Sequences of acetyl CoA carboxylase promoter for tumour necrosis factor action
Tumour necrosis factor (TNF) inhibits the accumulation of acetyl CoA carboxylase (ACC) mRNA by decreasing the rate of ACC gene transcription. The ACC mRNA species found in 30A5 cells are generated from promoter II and TNF inhibits the accumulation of class 2 type mRNAs. By using 5' deletion mut...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1993-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935193000377 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564337368104960 |
|---|---|
| author | Keerang Park Michael E. Pape Ki-Han Kim |
| author_facet | Keerang Park Michael E. Pape Ki-Han Kim |
| author_sort | Keerang Park |
| collection | DOAJ |
| description | Tumour necrosis factor (TNF) inhibits the accumulation of acetyl CoA carboxylase (ACC) mRNA by decreasing the rate of ACC gene transcription. The ACC mRNA species found in 30A5 cells are generated from promoter II and TNF inhibits the accumulation of class 2 type mRNAs. By using 5' deletion mutants of promoter II fused to the bacterial chloramphenicol acetyltransferase (CAT) gene, the DNA mobility shift assay and the DNase I footprinting assay, the authors have identified the 30 bp from −389 to −359 as the TNF responsive element in promoter II. TNF treatment causes a decrease in the binding activity of nuclear protein(s) specific to the TNF responsive element. When the fragment containing the TNF responsive element was incorporated into the thymidine kinase promoter, the chimeric gene exhibited TNF induced inhibition of expression. |
| format | Article |
| id | doaj-art-70d4dd0aea514ab8a6f2170db8e5b3c5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1993-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-70d4dd0aea514ab8a6f2170db8e5b3c52025-02-03T01:11:20ZengWileyMediators of Inflammation0962-93511466-18611993-01-012427127710.1155/S0962935193000377Sequences of acetyl CoA carboxylase promoter for tumour necrosis factor actionKeerang Park0Michael E. Pape1Ki-Han Kim2Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USADepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USADepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USATumour necrosis factor (TNF) inhibits the accumulation of acetyl CoA carboxylase (ACC) mRNA by decreasing the rate of ACC gene transcription. The ACC mRNA species found in 30A5 cells are generated from promoter II and TNF inhibits the accumulation of class 2 type mRNAs. By using 5' deletion mutants of promoter II fused to the bacterial chloramphenicol acetyltransferase (CAT) gene, the DNA mobility shift assay and the DNase I footprinting assay, the authors have identified the 30 bp from −389 to −359 as the TNF responsive element in promoter II. TNF treatment causes a decrease in the binding activity of nuclear protein(s) specific to the TNF responsive element. When the fragment containing the TNF responsive element was incorporated into the thymidine kinase promoter, the chimeric gene exhibited TNF induced inhibition of expression.http://dx.doi.org/10.1155/S0962935193000377 |
| spellingShingle | Keerang Park Michael E. Pape Ki-Han Kim Sequences of acetyl CoA carboxylase promoter for tumour necrosis factor action Mediators of Inflammation |
| title | Sequences of acetyl CoA carboxylase promoter for tumour necrosis factor action |
| title_full | Sequences of acetyl CoA carboxylase promoter for tumour necrosis factor action |
| title_fullStr | Sequences of acetyl CoA carboxylase promoter for tumour necrosis factor action |
| title_full_unstemmed | Sequences of acetyl CoA carboxylase promoter for tumour necrosis factor action |
| title_short | Sequences of acetyl CoA carboxylase promoter for tumour necrosis factor action |
| title_sort | sequences of acetyl coa carboxylase promoter for tumour necrosis factor action |
| url | http://dx.doi.org/10.1155/S0962935193000377 |
| work_keys_str_mv | AT keerangpark sequencesofacetylcoacarboxylasepromoterfortumournecrosisfactoraction AT michaelepape sequencesofacetylcoacarboxylasepromoterfortumournecrosisfactoraction AT kihankim sequencesofacetylcoacarboxylasepromoterfortumournecrosisfactoraction |