Data Mining Evidences Variabilities in Glucose and Lipid Metabolism among Fish Strains: A Case Study on Three Genotypes of Gibel Carp Fed by Different Carbohydrate Sources
An 8-week feeding trial was conducted to evaluate the application of common carbohydrate sources, cornstarch (CS), wheat starch (WS), and wheat flour (WF), to different gibel carp genotypes, Dongting, CASIII, and CASV. The results of the growth and physical responses were analysed by data visualizat...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
|
| Series: | Aquaculture Nutrition |
| Online Access: | http://dx.doi.org/10.1155/2023/7589827 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832559490504851456 |
|---|---|
| author | Xuerong Song Haokun Liu Junyan Jin Dong Han Xiaoming Zhu Yunxia Yang Shouqi Xie |
| author_facet | Xuerong Song Haokun Liu Junyan Jin Dong Han Xiaoming Zhu Yunxia Yang Shouqi Xie |
| author_sort | Xuerong Song |
| collection | DOAJ |
| description | An 8-week feeding trial was conducted to evaluate the application of common carbohydrate sources, cornstarch (CS), wheat starch (WS), and wheat flour (WF), to different gibel carp genotypes, Dongting, CASIII, and CASV. The results of the growth and physical responses were analysed by data visualization and unsupervised machine learning. As revealed by a self-organizing map (SOM) and the cluster of growth and biochemical indicators, CASV had superior growth and feed utilization and better regulation of postprandial glucose, followed by CASIII, while Dongting showed a high level of plasma glucose with poor growth performance. CS, WS, and WF were differently utilized by the gibel carp, and WF was associated with greater zootechnical performance based on higher specific growth rate (SGR), feed efficiency (FE), protein retention efficiency (PRE), and lipid retention efficiency (LRE), induced hepatic lipogenesis, increased liver lipids, and enhanced muscle glycogen. Spearman’s correlation analysis of the physiological responses indicated that plasma glucose had a significantly negative correlation with growth, feed utilization, glycogen storage, and plasma cholesterol level, and it was positively related to liver fat content in gibel carp. Transcriptional variabilities were observed: CASIII showed increased expression of pklr, which is involved in hepatic glycolysis, and pck and g6p, which are involved in gluconeogenesis. Interestingly, Dongting showed upregulation of genes involved in glycolysis and fatty acid oxidation in muscle. Furthermore, there were numerous interactions between carbohydrate sources and strains for growth, metabolites, and transcriptional control, confirming the existence of genetic polymorphisms in carbohydrate use in gibel carp. Globally, CASV showed relatively better growth and carbohydrate utilization, and wheat flour seemed to be more efficiently utilized by gibel carp. |
| format | Article |
| id | doaj-art-7099368bf3ac442595f6bf84fcc614a7 |
| institution | Kabale University |
| issn | 1365-2095 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture Nutrition |
| spelling | doaj-art-7099368bf3ac442595f6bf84fcc614a72025-02-03T01:29:51ZengWileyAquaculture Nutrition1365-20952023-01-01202310.1155/2023/7589827Data Mining Evidences Variabilities in Glucose and Lipid Metabolism among Fish Strains: A Case Study on Three Genotypes of Gibel Carp Fed by Different Carbohydrate SourcesXuerong Song0Haokun Liu1Junyan Jin2Dong Han3Xiaoming Zhu4Yunxia Yang5Shouqi Xie6State Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyAn 8-week feeding trial was conducted to evaluate the application of common carbohydrate sources, cornstarch (CS), wheat starch (WS), and wheat flour (WF), to different gibel carp genotypes, Dongting, CASIII, and CASV. The results of the growth and physical responses were analysed by data visualization and unsupervised machine learning. As revealed by a self-organizing map (SOM) and the cluster of growth and biochemical indicators, CASV had superior growth and feed utilization and better regulation of postprandial