TLR7 Polymorphism (rs179008 and rs179009) in HIV-Infected Individual Naïve to ART
Toll-like receptors (TLRs) play an important role in the innate immune response to HIV infection. Single nucleotide polymorphism (SNP) in TLR7 (Gln11Leu) gene has been associated with a rapid decline of CD4T cell count. Hence, we assessed the TLR7 (rs179008, Gln11Leu (A/T) and rs179009, IVS2-151 (A/...
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/6702169 |
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| author | HariOm Singh Dharmesh Samani Sumit Aggarwal |
| author_facet | HariOm Singh Dharmesh Samani Sumit Aggarwal |
| author_sort | HariOm Singh |
| collection | DOAJ |
| description | Toll-like receptors (TLRs) play an important role in the innate immune response to HIV infection. Single nucleotide polymorphism (SNP) in TLR7 (Gln11Leu) gene has been associated with a rapid decline of CD4T cell count. Hence, we assessed the TLR7 (rs179008, Gln11Leu (A/T) and rs179009, IVS2-151 (A/G)) polymorphism in 150 HIV-infected individuals naïve to ART and 158 healthy controls. The genotyping of TLR7 Gln11Leu (A/T) and IVS2-151 (A/G) polymorphisms was done using the PCR-RFLP method. In univariate analysis, none of the genotype and haplotype of TLR7 Gln11Leu (A/T) and IVS2-151 (A/G) polymorphism differed significantly between HIV-infected individuals and healthy controls. The occurrence of TLR7 rs179009AG genotype in the codominant model and rs179009 AG-GG genotype in the dominant model was significantly reduced in HIV-infected individuals as compared to healthy controls (18.0% vs. 29.1%, OR=0.42, P=0.016; 26.7% vs. 36.7%, OR=0.52, P=0.016). TLR7 rs179009AG genotype was significantly underrepresented in the intermediate HIV disease stage compared with healthy controls (OR=0.03, P=0.04). TLR7 rs179009AG genotype expressed higher in tobacco-consuming HIV-infected individuals compared with nonusers (OR=1.71, P=0.47). In conclusion, rs179009 AG-GG and AG genotypes were found reduced in HIV-infected individuals as compared to healthy controls; their higher prevalence in health individuals clearly support that they are associated with reduced risk of acquisition of HIV-1 infection. |
| format | Article |
| id | doaj-art-70aa1842414649f1a9507121ee981cc4 |
| institution | Kabale University |
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| language | English |
| publishDate | 2020-01-01 |
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| series | Mediators of Inflammation |
| spelling | doaj-art-70aa1842414649f1a9507121ee981cc42025-02-03T06:46:08ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/67021696702169TLR7 Polymorphism (rs179008 and rs179009) in HIV-Infected Individual Naïve to ARTHariOm Singh0Dharmesh Samani1Sumit Aggarwal2Department of Molecular Biology, National AIDS Research Institute Pune, 411026, IndiaDepartment of Molecular Biology, National AIDS Research Institute Pune, 411026, IndiaDivision of ECD, Indian Council of Medical Research-HQ, New Delhi 110029, IndiaToll-like receptors (TLRs) play an important role in the innate immune response to HIV infection. Single nucleotide polymorphism (SNP) in TLR7 (Gln11Leu) gene has been associated with a rapid decline of CD4T cell count. Hence, we assessed the TLR7 (rs179008, Gln11Leu (A/T) and rs179009, IVS2-151 (A/G)) polymorphism in 150 HIV-infected individuals naïve to ART and 158 healthy controls. The genotyping of TLR7 Gln11Leu (A/T) and IVS2-151 (A/G) polymorphisms was done using the PCR-RFLP method. In univariate analysis, none of the genotype and haplotype of TLR7 Gln11Leu (A/T) and IVS2-151 (A/G) polymorphism differed significantly between HIV-infected individuals and healthy controls. The occurrence of TLR7 rs179009AG genotype in the codominant model and rs179009 AG-GG genotype in the dominant model was significantly reduced in HIV-infected individuals as compared to healthy controls (18.0% vs. 29.1%, OR=0.42, P=0.016; 26.7% vs. 36.7%, OR=0.52, P=0.016). TLR7 rs179009AG genotype was significantly underrepresented in the intermediate HIV disease stage compared with healthy controls (OR=0.03, P=0.04). TLR7 rs179009AG genotype expressed higher in tobacco-consuming HIV-infected individuals compared with nonusers (OR=1.71, P=0.47). In conclusion, rs179009 AG-GG and AG genotypes were found reduced in HIV-infected individuals as compared to healthy controls; their higher prevalence in health individuals clearly support that they are associated with reduced risk of acquisition of HIV-1 infection.http://dx.doi.org/10.1155/2020/6702169 |
| spellingShingle | HariOm Singh Dharmesh Samani Sumit Aggarwal TLR7 Polymorphism (rs179008 and rs179009) in HIV-Infected Individual Naïve to ART Mediators of Inflammation |
| title | TLR7 Polymorphism (rs179008 and rs179009) in HIV-Infected Individual Naïve to ART |
| title_full | TLR7 Polymorphism (rs179008 and rs179009) in HIV-Infected Individual Naïve to ART |
| title_fullStr | TLR7 Polymorphism (rs179008 and rs179009) in HIV-Infected Individual Naïve to ART |
| title_full_unstemmed | TLR7 Polymorphism (rs179008 and rs179009) in HIV-Infected Individual Naïve to ART |
| title_short | TLR7 Polymorphism (rs179008 and rs179009) in HIV-Infected Individual Naïve to ART |
| title_sort | tlr7 polymorphism rs179008 and rs179009 in hiv infected individual naive to art |
| url | http://dx.doi.org/10.1155/2020/6702169 |
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