Application of Intracellular Alkaline Phosphatase Activity Measurement in Detection of Neutrophil Adherence In Vitro
We have proposed the use of the fluorimetric method with 4-methylumbelliferyl phosphate (4-MUP) specific substrate for the alkaline phosphatase determination in the neutrophil adhesion assay. We provide evidence that the endogenous neutrophil alkaline phosphatase (NAP) activity evaluation is reliabl...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2006-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/19307 |
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| Summary: | We have proposed the use of the fluorimetric method with 4-methylumbelliferyl
phosphate (4-MUP) specific substrate for the alkaline phosphatase determination in the
neutrophil adhesion assay. We provide evidence that the endogenous neutrophil alkaline
phosphatase (NAP) activity evaluation is reliable to quantify neutrophil adhesion at a wide range of cell numbers (104–106). The results obtained by fluorimetric NAP activity test
correlate to the results of adherence evaluated using the MTT reduction assay. The
fluorimetric NAP activity test may be applied for resting as well as activated neutrophils
without the risk of the activators interferences into the test. The alkaline phosphatase survey
with the use of 4-MUP substrate is recommended herein as a sensitive, repeatable, simple, and
reliable method of the neutrophil adherence determination in vitro. |
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| ISSN: | 0962-9351 1466-1861 |