Characterizing Antimicrobial Effects of Radiation and Antibiotic Interactions on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> Using MALDI-TOF MS
<b>Background/Objectives:</b> Antibiotic-resistant bacteria are becoming a major challenge in human and veterinary medicine, as well as in food processing. <b>Methods:</b> In this study, the protein diversity in antibiotic-sensitive and -resistant strains of <i>Staphylo...
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2025-01-01
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| author | Ali Haider Renáta Homlok Csilla Mohácsiné Farkas Tamás Kocsis |
| author_facet | Ali Haider Renáta Homlok Csilla Mohácsiné Farkas Tamás Kocsis |
| author_sort | Ali Haider |
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| description | <b>Background/Objectives:</b> Antibiotic-resistant bacteria are becoming a major challenge in human and veterinary medicine, as well as in food processing. <b>Methods:</b> In this study, the protein diversity in antibiotic-sensitive and -resistant strains of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> was investigated by exposing them to varying doses of gamma irradiation, with and without antibiotic presence. Changes in bacterial protein profiles were characterized using MALDI-TOF MS to reveal dose-dependent adaptations and potentiation effects under combined irradiation and antibiotic treatments. <b>Results:</b> The results indicate that MALDI-TOF MS effectively differentiates between sensitive and resistant strains, particularly at lower radiation doses (0, 0.2, and 0.4 kGy), with distinct separation in protein spectra. However, at 0.6 kGy, protein profiles plateaued, suggesting a potential threshold effect in radiation response. In 24-h cultures from irradiated <i>Staphylococcus aureus</i>, significant differences emerged in the resistant strain at 0.6 kGy in the presence of antibiotics, with further generational divergence dependent on initial antibiotic exposure. In the case of the sensitive strain, profiles were notably distinct at the 0.4 and 0.6 kGy doses, revealing dose- and treatment-specific responses. For <i>Escherichia coli</i>, generational differences between resistant and sensitive strains were apparent, though antibiotic effects on protein profiles were limited to the 0.6 kGy dose. <b>Conclusions:</b> The results underscore a potentiation interaction between irradiation and antibiotic exposure, affecting protein diversity and adaptation. Sensitive strains displayed heightened proteomic responses to minor treatment variations, while resistant strains exhibited more stable profiles across conditions. The findings highlight MALDI-TOF MS as a valuable tool in detecting proteomic biomarkers linked to bacterial resistance and stress adaptation. |
| format | Article |
| id | doaj-art-70514af0ddad409aa1ff36b83c1f48fc |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj-art-70514af0ddad409aa1ff36b83c1f48fc2025-01-24T13:18:41ZengMDPI AGAntibiotics2079-63822025-01-011414110.3390/antibiotics14010041Characterizing Antimicrobial Effects of Radiation and Antibiotic Interactions on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> Using MALDI-TOF MSAli Haider0Renáta Homlok1Csilla Mohácsiné Farkas2Tamás Kocsis3Doctoral School of Food Sciences, Hungarian University of Agriculture and Life Sciences, H-1118 Budapest, HungaryHUN-REN Centre for Energy Research, H-1121 Budapest, HungaryDepartment of Food Microbiology, Hygiene, and Safety, Hungarian University of Agriculture and Life Sciences, H-1118 Budapest, HungaryDepartment of Food Microbiology, Hygiene, and Safety, Hungarian University of Agriculture and Life Sciences, H-1118 Budapest, Hungary<b>Background/Objectives:</b> Antibiotic-resistant bacteria are becoming a major challenge in human and veterinary medicine, as well as in food processing. <b>Methods:</b> In this study, the protein diversity in antibiotic-sensitive and -resistant strains of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> was investigated by exposing them to varying doses of gamma irradiation, with and without antibiotic presence. Changes in bacterial protein profiles were characterized using MALDI-TOF MS to reveal dose-dependent adaptations and potentiation effects under combined irradiation and antibiotic treatments. <b>Results:</b> The results indicate that MALDI-TOF MS effectively differentiates between sensitive and resistant strains, particularly at lower radiation doses (0, 0.2, and 0.4 kGy), with distinct separation in protein spectra. However, at 0.6 kGy, protein profiles plateaued, suggesting a potential threshold effect in radiation response. In 24-h cultures from irradiated <i>Staphylococcus aureus</i>, significant differences emerged in the resistant strain at 0.6 kGy in the presence of antibiotics, with further generational divergence dependent on initial antibiotic exposure. In the case of the sensitive strain, profiles were notably distinct at the 0.4 and 0.6 kGy doses, revealing dose- and treatment-specific responses. For <i>Escherichia coli</i>, generational differences between resistant and sensitive strains were apparent, though antibiotic effects on protein profiles were limited to the 0.6 kGy dose. <b>Conclusions:</b> The results underscore a potentiation interaction between irradiation and antibiotic exposure, affecting protein diversity and adaptation. Sensitive strains displayed heightened proteomic responses to minor treatment variations, while resistant strains exhibited more stable profiles across conditions. The findings highlight MALDI-TOF MS as a valuable tool in detecting proteomic biomarkers linked to bacterial resistance and stress adaptation.https://www.mdpi.com/2079-6382/14/1/41<i>Escherichia coli</i>gamma irradiationMALDI-TOFpiperacillinprotein<i>Staphylococcus aureus</i> |
| spellingShingle | Ali Haider Renáta Homlok Csilla Mohácsiné Farkas Tamás Kocsis Characterizing Antimicrobial Effects of Radiation and Antibiotic Interactions on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> Using MALDI-TOF MS Antibiotics <i>Escherichia coli</i> gamma irradiation MALDI-TOF piperacillin protein <i>Staphylococcus aureus</i> |
| title | Characterizing Antimicrobial Effects of Radiation and Antibiotic Interactions on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> Using MALDI-TOF MS |
| title_full | Characterizing Antimicrobial Effects of Radiation and Antibiotic Interactions on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> Using MALDI-TOF MS |
| title_fullStr | Characterizing Antimicrobial Effects of Radiation and Antibiotic Interactions on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> Using MALDI-TOF MS |
| title_full_unstemmed | Characterizing Antimicrobial Effects of Radiation and Antibiotic Interactions on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> Using MALDI-TOF MS |
| title_short | Characterizing Antimicrobial Effects of Radiation and Antibiotic Interactions on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> Using MALDI-TOF MS |
| title_sort | characterizing antimicrobial effects of radiation and antibiotic interactions on i staphylococcus aureus i and i escherichia coli i using maldi tof ms |
| topic | <i>Escherichia coli</i> gamma irradiation MALDI-TOF piperacillin protein <i>Staphylococcus aureus</i> |
| url | https://www.mdpi.com/2079-6382/14/1/41 |
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