glucose, followed by CASIII, while Dongting showed a high level of plasma glucose with poor growth performance. CS, WS, and WF were differently utilized by the gibel carp, and WF was associated with greater zootechnical performance based on higher specific growth rate (SGR), feed efficiency (FE), protein retention efficiency (PRE), and lipid retention efficiency (LRE), induced hepatic lipogenesis, increased liver lipids, and enhanced muscle glycogen. Spearman’s correlation analysis of the physiological responses indicated that plasma glucose had a significantly negative correlation with growth, feed utilization, glycogen storage, and plasma cholesterol level, and it was positively related to liver fat content in gibel carp. Transcriptional variabilities were observed: CASIII showed increased expression of pklr, which is involved in hepatic glycolysis, and pck and g6p, which are involved in gluconeogenesis. Interestingly, Dongting showed upregulation of genes involved in glycolysis and fatty acid oxidation in muscle. Furthermore, there were numerous interactions between carbohydrate sources and strains for growth, metabolites, and transcriptional control, confirming the existence of genetic polymorphisms in carbohydrate use in gibel carp. Globally, CASV showed relatively better growth and carbohydrate utilization, and wheat flour seemed to be more efficiently utilized by gibel carp.http://dx.doi.org/10.1155/2023/7589827 |
| spellingShingle | Xuerong Song Haokun Liu Junyan Jin Dong Han Xiaoming Zhu Yunxia Yang Shouqi Xie Data Mining Evidences Variabilities in Glucose and Lipid Metabolism among Fish Strains: A Case Study on Three Genotypes of Gibel Carp Fed by Different Carbohydrate Sources Aquaculture Nutrition |
| title | Data Mining Evidences Variabilities in Glucose and Lipid Metabolism among Fish Strains: A Case Study on Three Genotypes of Gibel Carp Fed by Different Carbohydrate Sources |
| title_full | Data Mining Evidences Variabilities in Glucose and Lipid Metabolism among Fish Strains: A Case Study on Three Genotypes of Gibel Carp Fed by Different Carbohydrate Sources |
| title_fullStr | Data Mining Evidences Variabilities in Glucose and Lipid Metabolism among Fish Strains: A Case Study on Three Genotypes of Gibel Carp Fed by Different Carbohydrate Sources |
| title_full_unstemmed | Data Mining Evidences Variabilities in Glucose and Lipid Metabolism among Fish Strains: A Case Study on Three Genotypes of Gibel Carp Fed by Different Carbohydrate Sources |
| title_short | Data Mining Evidences Variabilities in Glucose and Lipid Metabolism among Fish Strains: A Case Study on Three Genotypes of Gibel Carp Fed by Different Carbohydrate Sources |
| title_sort | data mining evidences variabilities in glucose and lipid metabolism among fish strains a case study on three genotypes of gibel carp fed by different carbohydrate sources |
| url | http://dx.doi.org/10.1155/2023/7589827 |
| work_keys_str_mv | AT xuerongsong dataminingevidencesvariabilitiesinglucoseandlipidmetabolismamongfishstrainsacasestudyonthreegenotypesofgibelcarpfedbydifferentcarbohydratesources AT haokunliu dataminingevidencesvariabilitiesinglucoseandlipidmetabolismamongfishstrainsacasestudyonthreegenotypesofgibelcarpfedbydifferentcarbohydratesources AT junyanjin dataminingevidencesvariabilitiesinglucoseandlipidmetabolismamongfishstrainsacasestudyonthreegenotypesofgibelcarpfedbydifferentcarbohydratesources AT donghan dataminingevidencesvariabilitiesinglucoseandlipidmetabolismamongfishstrainsacasestudyonthreegenotypesofgibelcarpfedbydifferentcarbohydratesources AT xiaomingzhu dataminingevidencesvariabilitiesinglucoseandlipidmetabolismamongfishstrainsacasestudyonthreegenotypesofgibelcarpfedbydifferentcarbohydratesources AT yunxiayang dataminingevidencesvariabilitiesinglucoseandlipidmetabolismamongfishstrainsacasestudyonthreegenotypesofgibelcarpfedbydifferentcarbohydratesources AT shouqixie dataminingevidencesvariabilitiesinglucoseandlipidmetabolismamongfishstrainsacasestudyonthreegenotypesofgibelcarpfedbydifferentcarbohydratesources |