Application of Intracellular Alkaline Phosphatase Activity Measurement in Detection of Neutrophil Adherence In Vitro
We have proposed the use of the fluorimetric method with 4-methylumbelliferyl phosphate (4-MUP) specific substrate for the alkaline phosphatase determination in the neutrophil adhesion assay. We provide evidence that the endogenous neutrophil alkaline phosphatase (NAP) activity evaluation is reliabl...
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/19307 |
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| _version_ | 1832566544185425920 |
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| author | Katarzyna Bednarska Magdalena Klink Zofia Sulowska |
| author_facet | Katarzyna Bednarska Magdalena Klink Zofia Sulowska |
| author_sort | Katarzyna Bednarska |
| collection | DOAJ |
| description | We have proposed the use of the fluorimetric method with 4-methylumbelliferyl
phosphate (4-MUP) specific substrate for the alkaline phosphatase determination in the
neutrophil adhesion assay. We provide evidence that the endogenous neutrophil alkaline
phosphatase (NAP) activity evaluation is reliable to quantify neutrophil adhesion at a wide range of cell numbers (104–106). The results obtained by fluorimetric NAP activity test
correlate to the results of adherence evaluated using the MTT reduction assay. The
fluorimetric NAP activity test may be applied for resting as well as activated neutrophils
without the risk of the activators interferences into the test. The alkaline phosphatase survey
with the use of 4-MUP substrate is recommended herein as a sensitive, repeatable, simple, and
reliable method of the neutrophil adherence determination in vitro. |
| format | Article |
| id | doaj-art-7137a7d51583426b9b8e8310b4c0d723 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7137a7d51583426b9b8e8310b4c0d7232025-02-03T01:03:44ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/1930719307Application of Intracellular Alkaline Phosphatase Activity Measurement in Detection of Neutrophil Adherence In VitroKatarzyna Bednarska0Magdalena Klink1Zofia Sulowska2Department of Experimental Immunology, Centre for Medical Biology, Polish Academy of Sciences, Lodowa 106, Lodz 93-232, PolandDepartment of Experimental Immunology, Centre for Medical Biology, Polish Academy of Sciences, Lodowa 106, Lodz 93-232, PolandDepartment of Experimental Immunology, Centre for Medical Biology, Polish Academy of Sciences, Lodowa 106, Lodz 93-232, PolandWe have proposed the use of the fluorimetric method with 4-methylumbelliferyl phosphate (4-MUP) specific substrate for the alkaline phosphatase determination in the neutrophil adhesion assay. We provide evidence that the endogenous neutrophil alkaline phosphatase (NAP) activity evaluation is reliable to quantify neutrophil adhesion at a wide range of cell numbers (104–106). The results obtained by fluorimetric NAP activity test correlate to the results of adherence evaluated using the MTT reduction assay. The fluorimetric NAP activity test may be applied for resting as well as activated neutrophils without the risk of the activators interferences into the test. The alkaline phosphatase survey with the use of 4-MUP substrate is recommended herein as a sensitive, repeatable, simple, and reliable method of the neutrophil adherence determination in vitro.http://dx.doi.org/10.1155/MI/2006/19307 |
| spellingShingle | Katarzyna Bednarska Magdalena Klink Zofia Sulowska Application of Intracellular Alkaline Phosphatase Activity Measurement in Detection of Neutrophil Adherence In Vitro Mediators of Inflammation |
| title | Application of Intracellular Alkaline Phosphatase Activity Measurement in Detection of Neutrophil Adherence In Vitro |
| title_full | Application of Intracellular Alkaline Phosphatase Activity Measurement in Detection of Neutrophil Adherence In Vitro |
| title_fullStr | Application of Intracellular Alkaline Phosphatase Activity Measurement in Detection of Neutrophil Adherence In Vitro |
| title_full_unstemmed | Application of Intracellular Alkaline Phosphatase Activity Measurement in Detection of Neutrophil Adherence In Vitro |
| title_short | Application of Intracellular Alkaline Phosphatase Activity Measurement in Detection of Neutrophil Adherence In Vitro |
| title_sort | application of intracellular alkaline phosphatase activity measurement in detection of neutrophil adherence in vitro |
| url | http://dx.doi.org/10.1155/MI/2006/19307 |
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