MAGI2-AS3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via miRNA-31-5p/AR axis
Molecular regulatory mechanism of MAGI2-AS3 in HNSCC is not yet mature.In this study, we analyzed the methylation level of MAGI2-AS3 promoter and its downstream miR-31-5p/AR axis by bioinformatics methods. qRT-PCR was used to detect the mRNA expression level of each gene, and western blot was used t...
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Elsevier
2025-02-01
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| Series: | Translational Oncology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523324003498 |
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| author | Kai Yue Ting Zhang Huanhuan Wang Bo Wang Yalin Mu Hui Li |
| author_facet | Kai Yue Ting Zhang Huanhuan Wang Bo Wang Yalin Mu Hui Li |
| author_sort | Kai Yue |
| collection | DOAJ |
| description | Molecular regulatory mechanism of MAGI2-AS3 in HNSCC is not yet mature.In this study, we analyzed the methylation level of MAGI2-AS3 promoter and its downstream miR-31-5p/AR axis by bioinformatics methods. qRT-PCR was used to detect the mRNA expression level of each gene, and western blot was used to detect the expression level of AR proteins in tissues and cells. CCK-8, colony formation, wound healing, and cellular invasion assays were used to detect the HNSCC cell proliferation, migration, and invasion. Dual luciferase and RIP assays were performed to validate the binding relationship between genes. The effect of MAGI2-AS3 on HNSCC progression was verified in nude mice in vivo.The low expression of MAGI2-AS3 in HNSCC was caused by hypermethylation of MAGI2-AS3, which could regulate the target of miR-31-5p by sponge adsorption of miR-31-5p, and miR-31-5p could inhibit the expression of AR by directly targeting AR. Thus, MAGI2-AS3 could inhibit the proliferation, migration, and invasion of HNSCC through the miR-31-5p/AR axis. This provided a theoretical basis that MAGI2-AS3 was a potential therapeutic target for HNSCC. |
| format | Article |
| id | doaj-art-71565ed5d4ac46b8a1488b1ef33f9351 |
| institution | Kabale University |
| issn | 1936-5233 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj-art-71565ed5d4ac46b8a1488b1ef33f93512025-01-22T05:41:23ZengElsevierTranslational Oncology1936-52332025-02-0152102223MAGI2-AS3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via miRNA-31-5p/AR axisKai Yue0Ting Zhang1Huanhuan Wang2Bo Wang3Yalin Mu4Hui Li5Department of Oncology, Nanyang Central Hospital, Nanyang 473005, ChinaDepartment of Oncology, Nanyang Central Hospital, Nanyang 473005, ChinaDepartment of Oncology, Nanyang Central Hospital, Nanyang 473005, ChinaDepartment of Oncology, Nanyang Central Hospital, Nanyang 473005, ChinaDepartment of Oncology, Nanyang Central Hospital, Nanyang 473005, ChinaDepartment of Scientific Research, Nanyang Central Hospital, Nanyang 473005, China; Corresponding author at: Scientific Research Department, Nanyang Central Hospital, No. 312 Gongnong Road, Wancheng District, Nanyang City, Henan Province 473005, China.Molecular regulatory mechanism of MAGI2-AS3 in HNSCC is not yet mature.In this study, we analyzed the methylation level of MAGI2-AS3 promoter and its downstream miR-31-5p/AR axis by bioinformatics methods. qRT-PCR was used to detect the mRNA expression level of each gene, and western blot was used to detect the expression level of AR proteins in tissues and cells. CCK-8, colony formation, wound healing, and cellular invasion assays were used to detect the HNSCC cell proliferation, migration, and invasion. Dual luciferase and RIP assays were performed to validate the binding relationship between genes. The effect of MAGI2-AS3 on HNSCC progression was verified in nude mice in vivo.The low expression of MAGI2-AS3 in HNSCC was caused by hypermethylation of MAGI2-AS3, which could regulate the target of miR-31-5p by sponge adsorption of miR-31-5p, and miR-31-5p could inhibit the expression of AR by directly targeting AR. Thus, MAGI2-AS3 could inhibit the proliferation, migration, and invasion of HNSCC through the miR-31-5p/AR axis. This provided a theoretical basis that MAGI2-AS3 was a potential therapeutic target for HNSCC.http://www.sciencedirect.com/science/article/pii/S1936523324003498MethylationMAGI2-AS3miRNA-31-5p/AR axisHNSCCMigrationInvasion |
| spellingShingle | Kai Yue Ting Zhang Huanhuan Wang Bo Wang Yalin Mu Hui Li MAGI2-AS3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via miRNA-31-5p/AR axis Translational Oncology Methylation MAGI2-AS3 miRNA-31-5p/AR axis HNSCC Migration Invasion |
| title | MAGI2-AS3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via miRNA-31-5p/AR axis |
| title_full | MAGI2-AS3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via miRNA-31-5p/AR axis |
| title_fullStr | MAGI2-AS3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via miRNA-31-5p/AR axis |
| title_full_unstemmed | MAGI2-AS3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via miRNA-31-5p/AR axis |
| title_short | MAGI2-AS3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via miRNA-31-5p/AR axis |
| title_sort | magi2 as3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via mirna 31 5p ar axis |
| topic | Methylation MAGI2-AS3 miRNA-31-5p/AR axis HNSCC Migration Invasion |
| url | http://www.sciencedirect.com/science/article/pii/S1936523324003498 |
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