Interleukin-2 receptor β chain as a possible target for low doses of mafosfamide
The 7-day cytotoxic lymphocytes (CTL) induced in mixed lymphocyte culture express only the chain of the interleukin-2 receptor (IL-2R). In the present study this fact has been confirmed in a murine semi-allogeneic system. The ability of low doses of mafosfamide (Mf) to affect IL-2-induced CTL prolif...
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| Format: | Article |
| Language: | English |
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Wiley
1995-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935195000287 |
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| _version_ | 1832564337596694528 |
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| author | A. Pukhalsky A. Toptygina S. Khaidukov |
| author_facet | A. Pukhalsky A. Toptygina S. Khaidukov |
| author_sort | A. Pukhalsky |
| collection | DOAJ |
| description | The 7-day cytotoxic lymphocytes (CTL) induced in mixed lymphocyte culture express only the chain of the interleukin-2 receptor (IL-2R). In the present study this fact has been confirmed in a murine semi-allogeneic system. The ability of low doses of mafosfamide (Mf) to affect IL-2-induced CTL proliferation has been demonstrated. It was also shown that IL-2 activated resting suppressor cells. The pretreatment of the suppressor cells with either monoclonal antibodies (mAbs) against the p75 chain of IL-2R, or with Mf abolished the suppressive effect of these cells. No restoration of the proliferative response occurred when the anti-IL-2Rα mAb had been used. Flow cytometry analysis of 7-day CTL was carried out with mAbs against the α and β chains of IL-2R. CTL treatment with Mf inhibited anti-IL-2Rβ mAb binding. It may be assumed that the anti-proliferative effects of Mf which have been demonstrated in this paper, were a result of blocking the IL-2R β chain. |
| format | Article |
| id | doaj-art-710b19d0edf94c3fab355b1e5a6c69b3 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-710b19d0edf94c3fab355b1e5a6c69b32025-02-03T01:11:20ZengWileyMediators of Inflammation0962-93511466-18611995-01-014317518010.1155/S0962935195000287Interleukin-2 receptor β chain as a possible target for low doses of mafosfamideA. Pukhalsky0A. Toptygina1S. Khaidukov2Research Centre for Medical Genetics, Moskvorechie St., Moscow 115478, RussiaResearch Centre for Medical Genetics, Moskvorechie St., Moscow 115478, RussiaM.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Moscow, RussiaThe 7-day cytotoxic lymphocytes (CTL) induced in mixed lymphocyte culture express only the chain of the interleukin-2 receptor (IL-2R). In the present study this fact has been confirmed in a murine semi-allogeneic system. The ability of low doses of mafosfamide (Mf) to affect IL-2-induced CTL proliferation has been demonstrated. It was also shown that IL-2 activated resting suppressor cells. The pretreatment of the suppressor cells with either monoclonal antibodies (mAbs) against the p75 chain of IL-2R, or with Mf abolished the suppressive effect of these cells. No restoration of the proliferative response occurred when the anti-IL-2Rα mAb had been used. Flow cytometry analysis of 7-day CTL was carried out with mAbs against the α and β chains of IL-2R. CTL treatment with Mf inhibited anti-IL-2Rβ mAb binding. It may be assumed that the anti-proliferative effects of Mf which have been demonstrated in this paper, were a result of blocking the IL-2R β chain.http://dx.doi.org/10.1155/S0962935195000287 |
| spellingShingle | A. Pukhalsky A. Toptygina S. Khaidukov Interleukin-2 receptor β chain as a possible target for low doses of mafosfamide Mediators of Inflammation |
| title | Interleukin-2 receptor β chain as a possible target for low doses of mafosfamide |
| title_full | Interleukin-2 receptor β chain as a possible target for low doses of mafosfamide |
| title_fullStr | Interleukin-2 receptor β chain as a possible target for low doses of mafosfamide |
| title_full_unstemmed | Interleukin-2 receptor β chain as a possible target for low doses of mafosfamide |
| title_short | Interleukin-2 receptor β chain as a possible target for low doses of mafosfamide |
| title_sort | interleukin 2 receptor β chain as a possible target for low doses of mafosfamide |
| url | http://dx.doi.org/10.1155/S0962935195000287 |
| work_keys_str_mv | AT apukhalsky interleukin2receptorbchainasapossibletargetforlowdosesofmafosfamide AT atoptygina interleukin2receptorbchainasapossibletargetforlowdosesofmafosfamide AT skhaidukov interleukin2receptorbchainasapossibletargetforlowdosesofmafosfamide |