Bacterial Diversity, Chemical Composition, and Fermentation Quality of Alfalfa-Based Total Mixed Ration Silage Inoculated with <i>Lactobacillus reuteri</i> and <i>Lentilactobacillus buchneri</i>
This study examined the effects of lactic acid bacteria (LAB) from extremely cold environments on the bacterial diversity, chemical composition, and fermentation quality of alfalfa-based TMR silage. The TMR was treated with <i>Lactobacillus reuteri</i> A4-2 (Lr A4-2) and <i>Lentila...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
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| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/4/164 |
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| Summary: | This study examined the effects of lactic acid bacteria (LAB) from extremely cold environments on the bacterial diversity, chemical composition, and fermentation quality of alfalfa-based TMR silage. The TMR was treated with <i>Lactobacillus reuteri</i> A4-2 (Lr A4-2) and <i>Lentilactobacillus buchneri</i> 9-2 (Lb 9-2) at an application rate of 1.0 × 10<sup>5</sup> cfu/g fresh material, respectively, and the control received the same volume of distilled water. The TMR was ensiled for 7, 15, 30, 60, and 210 days. The Lr A4-2 treatment produced higher lactic acid (62.53 g/kg DM at 210 days) and maintained a diverse bacterial community throughout the ensiling compared with the control and Lb 9-2 treatment. The Lb 9-2 treatment increased acetic acid (51.42 g/kg DM at 210 days) and formed a distinct bacterial community profile. The 16S rRNA sequencing revealed a shift from initial <i>Weissella</i> dominance to <i>Lactobacillus</i>-dominated communities across the treatments by day 210. Alpha diversity indices decreased over time, with Lr A4-2 treatment maintaining higher diversity. Principal coordinate analysis demonstrated significant temporal shifts in bacterial community composition among treatments (<i>p</i> < 0.01). The results suggest that different heterofermentative LAB strains modulated the fermentation and microbial balance in alfalfa-based TMR silage in different ways. |
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| ISSN: | 2311-5637 |