<i>Vismia guianensis</i> Improves Survival of <i>Tenebrio molitor</i> and Mice During Lethal Infection with <i>Candida albicans</i>
<b>Background/Objectives</b>: <i>Vismia guianensis</i> is a vegetal species popularly used to treat fungal infections. This study evaluated the anti-<i>Candida</i> effect of <i>V. guianensis</i> extract after <i>C. albicans</i> lethal infec...
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2025-01-01
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| author | Arthur André Castro Costa Elizangela Pestana Motta Aluísio Silva Oliveira Pamela Gomes Santos Josivan Regis Farias Danielle Cristine Gomes Franco Mayara Cristina Pinto Silva Nicolle Teixeira Barbosa Simone Batista Muniz Luís Douglas Miranda. Silva Lucilene Amorim Silva Claudia Quintino Rocha Flavia Raquel Fernandes Nascimento Rosane Nassar Meireles Guerra |
| author_facet | Arthur André Castro Costa Elizangela Pestana Motta Aluísio Silva Oliveira Pamela Gomes Santos Josivan Regis Farias Danielle Cristine Gomes Franco Mayara Cristina Pinto Silva Nicolle Teixeira Barbosa Simone Batista Muniz Luís Douglas Miranda. Silva Lucilene Amorim Silva Claudia Quintino Rocha Flavia Raquel Fernandes Nascimento Rosane Nassar Meireles Guerra |
| author_sort | Arthur André Castro Costa |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: <i>Vismia guianensis</i> is a vegetal species popularly used to treat fungal infections. This study evaluated the anti-<i>Candida</i> effect of <i>V. guianensis</i> extract after <i>C. albicans</i> lethal infection in <i>Tenebrio molitor</i> larvae and mice. <b>Methods and Results</b>: The chemical profile analysis of a hydroethanolic extract of the leaves of <i>V. guianensis</i> (EHVG) identified 14 compounds. Two sets of experiments used <i>T. molitor</i> larvae. To evaluate toxicity, the uninfected larvae were treated with EHVG or anthraquinone. We considered the following groups: the controls received PBS; ANFO B received amphotericin B (600 mg/mL); EHVG received the extract; and ANTQ received anthraquinone. The extract and anthraquinone resulted in low-level toxicity in the <i>T. molitor</i> larvae. Another set of experiments evaluated the EHVG effect during lethal infection with <i>Candida albicans</i>. The <i>T. molitor</i> larvae were treated intracelomically (ic/10 μL). Treatment with EHVG efficiently improved the survival of the larvae after lethal infection (60%), probably due to the reduction in CFUs. In the mice, the antifungal effect of EHVG was determined in three groups of immunosuppressed Swiss mice (cyclophosphamide, 50 mg/kg/ip) infected with <i>C. albicans</i> (1 × 10<sup>7</sup> CFU/ip). The control animals were infected and untreated; the ANFO B animals were infected and treated with amphotericin B (600 µg/kg/ip); and the EHVG animals were infected and treated with the extract (5 mg/kg/orally). A SHAM group (uninfected and untreated) was also included. Survival was assessed for 5 days. The extract increased the mice’s survival (60%) and life expectancy, reducing the CFU counts in the peritoneum and blood. EHVG also increased the number of blood neutrophils and peritoneal macrophages. These systemic activities are likely associated with the presence of flavonoids in the extract. <b>Conclusions</b>: The beneficial effects of EHVG in lethal sepsis are related to an antifungal effect, with the number of CFUs decreasing in the larvae and the mice. In addition, EHVG showed immunological activity in the mice, considering immune cell distribution and cytokine production. |
| format | Article |
| id | doaj-art-7083ce3df02a4847a4adf0704045d254 |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Antibiotics |
| spelling | doaj-art-7083ce3df02a4847a4adf0704045d2542025-01-24T13:18:51ZengMDPI AGAntibiotics2079-63822025-01-011417210.3390/antibiotics14010072<i>Vismia guianensis</i> Improves Survival of <i>Tenebrio molitor</i> and Mice During Lethal Infection with <i>Candida albicans</i>Arthur André Castro Costa0Elizangela Pestana Motta1Aluísio Silva Oliveira2Pamela Gomes Santos3Josivan Regis Farias4Danielle Cristine Gomes Franco5Mayara Cristina Pinto Silva6Nicolle Teixeira Barbosa7Simone Batista Muniz8Luís Douglas Miranda. Silva9Lucilene Amorim Silva10Claudia Quintino Rocha11Flavia Raquel Fernandes Nascimento12Rosane Nassar Meireles Guerra13Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Ensino Integrado, Bloco 3, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Ensino Integrado, Bloco 3, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Ensino Integrado, Bloco 3, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Ensino Integrado, Bloco 3, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Ensino Integrado, Bloco 3, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Ensino Integrado, Bloco 3, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilLaboratory