Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia
Background. In gastric carcinogenesis, changes of DNA methylation appear to be an early molecular event, and the genome-wide methylation state is closely correlated with the level of long interspersed nucleotide element-1 (LINE-1) methylation. In this study, we measured LINE-1 methylation level acco...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2012/360929 |
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| author | Jin Su Kim Woo Chul Chung Kang-Moon Lee Chang Nyol Paik Kyeong Soo Lee Hye Ji Kim Young Wook Kim Ji Han Jung Seung June Noh Yun Kyung Lee |
| author_facet | Jin Su Kim Woo Chul Chung Kang-Moon Lee Chang Nyol Paik Kyeong Soo Lee Hye Ji Kim Young Wook Kim Ji Han Jung Seung June Noh Yun Kyung Lee |
| author_sort | Jin Su Kim |
| collection | DOAJ |
| description | Background. In gastric carcinogenesis, changes of DNA methylation appear to be an early molecular event, and the genome-wide methylation state is closely correlated with the level of long interspersed nucleotide element-1 (LINE-1) methylation. In this study, we measured LINE-1 methylation level according to genetic instability and evaluated the effect of Helicobacter pylori infection on genetic instability in gastric epithelial dysplasia. Methods. Total 100 tissue samples of gastric epithelial dysplasia were analyzed. Seven loci that linked to tumor suppressor genes were used to identify significant structural chromosomal aberrations. Microsatellite status was investigated for two different microsatellite marker loci (BAT25 and BAT26). Also, we measured LINE-1 methylation level by combined bisulfite restriction analysis (COBRA-LINE-1) method. Results. There were no significant differences of LINE-1 methylation level according to chromosomal/microsatellite instability and H. pylori state. In the dysplastic lesions with H. pylori infection, LINE-1 methylation level of MSI lesion was significantly lower than that of microsatellite stable (MSS) lesion (40.23±4.47 versus 43.90±4.81%, P<0.01). Conclusions. In gastric epithelial dysplasia with H. pylori infection, MSI is correlated with reduced LINE-1 methylation level. Coexistence of H. pylori infection and MSI might be a driving force of gastric carcinogenesis. |
| format | Article |
| id | doaj-art-7015d5f4b63b47bdb743f526078d2cee |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-7015d5f4b63b47bdb743f526078d2cee2025-02-03T07:25:57ZengWileyGastroenterology Research and Practice1687-61211687-630X2012-01-01201210.1155/2012/360929360929Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial DysplasiaJin Su Kim0Woo Chul Chung1Kang-Moon Lee2Chang Nyol Paik3Kyeong Soo Lee4Hye Ji Kim5Young Wook Kim6Ji Han Jung7Seung June Noh8Yun Kyung Lee9Department of Internal Medicine, College of Medicine, The Catholic University of Korea, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Republic of KoreaDepartment of Internal Medicine, College of Medicine, The Catholic University of Korea, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Republic of KoreaDepartment of Internal Medicine, College of Medicine, The Catholic University of Korea, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Republic of KoreaDepartment of Internal Medicine, College of Medicine, The Catholic University of Korea, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Republic of KoreaDepartment of Internal Medicine, College of Medicine, The Catholic University of Korea, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Republic of KoreaDepartment of Internal Medicine, College of Medicine, The Catholic University of Korea, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Republic of KoreaDepartment of Internal Medicine, College of Medicine, The Catholic University of Korea, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Republic of KoreaDepartment of Pathology, College of Medicine, The Catholic University of Korea, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Republic of KoreaThe Research Institute of St. Vincent Hospital, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Republic of KoreaDepartment of Pathology, Samsung Medical Center of Korea, 50 Ilwon-dong, Gangnam-gu, Seoul 135-710, Republic of KoreaBackground. In gastric carcinogenesis, changes of DNA methylation appear to be an early molecular event, and the genome-wide methylation state is closely correlated with the level of long interspersed nucleotide element-1 (LINE-1) methylation. In this study, we measured LINE-1 methylation level according to genetic instability and evaluated the effect of Helicobacter pylori infection on genetic instability in gastric epithelial dysplasia. Methods. Total 100 tissue samples of gastric epithelial dysplasia were analyzed. Seven loci that linked to tumor suppressor genes were used to identify significant structural chromosomal aberrations. Microsatellite status was investigated for two different microsatellite marker loci (BAT25 and BAT26). Also, we measured LINE-1 methylation level by combined bisulfite restriction analysis (COBRA-LINE-1) method. Results. There were no significant differences of LINE-1 methylation level according to chromosomal/microsatellite instability and H. pylori state. In the dysplastic lesions with H. pylori infection, LINE-1 methylation level of MSI lesion was significantly lower than that of microsatellite stable (MSS) lesion (40.23±4.47 versus 43.90±4.81%, P<0.01). Conclusions. In gastric epithelial dysplasia with H. pylori infection, MSI is correlated with reduced LINE-1 methylation level. Coexistence of H. pylori infection and MSI might be a driving force of gastric carcinogenesis.http://dx.doi.org/10.1155/2012/360929 |
| spellingShingle | Jin Su Kim Woo Chul Chung Kang-Moon Lee Chang Nyol Paik Kyeong Soo Lee Hye Ji Kim Young Wook Kim Ji Han Jung Seung June Noh Yun Kyung Lee Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia Gastroenterology Research and Practice |
| title | Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia |
| title_full | Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia |
| title_fullStr | Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia |
| title_full_unstemmed | Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia |
| title_short | Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia |
| title_sort | association between genetic instability and helicobacter pylori infection in gastric epithelial dysplasia |
| url | http://dx.doi.org/10.1155/2012/360929 |
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