Phenomic characterization of Crotalaria germplasm for crop improvement
Abstract Background Diversification of global food systems through exploration of traditional varieties and wild edible plant species is a focal mitigation strategy for food security worldwide. The present study determined the phenomic diversity of locally available, affordable and climate-resilient...
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CABI
2021-03-01
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| Series: | CABI Agriculture and Bioscience |
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| Online Access: | https://doi.org/10.1186/s43170-021-00031-0 |
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| author | Joshua Kiilu Muli Johnstone O. Neondo Peter K. Kamau Eddy Odari Nancy L. M. Budambula |
| author_facet | Joshua Kiilu Muli Johnstone O. Neondo Peter K. Kamau Eddy Odari Nancy L. M. Budambula |
| author_sort | Joshua Kiilu Muli |
| collection | DOAJ |
| description | Abstract Background Diversification of global food systems through exploration of traditional varieties and wild edible plant species is a focal mitigation strategy for food security worldwide. The present study determined the phenomic diversity of locally available, affordable and climate-resilient cultivated and wild Crotalaria species for breeding purposes. Methods Seed samples were collected from different administrative counties in Kenya spanning different climatic zones. Other seeds were provided by the Genetic Resources Research Institute of Kenya. A randomized complete block design with three replications was used for agro-morphological evaluation of the 83 accessions used in this study. Data on quantitative and qualitative traits was collected. Cluster analysis on R and R-studio was used to generate a dendrogram by the Euclidian genetic distance and dissimilarity indices while the non-metric multidimensional scaling (NMDS) method was used to determine the spatial interrelationship between the accessions. The Pearson’s correlation coefficients were used to determine the relationships between qualitative and quantitative traits while the principal component analysis was used to discriminate the accessions. Results Three edible species (C. brevidens Benth., C. ochroleuca G.Don, C. trichotoma Bojer.) were found to be cultivated by Kenyan farmers and a significant variation (p < 0.0001) for all parameters under study was recorded. Agglomerative hierarchical clustering grouped the accessions into 8 major clusters. The NMDS ordination formed 15 and 6 groups based on counties and regions respectively. Conclusions This study forms the basis for exploring novel breeding strategies among cultivated species, between wild and cultivated Crotalaria species as well as determining molecular markers linked to phenomic traits. |
| format | Article |
| id | doaj-art-70e472a6300146c28eb7f2b663ea2815 |
| institution | Kabale University |
| issn | 2662-4044 |
| language | English |
| publishDate | 2021-03-01 |
| publisher | CABI |
| record_format | Article |
| series | CABI Agriculture and Bioscience |
| spelling | doaj-art-70e472a6300146c28eb7f2b663ea28152025-02-02T19:53:31ZengCABICABI Agriculture and Bioscience2662-40442021-03-012111510.1186/s43170-021-00031-0Phenomic characterization of Crotalaria germplasm for crop improvementJoshua Kiilu Muli0Johnstone O. Neondo1Peter K. Kamau2Eddy Odari3Nancy L. M. Budambula4Department of Biological Sciences, University of EmbuInstitute for Biotechnology Research, Jomo Kenyatta University of Agriculture and TechnologyDepartment of Life Sciences, South Eastern Kenya UniversityDepartment of Medical Microbiology, Jomo Kenyatta University of Agriculture and TechnologyDepartment of Biological Sciences, University of EmbuAbstract Background Diversification of global food systems through exploration of traditional varieties and wild edible plant species is a focal mitigation strategy for food security worldwide. The present study determined the phenomic diversity of locally available, affordable and climate-resilient cultivated and wild Crotalaria species for breeding purposes. Methods Seed samples were collected from different administrative counties in Kenya spanning different climatic zones. Other seeds were provided by the Genetic Resources Research Institute of Kenya. A randomized complete block design with three replications was used for agro-morphological evaluation of the 83 accessions used in this study. Data on quantitative and qualitative traits was collected. Cluster analysis on R and R-studio was used to generate a dendrogram by the Euclidian genetic distance and dissimilarity indices while the non-metric multidimensional scaling (NMDS) method was used to determine the spatial interrelationship between the accessions. The Pearson’s correlation coefficients were used to determine the relationships between qualitative and quantitative traits while the principal component analysis was used to discriminate the accessions. Results Three edible species (C. brevidens Benth., C. ochroleuca G.Don, C. trichotoma Bojer.) were found to be cultivated by Kenyan farmers and a significant variation (p < 0.0001) for all parameters under study was recorded. Agglomerative hierarchical clustering grouped the accessions into 8 major clusters. The NMDS ordination formed 15 and 6 groups based on counties and regions respectively. Conclusions This study forms the basis for exploring novel breeding strategies among cultivated species, between wild and cultivated Crotalaria species as well as determining molecular markers linked to phenomic traits.https://doi.org/10.1186/s43170-021-00031-0CrotalariaGermplasmAgro-morphologyGenetic diversity |
| spellingShingle | Joshua Kiilu Muli Johnstone O. Neondo Peter K. Kamau Eddy Odari Nancy L. M. Budambula Phenomic characterization of Crotalaria germplasm for crop improvement CABI Agriculture and Bioscience Crotalaria Germplasm Agro-morphology Genetic diversity |
| title | Phenomic characterization of Crotalaria germplasm for crop improvement |
| title_full | Phenomic characterization of Crotalaria germplasm for crop improvement |
| title_fullStr | Phenomic characterization of Crotalaria germplasm for crop improvement |
| title_full_unstemmed | Phenomic characterization of Crotalaria germplasm for crop improvement |
| title_short | Phenomic characterization of Crotalaria germplasm for crop improvement |
| title_sort | phenomic characterization of crotalaria germplasm for crop improvement |
| topic | Crotalaria Germplasm Agro-morphology Genetic diversity |
| url | https://doi.org/10.1186/s43170-021-00031-0 |
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