of Immunophysiology, Universidade Federal do Maranhão, Ensino Integrado, Bloco 1, Sala 1A, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Ensino Integrado, Bloco 3, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Ensino Integrado, Bloco 3, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Ensino Integrado, Bloco 3, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Ensino Integrado, Bloco 3, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilLaboratory of Chemistry of Natural Products, Centro de Ciências Exatas e Tecnológicas, Universidade Federal do Maranhão, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Ensino Integrado, Bloco 3, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Ensino Integrado, Bloco 3, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, Brazil<b>Background/Objectives</b>: <i>Vismia guianensis</i> is a vegetal species popularly used to treat fungal infections. This study evaluated the anti-<i>Candida</i> effect of <i>V. guianensis</i> extract after <i>C. albicans</i> lethal infection in <i>Tenebrio molitor</i> larvae and mice. <b>Methods and Results</b>: The chemical profile analysis of a hydroethanolic extract of the leaves of <i>V. guianensis</i> (EHVG) identified 14 compounds. Two sets of experiments used <i>T. molitor</i> larvae. To evaluate toxicity, the uninfected larvae were treated with EHVG or anthraquinone. We considered the following groups: the controls received PBS; ANFO B received amphotericin B (600 mg/mL); EHVG received the extract; and ANTQ received anthraquinone. The extract and anthraquinone resulted in low-level toxicity in the <i>T. molitor</i> larvae. Another set of experiments evaluated the EHVG effect during lethal infection with <i>Candida albicans</i>. The <i>T. molitor</i> larvae were treated intracelomically (ic/10 μL). Treatment with EHVG efficiently improved the survival of the larvae after lethal infection (60%), probably due to the reduction in CFUs. In the mice, the antifungal effect of EHVG was determined in three groups of immunosuppressed Swiss mice (cyclophosphamide, 50 mg/kg/ip) infected with <i>C. albicans</i> (1 × 10<sup>7</sup> CFU/ip). The control animals were infected and untreated; the ANFO B animals were infected and treated with amphotericin B (600 µg/kg/ip); and the EHVG animals were infected and treated with the extract (5 mg/kg/orally). A SHAM group (uninfected and untreated) was also included. Survival was assessed for 5 days. The extract increased the mice’s survival (60%) and life expectancy, reducing the CFU counts in the peritoneum and blood. EHVG also increased the number of blood neutrophils and peritoneal macrophages. These systemic activities are likely associated with the presence of flavonoids in the extract. <b>Conclusions</b>: The beneficial effects of EHVG in lethal sepsis are related to an antifungal effect, with the number of CFUs decreasing in the larvae and the mice. In addition, EHVG showed immunological activity in the mice, considering immune cell distribution and cytokine production.https://www.mdpi.com/2079-6382/14/1/72antifungalmacrophageneutrophilsepsisTNFα<i>Tenebrio molitor</i> |
| spellingShingle | Arthur André Castro Costa Elizangela Pestana Motta Aluísio Silva Oliveira Pamela Gomes Santos Josivan Regis Farias Danielle Cristine Gomes Franco Mayara Cristina Pinto Silva Nicolle Teixeira Barbosa Simone Batista Muniz Luís Douglas Miranda. Silva Lucilene Amorim Silva Claudia Quintino Rocha Flavia Raquel Fernandes Nascimento Rosane Nassar Meireles Guerra <i>Vismia guianensis</i> Improves Survival of <i>Tenebrio molitor</i> and Mice During Lethal Infection with <i>Candida albicans</i> Antibiotics antifungal macrophage neutrophil sepsis TNFα <i>Tenebrio molitor</i> |
| title | <i>Vismia guianensis</i> Improves Survival of <i>Tenebrio molitor</i> and Mice During Lethal Infection with <i>Candida albicans</i> |
| title_full | <i>Vismia guianensis</i> Improves Survival of <i>Tenebrio molitor</i> and Mice During Lethal Infection with <i>Candida albicans</i> |
| title_fullStr | <i>Vismia guianensis</i> Improves Survival of <i>Tenebrio molitor</i> and Mice During Lethal Infection with <i>Candida albicans</i> |
| title_full_unstemmed | <i>Vismia guianensis</i> Improves Survival of <i>Tenebrio molitor</i> and Mice During Lethal Infection with <i>Candida albicans</i> |
| title_short | <i>Vismia guianensis</i> Improves Survival of <i>Tenebrio molitor</i> and Mice During Lethal Infection with <i>Candida albicans</i> |
| title_sort | i vismia guianensis i improves survival of i tenebrio molitor i and mice during lethal infection with i candida albicans i |
| topic | antifungal macrophage neutrophil sepsis TNFα <i>Tenebrio molitor</i> |
| url | https://www.mdpi.com/2079-6382/14/1/72 |
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