Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011
Methane monooxygenase (MMO) has been found in methanotrophic bacteria, which catalyzes the epoxidation of gaseous alkenes to their corresponding epoxides. The whole cell suspension of Methylosinus trichosporium IMV 3011 was used to produce epoxyethane from ethylene. The optimal reaction time and ini...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/9191382 |
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| author | Jia-Ying Xin Ning Xu Sheng-Fu Ji Yan Wang Chun-Gu Xia |
| author_facet | Jia-Ying Xin Ning Xu Sheng-Fu Ji Yan Wang Chun-Gu Xia |
| author_sort | Jia-Ying Xin |
| collection | DOAJ |
| description | Methane monooxygenase (MMO) has been found in methanotrophic bacteria, which catalyzes the epoxidation of gaseous alkenes to their corresponding epoxides. The whole cell suspension of Methylosinus trichosporium IMV 3011 was used to produce epoxyethane from ethylene. The optimal reaction time and initial ethylene concentration for ethylene epoxidation have been described. The product epoxyethane is not further metabolized and accumulates extracellularly. Thus, exhaustion of reductant and the inhibition of toxic products make it difficult to accumulate epoxyethane continuously. In order to settle these problems, regeneration of cofactor NADH was performed in batch experiments with methane and methanol. The amount of epoxyethane formed before cosubstrate regeneration was between 0.8 and 1.0 nmol/50 mg cells in approximately 8 h. Combining data from 7 batch experiments, the total production of epoxyethane was 2.2 nmol. Production of epoxyethane was improved (4.6 nmol) in 10% gas phase methane since methane acts as an abundant reductant for epoxidation. It was found that the maximum production of epoxyethane (6.6 nmol) occurs with 3 mmol/L methanol. The passive effect of epoxyethane accumulation on epoxyethane production capacity of Methylosinus trichosporium IMV 3011 in batch experiments was studied. Removal of product was suggested to overcome the inhibition of epoxyethane production. |
| format | Article |
| id | doaj-art-7167ae4d47754b0aa4be469949fdce15 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-7167ae4d47754b0aa4be469949fdce152025-02-03T06:44:26ZengWileyJournal of Chemistry2090-90632090-90712017-01-01201710.1155/2017/91913829191382Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011Jia-Ying Xin0Ning Xu1Sheng-Fu Ji2Yan Wang3Chun-Gu Xia4Key Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076, ChinaKey Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076, ChinaState Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, ChinaKey Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076, ChinaState Key Laboratory for Oxo Synthesis & Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, ChinaMethane monooxygenase (MMO) has been found in methanotrophic bacteria, which catalyzes the epoxidation of gaseous alkenes to their corresponding epoxides. The whole cell suspension of Methylosinus trichosporium IMV 3011 was used to produce epoxyethane from ethylene. The optimal reaction time and initial ethylene concentration for ethylene epoxidation have been described. The product epoxyethane is not further metabolized and accumulates extracellularly. Thus, exhaustion of reductant and the inhibition of toxic products make it difficult to accumulate epoxyethane continuously. In order to settle these problems, regeneration of cofactor NADH was performed in batch experiments with methane and methanol. The amount of epoxyethane formed before cosubstrate regeneration was between 0.8 and 1.0 nmol/50 mg cells in approximately 8 h. Combining data from 7 batch experiments, the total production of epoxyethane was 2.2 nmol. Production of epoxyethane was improved (4.6 nmol) in 10% gas phase methane since methane acts as an abundant reductant for epoxidation. It was found that the maximum production of epoxyethane (6.6 nmol) occurs with 3 mmol/L methanol. The passive effect of epoxyethane accumulation on epoxyethane production capacity of Methylosinus trichosporium IMV 3011 in batch experiments was studied. Removal of product was suggested to overcome the inhibition of epoxyethane production.http://dx.doi.org/10.1155/2017/9191382 |
| spellingShingle | Jia-Ying Xin Ning Xu Sheng-Fu Ji Yan Wang Chun-Gu Xia Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011 Journal of Chemistry |
| title | Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011 |
| title_full | Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011 |
| title_fullStr | Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011 |
| title_full_unstemmed | Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011 |
| title_short | Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011 |
| title_sort | epoxidation of ethylene by whole cell suspension of methylosinus trichosporium imv 3011 |
| url | http://dx.doi.org/10.1155/2017/9191382 |
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