Nonylphenol promotes epithelial-mesenchymal transition in colorectal cancer cells by upregulating miR-151a-3p
Abstract Nonylphenol (NP) is a common environmental contaminant and endocrine disruptor. Our previous research demonstrated that NP could promote the proliferation and epithelial-mesenchymal transition (EMT) of colorectal cancer (CRC) cells; however, the specific mechanism remains unclear. miRNA seq...
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| Format: | Article |
| Language: | English |
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Springer
2025-01-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-025-01805-y |
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| author | Biao Wang Nianjie Zhang Lin Dai Yuanwei Zhang Shuo Yin Xuefeng Yang |
| author_facet | Biao Wang Nianjie Zhang Lin Dai Yuanwei Zhang Shuo Yin Xuefeng Yang |
| author_sort | Biao Wang |
| collection | DOAJ |
| description | Abstract Nonylphenol (NP) is a common environmental contaminant and endocrine disruptor. Our previous research demonstrated that NP could promote the proliferation and epithelial-mesenchymal transition (EMT) of colorectal cancer (CRC) cells; however, the specific mechanism remains unclear. miRNA sequencing revealed that NP upregulated the expression levels of microRNA(miR)-151a-3p in CRC. Analysis of The Cancer Genome Atlas (TCGA) data revealed increased expression levels of miR-151a-3p in CRC tissues. The present experiments showed that NP could activate the WNT/β-catenin signaling pathway, and promoted the migration and invasion of CRC cells by increasing the expression levels of miR-151a-3p. Through bioinformatics analysis and dual-luciferase reporter assays, Fyn-related kinase (FRK) was identified as a target gene of miR-151a-3p. Knockdown of FRK promoted NP-induced EMT in CRC cells and activated the WNT/β-catenin signaling pathway, while overexpression had the opposite effect. In summary, the present study demonstrated that NP could inhibit FRK expression via miR-151a-3p, activate the WNT/β-catenin signaling pathway, and promote EMT in CRC cells. |
| format | Article |
| id | doaj-art-7173e9176f4543a7a7b6f07319d8a1da |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-7173e9176f4543a7a7b6f07319d8a1da2025-01-26T12:39:44ZengSpringerDiscover Oncology2730-60112025-01-0116111610.1007/s12672-025-01805-yNonylphenol promotes epithelial-mesenchymal transition in colorectal cancer cells by upregulating miR-151a-3pBiao Wang0Nianjie Zhang1Lin Dai2Yuanwei Zhang3Shuo Yin4Xuefeng Yang5Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Zunyi Medical UniversityDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Zunyi Medical UniversityDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Zunyi Medical UniversityDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Zunyi Medical UniversityDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Zunyi Medical UniversityDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Zunyi Medical UniversityAbstract Nonylphenol (NP) is a common environmental contaminant and endocrine disruptor. Our previous research demonstrated that NP could promote the proliferation and epithelial-mesenchymal transition (EMT) of colorectal cancer (CRC) cells; however, the specific mechanism remains unclear. miRNA sequencing revealed that NP upregulated the expression levels of microRNA(miR)-151a-3p in CRC. Analysis of The Cancer Genome Atlas (TCGA) data revealed increased expression levels of miR-151a-3p in CRC tissues. The present experiments showed that NP could activate the WNT/β-catenin signaling pathway, and promoted the migration and invasion of CRC cells by increasing the expression levels of miR-151a-3p. Through bioinformatics analysis and dual-luciferase reporter assays, Fyn-related kinase (FRK) was identified as a target gene of miR-151a-3p. Knockdown of FRK promoted NP-induced EMT in CRC cells and activated the WNT/β-catenin signaling pathway, while overexpression had the opposite effect. In summary, the present study demonstrated that NP could inhibit FRK expression via miR-151a-3p, activate the WNT/β-catenin signaling pathway, and promote EMT in CRC cells.https://doi.org/10.1007/s12672-025-01805-yNonylphenolColorectal cancermicroRNA-151a-3pFyn-related kinaseWNT/β-cateninEpithelial-mesenchymal transition |
| spellingShingle | Biao Wang Nianjie Zhang Lin Dai Yuanwei Zhang Shuo Yin Xuefeng Yang Nonylphenol promotes epithelial-mesenchymal transition in colorectal cancer cells by upregulating miR-151a-3p Discover Oncology Nonylphenol Colorectal cancer microRNA-151a-3p Fyn-related kinase WNT/β-catenin Epithelial-mesenchymal transition |
| title | Nonylphenol promotes epithelial-mesenchymal transition in colorectal cancer cells by upregulating miR-151a-3p |
| title_full | Nonylphenol promotes epithelial-mesenchymal transition in colorectal cancer cells by upregulating miR-151a-3p |
| title_fullStr | Nonylphenol promotes epithelial-mesenchymal transition in colorectal cancer cells by upregulating miR-151a-3p |
| title_full_unstemmed | Nonylphenol promotes epithelial-mesenchymal transition in colorectal cancer cells by upregulating miR-151a-3p |
| title_short | Nonylphenol promotes epithelial-mesenchymal transition in colorectal cancer cells by upregulating miR-151a-3p |
| title_sort | nonylphenol promotes epithelial mesenchymal transition in colorectal cancer cells by upregulating mir 151a 3p |
| topic | Nonylphenol Colorectal cancer microRNA-151a-3p Fyn-related kinase WNT/β-catenin Epithelial-mesenchymal transition |
| url | https://doi.org/10.1007/s12672-025-01805-y |
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