Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia
A novel alkali-soluble polysaccharide from Lepista sordida (LSAP) mycelia with antioxidative and hepatoprotective activities was characterized. The weight-average molecular weight and number-average molecular weight of LSAP were 1.442 × 103 and 6.05 × 102 kDa, respectively. LSAP was consisted of glu...
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Tsinghua University Press
2021-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302100063X |
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| author | Yingyin Xu Yuanhui Li Yuxiao Lu Xiaobin Feng Guoting Tian Qinghong Liu |
| author_facet | Yingyin Xu Yuanhui Li Yuxiao Lu Xiaobin Feng Guoting Tian Qinghong Liu |
| author_sort | Yingyin Xu |
| collection | DOAJ |
| description | A novel alkali-soluble polysaccharide from Lepista sordida (LSAP) mycelia with antioxidative and hepatoprotective activities was characterized. The weight-average molecular weight and number-average molecular weight of LSAP were 1.442 × 103 and 6.05 × 102 kDa, respectively. LSAP was consisted of glucose (57.9%), xylose (31.8%), and small amounts of rhamnose, arabinose, galactose, glucuronic acid, and galacturonic acid (1.2%–3.1%). The FT-IR and 2D NMR confirmed that LSAP was composed of Xylp, Araf, 4-O-Me-α-D-GlcpA, (1→4)-linked β-D-Glcp, and (1→4)-α-D-GalA, and β-glycosidic linkages between these sugar units. LSAP displayed notable effects on 1,1-dephenyl-2-picryhydrazyl (DPPH) radical scavenging, hydrogen peroxide scavenging, lipid peroxidation inhibitory ability, reducing power and Fe2+ chelating property. These biological effects were further verified by suppressing CCl4-induced oxidative liver damage in mice at doses of 100 and 200 mg/kg. Administration of LSAP in mice prior to CCl4 significantly prevented the CCl4-induced elevation in serum alanine aminotransferase, aspartate aminotransferase, and hepatic malondialdehyde. Mice treated with LSAP demonstrated to increase activities in superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the liver. We also found out that LSAP prevented CCl4-induced oxidative liver histological alteration. LSAP may exert hepatoprotective effects against CCl4-induced damage through antioxidant mechanisms in model mice. |
| format | Article |
| id | doaj-art-70f7cf6df4784159920c17e82d852a2b |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-70f7cf6df4784159920c17e82d852a2b2025-02-03T04:08:20ZengTsinghua University PressFood Science and Human Wellness2213-45302021-07-01104536544Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida myceliaYingyin Xu0Yuanhui Li1Yuxiao Lu2Xiaobin Feng3Guoting Tian4Qinghong Liu5Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaDepartment of Vegetables, College of Horticulture, China Agricultural University, Beijing 100193, ChinaDepartment of Environment and Chemical Engineering, Tangshan College, Tangshan 063000, ChinaDepartment of Vegetables, College of Horticulture, China Agricultural University, Beijing 100193, ChinaInstitute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, ChinaDepartment of Vegetables, College of Horticulture, China Agricultural University, Beijing 100193, China; Corresponding author.A novel alkali-soluble polysaccharide from Lepista sordida (LSAP) mycelia with antioxidative and hepatoprotective activities was characterized. The weight-average molecular weight and number-average molecular weight of LSAP were 1.442 × 103 and 6.05 × 102 kDa, respectively. LSAP was consisted of glucose (57.9%), xylose (31.8%), and small amounts of rhamnose, arabinose, galactose, glucuronic acid, and galacturonic acid (1.2%–3.1%). The FT-IR and 2D NMR confirmed that LSAP was composed of Xylp, Araf, 4-O-Me-α-D-GlcpA, (1→4)-linked β-D-Glcp, and (1→4)-α-D-GalA, and β-glycosidic linkages between these sugar units. LSAP displayed notable effects on 1,1-dephenyl-2-picryhydrazyl (DPPH) radical scavenging, hydrogen peroxide scavenging, lipid peroxidation inhibitory ability, reducing power and Fe2+ chelating property. These biological effects were further verified by suppressing CCl4-induced oxidative liver damage in mice at doses of 100 and 200 mg/kg. Administration of LSAP in mice prior to CCl4 significantly prevented the CCl4-induced elevation in serum alanine aminotransferase, aspartate aminotransferase, and hepatic malondialdehyde. Mice treated with LSAP demonstrated to increase activities in superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the liver. We also found out that LSAP prevented CCl4-induced oxidative liver histological alteration. LSAP may exert hepatoprotective effects against CCl4-induced damage through antioxidant mechanisms in model mice.http://www.sciencedirect.com/science/article/pii/S221345302100063XLepista sordidaPolysaccharideAntioxidant activityHepatoprotective effects |
| spellingShingle | Yingyin Xu Yuanhui Li Yuxiao Lu Xiaobin Feng Guoting Tian Qinghong Liu Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia Food Science and Human Wellness Lepista sordida Polysaccharide Antioxidant activity Hepatoprotective effects |
| title | Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia |
| title_full | Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia |
| title_fullStr | Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia |
| title_full_unstemmed | Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia |
| title_short | Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia |
| title_sort | antioxidative and hepatoprotective activities of a novel polysaccharide lsap from lepista sordida mycelia |
| topic | Lepista sordida Polysaccharide Antioxidant activity Hepatoprotective effects |
| url | http://www.sciencedirect.com/science/article/pii/S221345302100063X |
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