Exploration of Some Nutrient Absorption–Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan
ABSTRACT Background Quail is an efficient poultry species known for many years, and its productivity is highly affected by gastrointestinal function, health, digestion and commensal bacteria. Specific histological parameters and nutrient absorption–related genes could be used to study intestinal fun...
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Wiley
2024-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70056 |
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| author | Hamid Reza Rafieian‐Naeini Mahdi Zhandi Maryam Taghipour‐Shahbandi Amir Reza Sabzian Ali Reza Yousefi Mahboubeh Khajehnabi Woo Kyun Kim |
| author_facet | Hamid Reza Rafieian‐Naeini Mahdi Zhandi Maryam Taghipour‐Shahbandi Amir Reza Sabzian Ali Reza Yousefi Mahboubeh Khajehnabi Woo Kyun Kim |
| author_sort | Hamid Reza Rafieian‐Naeini |
| collection | DOAJ |
| description | ABSTRACT Background Quail is an efficient poultry species known for many years, and its productivity is highly affected by gastrointestinal function, health, digestion and commensal bacteria. Specific histological parameters and nutrient absorption–related genes could be used to study intestinal functionality and efficiency. Objectives This study aimed to evaluate the mRNA abundance of nutrient absorption–related genes and intestinal histology changes in laying Japanese quail during productive lifespan. Methods A total of 135 quails from a commercial flock at different egg production periods (early production [EP, 6 weeks of age], pick production [PP, 21 weeks of age] and post pick production [PPP, 40 weeks of age]) were randomly selected (n = 45/group) and kept for 6 weeks. Production performance was evaluated during last 2 weeks of the experimental period, and tissue samples were taken from the duodenum, jejunum and ileum for histological analysis and mRNA abundance purposes at the end of the experiment. The mRNA abundance of the CALB1, SGLT1, SGLT5, EAAT3 and NaPi‐IIb genes were compared among groups. Results Production period did not affect the eggshell thickness, goblet cell number and mRNA abundance of CALB1, EAAT3 and NaPi‐IIb (p > 0.05). The PPP quails had the highest egg weight and FCR compared to EP and PP quails (p < 0.001). Egg production and egg mass of the EP and PP quails were higher than those of PPP quails (p < 0.001). Moreover, EP quails had the highest villus height (VH)/crypt depth (CD) ratio in the duodenum and jejunum and the lowest VH:CD in the ileum compared with the PPP quails (p < 0.01). The lowest SGLT1 mRNA abundance was noted in the PP quails, whereas EP quails had the highest SGLT5 mRNA abundance (p < 0.05). Conclusions In conclusion, although histology parameter changes were in‐line with the lowest performance observed in the PPP quails, the current results failed to show a relationship between mRNA abundance changes and production performance attributes at different productive lifespans. |
| format | Article |
| id | doaj-art-71432738dc194b65bab979f506293f33 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-71432738dc194b65bab979f506293f332025-01-20T17:16:45ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70056Exploration of Some Nutrient Absorption–Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive LifespanHamid Reza Rafieian‐Naeini0Mahdi Zhandi1Maryam Taghipour‐Shahbandi2Amir Reza Sabzian3Ali Reza Yousefi4Mahboubeh Khajehnabi5Woo Kyun Kim6Department of Poultry Science University of Georgia Athens Georgia USADepartment of Animal Science College of Agriculture and Natural Resources University of Tehran Karaj Alborz IranDepartment of Animal Science College of Agriculture and Natural Resources University of Tehran Karaj Alborz IranBiomedical Science College of Medicine Ohio State University Columbus Ohio USADepartment of Pathology and Experimental Animals Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO) Karaj Alborz IranChaltasian Agri‐Animal Production Complex Varamin IranDepartment of Poultry Science University of Georgia Athens Georgia USAABSTRACT Background Quail is an efficient poultry species known for many years, and its productivity is highly affected by gastrointestinal function, health, digestion and commensal bacteria. Specific histological parameters and nutrient absorption–related genes could be used to study intestinal functionality and efficiency. Objectives This study aimed to evaluate the mRNA abundance of nutrient absorption–related genes and intestinal histology changes in laying Japanese quail during productive lifespan. Methods A total of 135 quails from a commercial flock at different egg production periods (early production [EP, 6 weeks of age], pick production [PP, 21 weeks of age] and post pick production [PPP, 40 weeks of age]) were randomly selected (n = 45/group) and kept for 6 weeks. Production performance was evaluated during last 2 weeks of the experimental period, and tissue samples were taken from the duodenum, jejunum and ileum for histological analysis and mRNA abundance purposes at the end of the experiment. The mRNA abundance of the CALB1, SGLT1, SGLT5, EAAT3 and NaPi‐IIb genes were compared among groups. Results Production period did not affect the eggshell thickness, goblet cell number and mRNA abundance of CALB1, EAAT3 and NaPi‐IIb (p > 0.05). The PPP quails had the highest egg weight and FCR compared to EP and PP quails (p < 0.001). Egg production and egg mass of the EP and PP quails were higher than those of PPP quails (p < 0.001). Moreover, EP quails had the highest villus height (VH)/crypt depth (CD) ratio in the duodenum and jejunum and the lowest VH:CD in the ileum compared with the PPP quails (p < 0.01). The lowest SGLT1 mRNA abundance was noted in the PP quails, whereas EP quails had the highest SGLT5 mRNA abundance (p < 0.05). Conclusions In conclusion, although histology parameter changes were in‐line with the lowest performance observed in the PPP quails, the current results failed to show a relationship between mRNA abundance changes and production performance attributes at different productive lifespans.https://doi.org/10.1002/vms3.70056gene expressionhistologyproductionquail |
| spellingShingle | Hamid Reza Rafieian‐Naeini Mahdi Zhandi Maryam Taghipour‐Shahbandi Amir Reza Sabzian Ali Reza Yousefi Mahboubeh Khajehnabi Woo Kyun Kim Exploration of Some Nutrient Absorption–Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan Veterinary Medicine and Science gene expression histology production quail |
| title | Exploration of Some Nutrient Absorption–Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan |
| title_full | Exploration of Some Nutrient Absorption–Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan |
| title_fullStr | Exploration of Some Nutrient Absorption–Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan |
| title_full_unstemmed | Exploration of Some Nutrient Absorption–Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan |
| title_short | Exploration of Some Nutrient Absorption–Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan |
| title_sort | exploration of some nutrient absorption related genes and intestinal histology in japanese quails coturnix japonica throughout their productive lifespan |
| topic | gene expression histology production quail |
| url | https://doi.org/10.1002/vms3.70056 |
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