Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application
We present a new biosensor for hydrogen peroxide (H2O2) detection. The biosensor was based on the immobilization of horseradish peroxidase (HRP) enzyme on layered double hydroxides- (LDH-) modified gold surface. The hydrotalcite LDH (Mg2Al) was prepared by coprecipitation in constant pH and in ambie...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Electrochemistry |
| Online Access: | http://dx.doi.org/10.4061/2011/934893 |
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| author | Zouhair M. Baccar Imène Hafaiedh |
| author_facet | Zouhair M. Baccar Imène Hafaiedh |
| author_sort | Zouhair M. Baccar |
| collection | DOAJ |
| description | We present a new biosensor for hydrogen peroxide (H2O2) detection. The biosensor was based on the immobilization of horseradish peroxidase (HRP) enzyme on layered double hydroxides- (LDH-) modified gold surface. The hydrotalcite LDH (Mg2Al) was prepared by coprecipitation in constant pH and in ambient temperature. The immobilization of the peroxidase on layered hybrid materials was realized via electrostatic adsorption autoassembly process. The detection of hydrogen peroxide was successfully observed in PBS buffer with cyclic voltammetry and the chronoamperometry techniques. A limit detection of 9 μM of H2O2 was obtained with a good reproducibility. We investigate the sensitivity of our developed biosensor for H2O2 detection in raw milk. |
| format | Article |
| id | doaj-art-712451357e024834b7c6a3797a800684 |
| institution | Kabale University |
| issn | 2090-3537 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Electrochemistry |
| spelling | doaj-art-712451357e024834b7c6a3797a8006842025-02-03T01:10:25ZengWileyInternational Journal of Electrochemistry2090-35372011-01-01201110.4061/2011/934893934893Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor ApplicationZouhair M. Baccar0Imène Hafaiedh1Nanobioengineering Group, National Institute of Research and Physicochemical Analysis (INRAP), Biotechnopôle de Sidi Thabet, Sidi Thabet 2020, TunisiaUnity of Research of Physico-Chemistry of Polymers, IPEST, University of Carthage, BP 57, La Marsa 2075, TunisiaWe present a new biosensor for hydrogen peroxide (H2O2) detection. The biosensor was based on the immobilization of horseradish peroxidase (HRP) enzyme on layered double hydroxides- (LDH-) modified gold surface. The hydrotalcite LDH (Mg2Al) was prepared by coprecipitation in constant pH and in ambient temperature. The immobilization of the peroxidase on layered hybrid materials was realized via electrostatic adsorption autoassembly process. The detection of hydrogen peroxide was successfully observed in PBS buffer with cyclic voltammetry and the chronoamperometry techniques. A limit detection of 9 μM of H2O2 was obtained with a good reproducibility. We investigate the sensitivity of our developed biosensor for H2O2 detection in raw milk.http://dx.doi.org/10.4061/2011/934893 |
| spellingShingle | Zouhair M. Baccar Imène Hafaiedh Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application International Journal of Electrochemistry |
| title | Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application |
| title_full | Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application |
| title_fullStr | Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application |
| title_full_unstemmed | Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application |
| title_short | Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application |
| title_sort | immobilization of hrp enzyme on layered double hydroxides for biosensor application |
| url | http://dx.doi.org/10.4061/2011/934893 |
| work_keys_str_mv | AT zouhairmbaccar immobilizationofhrpenzymeonlayereddoublehydroxidesforbiosensorapplication AT imenehafaiedh immobilizationofhrpenzymeonlayereddoublehydroxidesforbiosensorapplication |