Intra-Amnionic Threonine Administered to Chicken Embryos Reduces Salmonella Enteritidis Cecal Counts and Improves Posthatch Intestinal Development
This study assessed the effect of in ovo threonine supplementation on the response of broiler chicks challenged with Salmonella Enteritidis, considering bacterial counts in cecal contents, intestinal morphology, body weight, and weight gain. Fertilized eggs were inoculated in the amniotic fluid with...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/9795829 |
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| author | Alexandre L. de B. Moreira Filho Celso J. B. Oliveira Oliveiro C. Freitas Neto Candice M. C. G. de Leon Mauro M. S. Saraiva Maria F. S. Andrade Bryan White Patrícia E. N. Givisiez |
| author_facet | Alexandre L. de B. Moreira Filho Celso J. B. Oliveira Oliveiro C. Freitas Neto Candice M. C. G. de Leon Mauro M. S. Saraiva Maria F. S. Andrade Bryan White Patrícia E. N. Givisiez |
| author_sort | Alexandre L. de B. Moreira Filho |
| collection | DOAJ |
| description | This study assessed the effect of in ovo threonine supplementation on the response of broiler chicks challenged with Salmonella Enteritidis, considering bacterial counts in cecal contents, intestinal morphology, body weight, and weight gain. Fertilized eggs were inoculated in the amniotic fluid with saline (NT) or 3.5% threonine (T) solution at day 17.5 of incubation. At hatch, chicks were individually weighed and cloacal swabs were screened for Salmonella. At 2 days of age, half of the birds from each in ovo treatment were given either 0.5 mL of nutrient broth (sham-inoculated) or nalidixic acid-resistant Salmonella Enteritidis (SE NalR) in nutrient broth (8.3 × 107 colony forming units (CFU) SE NalR/mL). The birds were distributed using a completely randomized design with four treatments after the Salmonella challenge: no in ovo Thr supplementation and sham-inoculated in the posthatch challenge (NT-SHAM), in ovo Thr supplementation and sham-inoculated (T-SHAM), no in ovo Thr supplementation and SE NalR-challenged (NT-SE), and in ovo Thr supplementation and SE NalR-challenged (T-SE). In ovo threonine supplementation reduced Salmonella Enteritidis colonization 168-hour postinoculation and reduced the negative effects associated with Salmonella infection on intestinal morphology and performance, with results similar to those of the sham-inoculated birds. In ovo Thr supplementation increased the expression of MUC2 at hatch and the expression of MUC2 and IgA at 2 days of age and 168-hour postinoculation. Our results suggest that providing in ovo threonine promotes intestinal health in broilers challenged with Salmonella Enteritidis in the first days of life. |
| format | Article |
| id | doaj-art-7032fa5fbf344e97bdda4867204f97f2 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-7032fa5fbf344e97bdda4867204f97f22025-02-03T06:11:47ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/97958299795829Intra-Amnionic Threonine Administered to Chicken Embryos Reduces Salmonella Enteritidis Cecal Counts and Improves Posthatch Intestinal DevelopmentAlexandre L. de B. Moreira Filho0Celso J. B. Oliveira1Oliveiro C. Freitas Neto2Candice M. C. G. de Leon3Mauro M. S. Saraiva4Maria F. S. Andrade5Bryan White6Patrícia E. N. Givisiez7Departmento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba (UFPB), Areia, PB 58397-000, BrazilDepartmento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba (UFPB), Areia, PB 58397-000, BrazilDepartamento de Ciências Veterinárias, Centro de Ciências Agrárias, Universidade Federal da Paraíba (UFPB), Areia, PB 58397-000, BrazilDepartmento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba (UFPB), Areia, PB 58397-000, BrazilDepartmento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba (UFPB), Areia, PB 58397-000, BrazilDepartmento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba (UFPB), Areia, PB 58397-000, BrazilDepartment of Animal Sciences, Division of Nutrition Sciences, University of Illinois at Urbana-Champaign (UIUC), Urbana, IL 61801, USADepartmento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba (UFPB), Areia, PB 58397-000, BrazilThis study assessed the effect of in ovo threonine supplementation on the response of broiler chicks challenged with Salmonella Enteritidis, considering bacterial counts in cecal contents, intestinal morphology, body weight, and weight gain. Fertilized eggs were inoculated in the amniotic fluid with saline (NT) or 3.5% threonine (T) solution at day 17.5 of incubation. At hatch, chicks were individually weighed and cloacal swabs were screened for Salmonella. At 2 days of age, half of the birds from each in ovo treatment were given either 0.5 mL of nutrient broth (sham-inoculated) or nalidixic acid-resistant Salmonella Enteritidis (SE NalR) in nutrient broth (8.3 × 107 colony forming units (CFU) SE NalR/mL). The birds were distributed using a completely randomized design with four treatments after the Salmonella challenge: no in ovo Thr supplementation and sham-inoculated in the posthatch challenge (NT-SHAM), in ovo Thr supplementation and sham-inoculated (T-SHAM), no in ovo Thr supplementation and SE NalR-challenged (NT-SE), and in ovo Thr supplementation and SE NalR-challenged (T-SE). In ovo threonine supplementation reduced Salmonella Enteritidis colonization 168-hour postinoculation and reduced the negative effects associated with Salmonella infection on intestinal morphology and performance, with results similar to those of the sham-inoculated birds. In ovo Thr supplementation increased the expression of MUC2 at hatch and the expression of MUC2 and IgA at 2 days of age and 168-hour postinoculation. Our results suggest that providing in ovo threonine promotes intestinal health in broilers challenged with Salmonella Enteritidis in the first days of life.http://dx.doi.org/10.1155/2018/9795829 |
| spellingShingle | Alexandre L. de B. Moreira Filho Celso J. B. Oliveira Oliveiro C. Freitas Neto Candice M. C. G. de Leon Mauro M. S. Saraiva Maria F. S. Andrade Bryan White Patrícia E. N. Givisiez Intra-Amnionic Threonine Administered to Chicken Embryos Reduces Salmonella Enteritidis Cecal Counts and Improves Posthatch Intestinal Development Journal of Immunology Research |
| title | Intra-Amnionic Threonine Administered to Chicken Embryos Reduces Salmonella Enteritidis Cecal Counts and Improves Posthatch Intestinal Development |
| title_full | Intra-Amnionic Threonine Administered to Chicken Embryos Reduces Salmonella Enteritidis Cecal Counts and Improves Posthatch Intestinal Development |
| title_fullStr | Intra-Amnionic Threonine Administered to Chicken Embryos Reduces Salmonella Enteritidis Cecal Counts and Improves Posthatch Intestinal Development |
| title_full_unstemmed | Intra-Amnionic Threonine Administered to Chicken Embryos Reduces Salmonella Enteritidis Cecal Counts and Improves Posthatch Intestinal Development |
| title_short | Intra-Amnionic Threonine Administered to Chicken Embryos Reduces Salmonella Enteritidis Cecal Counts and Improves Posthatch Intestinal Development |
| title_sort | intra amnionic threonine administered to chicken embryos reduces salmonella enteritidis cecal counts and improves posthatch intestinal development |
| url | http://dx.doi.org/10.1155/2018/9795829 |
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