Occurrence of antibiotics and antibiotic resistance genes at various stages of different aquaculture modes surrounding Tai Lake, China
IntroductionAquaculture is an important source of antibiotics and ARGs in environmental waters. However, the occurrence of antibiotics and ARGs under different modes and stages of aquaculture has rarely been systematically studied.MethodsThis paper uses qPCR, LC-MS, and High-Throughput sequencing ac...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1543387/full |
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| author | Congcong Wu Congcong Wu Haitao Ye Mingzhu Xu Xuan Zhao Xuejie Zhao Lina Li Mingzhi Li Yanfei Wei Yuru Li Baolan Hu Baolan Hu |
| author_facet | Congcong Wu Congcong Wu Haitao Ye Mingzhu Xu Xuan Zhao Xuejie Zhao Lina Li Mingzhi Li Yanfei Wei Yuru Li Baolan Hu Baolan Hu |
| author_sort | Congcong Wu |
| collection | DOAJ |
| description | IntroductionAquaculture is an important source of antibiotics and ARGs in environmental waters. However, the occurrence of antibiotics and ARGs under different modes and stages of aquaculture has rarely been systematically studied.MethodsThis paper uses qPCR, LC-MS, and High-Throughput sequencing across different culture modes and stages to investigate antibiotics, resistance genes, and microbial communities in the water bodies, and analyze contamination differences between these modes.ResultsThe quinolone and chloramphenicol were the main antibiotics, and the highest absolute abundance genes were quinolone resistance genes (qnrB) and quinolone resistance genes (sul1), with the mobile genetic element (MGE) intI1, both of which exhibited a gradual seasonal increase. Microbial diversity also varies seasonally, especially with a gradual increase in the abundance of some pathogenic bacteria (Flavobacterium). Antibiotics and resistance genes were found at higher levels in fish ponds compared to shrimp and crab ponds, while they were lower in shrimp and crab ponds that utilized the ecological mode ponds than in the traditional culture mode ponds.ConclusionOur study presents a comprehensive characterization of antibiotics and ARGs in aquaculture waters from various perspectives. Ecological aquaculture modes contribute to reducing antibiotic and resistance gene pollution in water bodies. These findings will support the optimization of aquaculture mode and antibiotic usage to the green and sustainable development of aquaculture finally. |
| format | Article |
| id | doaj-art-7109f1034d1741589971e45acc7e4be1 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-7109f1034d1741589971e45acc7e4be12025-01-31T06:40:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.15433871543387Occurrence of antibiotics and antibiotic resistance genes at various stages of different aquaculture modes surrounding Tai Lake, ChinaCongcong Wu0Congcong Wu1Haitao Ye2Mingzhu Xu3Xuan Zhao4Xuejie Zhao5Lina Li6Mingzhi Li7Yanfei Wei8Yuru Li9Baolan Hu10Baolan Hu11Department of Environmental Engineering, College of Environmental and Resources Sciences, Zhejiang University, Hangzhou, ChinaZhejiang Environmental Technology Co., Ltd., Hangzhou, ChinaZhejiang Qingke Environmental Protection Technology Co., Ltd., Quzhou, ChinaZhejiang Environmental Technology Co., Ltd., Hangzhou, ChinaZhejiang Environmental Technology Co., Ltd., Hangzhou, ChinaZhejiang Environmental Technology Co., Ltd., Hangzhou, ChinaZhejiang Environmental Technology Co., Ltd., Hangzhou, ChinaZhejiang Environmental Technology Co., Ltd., Hangzhou, ChinaZhejiang Environmental Technology Co., Ltd., Hangzhou, ChinaZhejiang Environmental Technology Co., Ltd., Hangzhou, ChinaDepartment of Environmental Engineering, College of Environmental and Resources Sciences, Zhejiang University, Hangzhou, ChinaZhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou, ChinaIntroductionAquaculture is an important source of antibiotics and ARGs in environmental waters. However, the occurrence of antibiotics and ARGs under different modes and stages of aquaculture has rarely been systematically studied.MethodsThis paper uses qPCR, LC-MS, and High-Throughput sequencing across different culture modes and stages to investigate antibiotics, resistance genes, and microbial communities in the water bodies, and analyze contamination differences between these modes.ResultsThe quinolone and chloramphenicol were the main antibiotics, and the highest absolute abundance genes were quinolone resistance genes (qnrB) and quinolone resistance genes (sul1), with the mobile genetic element (MGE) intI1, both of which exhibited a gradual seasonal increase. Microbial diversity also varies seasonally, especially with a gradual increase in the abundance of some pathogenic bacteria (Flavobacterium). Antibiotics and resistance genes were found at higher levels in fish ponds compared to shrimp and crab ponds, while they were lower in shrimp and crab ponds that utilized the ecological mode ponds than in the traditional culture mode ponds.ConclusionOur study presents a comprehensive characterization of antibiotics and ARGs in aquaculture waters from various perspectives. Ecological aquaculture modes contribute to reducing antibiotic and resistance gene pollution in water bodies. These findings will support the optimization of aquaculture mode and antibiotic usage to the green and sustainable development of aquaculture finally.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1543387/fullaquaculture modesantibioticsantibiotic resistance genesmicrobial diversitywater pollution |
| spellingShingle | Congcong Wu Congcong Wu Haitao Ye Mingzhu Xu Xuan Zhao Xuejie Zhao Lina Li Mingzhi Li Yanfei Wei Yuru Li Baolan Hu Baolan Hu Occurrence of antibiotics and antibiotic resistance genes at various stages of different aquaculture modes surrounding Tai Lake, China Frontiers in Microbiology aquaculture modes antibiotics antibiotic resistance genes microbial diversity water pollution |
| title | Occurrence of antibiotics and antibiotic resistance genes at various stages of different aquaculture modes surrounding Tai Lake, China |
| title_full | Occurrence of antibiotics and antibiotic resistance genes at various stages of different aquaculture modes surrounding Tai Lake, China |
| title_fullStr | Occurrence of antibiotics and antibiotic resistance genes at various stages of different aquaculture modes surrounding Tai Lake, China |
| title_full_unstemmed | Occurrence of antibiotics and antibiotic resistance genes at various stages of different aquaculture modes surrounding Tai Lake, China |
| title_short | Occurrence of antibiotics and antibiotic resistance genes at various stages of different aquaculture modes surrounding Tai Lake, China |
| title_sort | occurrence of antibiotics and antibiotic resistance genes at various stages of different aquaculture modes surrounding tai lake china |
| topic | aquaculture modes antibiotics antibiotic resistance genes microbial diversity water pollution |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1543387/full |
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