Enhanced Healing of Diabetic Wounds by Subcutaneous Administration of Human Umbilical Cord Derived Stem Cells and Their Conditioned Media
Objective. Mesenchymal stem cells (MSCs) isolated from the umbilical cord and their conditioned media (CM) can be easily obtained and refined compared with stem cells from other sources. Here, we explore the possibility of the benefits of these cells in healing diabetic wounds. Methodology and Resul...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2013/592454 |
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| author | Chandrama Shrestha Liling Zhao Ke Chen Honghui He Zhaohui Mo |
| author_facet | Chandrama Shrestha Liling Zhao Ke Chen Honghui He Zhaohui Mo |
| author_sort | Chandrama Shrestha |
| collection | DOAJ |
| description | Objective. Mesenchymal stem cells (MSCs) isolated from the umbilical cord and their conditioned media (CM) can be easily obtained and refined compared with stem cells from other sources. Here, we explore the possibility of the benefits of these cells in healing diabetic wounds. Methodology and Results. Delayed wound healing animal models were established by making a standard wound on the dorsum of eighteen db/db mice, which were divided into three groups with six mice in each: groups I, II, and III received PBS, UC-MSC, and CM, respectively. UC-MSC and their CM significantly accelerated wound closure compared to PBS-treated wounds, and it was most rapid in CM-injected wounds. In day-14 wounds, significant difference in capillary densities among the three groups was noted (n=6; P<0.05), and higher levels of VEGF, PDGF, and KGF expression in the CM- and UC-MSC-injected wounds compared to the PBS-treated wounds were seen. The expression levels of PDGF-β and KGF were higher in CM-treated wounds than those in UC-MSC-treated wounds. Conclusion. Both the transplantation of UC-MSC and their CM are beneficial to diabetic wound healing, and CM has been shown to be therapeutically better than UC-MSC, at least in the context of diabetic wound healing. |
| format | Article |
| id | doaj-art-70477005151a444d96692ac255ee4594 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-70477005151a444d96692ac255ee45942025-02-03T01:12:08ZengWileyInternational Journal of Endocrinology1687-83371687-83452013-01-01201310.1155/2013/592454592454Enhanced Healing of Diabetic Wounds by Subcutaneous Administration of Human Umbilical Cord Derived Stem Cells and Their Conditioned MediaChandrama Shrestha0Liling Zhao1Ke Chen2Honghui He3Zhaohui Mo4Department of Endocrinology and Metabolism, The Third Xiangya Hospital, Central South University, Changsha 410013, ChinaDepartment of Endocrinology and Metabolism, The Third Xiangya Hospital, Central South University, Changsha 410013, ChinaDepartment of Endocrinology and Metabolism, The Third Xiangya Hospital, Central South University, Changsha 410013, ChinaDepartment of Endocrinology and Metabolism, The Third Xiangya Hospital, Central South University, Changsha 410013, ChinaDepartment of Endocrinology and Metabolism, The Third Xiangya Hospital, Central South University, Changsha 410013, ChinaObjective. Mesenchymal stem cells (MSCs) isolated from the umbilical cord and their conditioned media (CM) can be easily obtained and refined compared with stem cells from other sources. Here, we explore the possibility of the benefits of these cells in healing diabetic wounds. Methodology and Results. Delayed wound healing animal models were established by making a standard wound on the dorsum of eighteen db/db mice, which were divided into three groups with six mice in each: groups I, II, and III received PBS, UC-MSC, and CM, respectively. UC-MSC and their CM significantly accelerated wound closure compared to PBS-treated wounds, and it was most rapid in CM-injected wounds. In day-14 wounds, significant difference in capillary densities among the three groups was noted (n=6; P<0.05), and higher levels of VEGF, PDGF, and KGF expression in the CM- and UC-MSC-injected wounds compared to the PBS-treated wounds were seen. The expression levels of PDGF-β and KGF were higher in CM-treated wounds than those in UC-MSC-treated wounds. Conclusion. Both the transplantation of UC-MSC and their CM are beneficial to diabetic wound healing, and CM has been shown to be therapeutically better than UC-MSC, at least in the context of diabetic wound healing.http://dx.doi.org/10.1155/2013/592454 |
| spellingShingle | Chandrama Shrestha Liling Zhao Ke Chen Honghui He Zhaohui Mo Enhanced Healing of Diabetic Wounds by Subcutaneous Administration of Human Umbilical Cord Derived Stem Cells and Their Conditioned Media International Journal of Endocrinology |
| title | Enhanced Healing of Diabetic Wounds by Subcutaneous Administration of Human Umbilical Cord Derived Stem Cells and Their Conditioned Media |
| title_full | Enhanced Healing of Diabetic Wounds by Subcutaneous Administration of Human Umbilical Cord Derived Stem Cells and Their Conditioned Media |
| title_fullStr | Enhanced Healing of Diabetic Wounds by Subcutaneous Administration of Human Umbilical Cord Derived Stem Cells and Their Conditioned Media |
| title_full_unstemmed | Enhanced Healing of Diabetic Wounds by Subcutaneous Administration of Human Umbilical Cord Derived Stem Cells and Their Conditioned Media |
| title_short | Enhanced Healing of Diabetic Wounds by Subcutaneous Administration of Human Umbilical Cord Derived Stem Cells and Their Conditioned Media |
| title_sort | enhanced healing of diabetic wounds by subcutaneous administration of human umbilical cord derived stem cells and their conditioned media |
| url | http://dx.doi.org/10.1155/2013/592454 |
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