experimental infection of inbred mice is associated with an efficient neutrophil mobilization and activation by mediators of inflammation |
| url | http://dx.doi.org/10.1155/2015/430525 |
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