Mast Cell Interaction with Neutrophils in Human Gastric Carcinomas: Ultrastructural Observations
Aim. The role of mast cells in cell-cell immune interactions has received increasing attention, although their functional interaction with neutrophils still remains to be clarified in tumors. The aim of the present study was to investigate the association between mast cells and neutrophils in a seri...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2016/6891971 |
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| author | Antonio Ieni Valeria Barresi Giovanni Branca Rosario Alberto Caruso Giovanni Tuccari |
| author_facet | Antonio Ieni Valeria Barresi Giovanni Branca Rosario Alberto Caruso Giovanni Tuccari |
| author_sort | Antonio Ieni |
| collection | DOAJ |
| description | Aim. The role of mast cells in cell-cell immune interactions has received increasing attention, although their functional interaction with neutrophils still remains to be clarified in tumors. The aim of the present study was to investigate the association between mast cells and neutrophils in a series of gastric carcinomas (GC). Patients and Methods. 52 surgically resected GC specimens were routinely processed for both light and electron microscopy. Only cases showing both mast cells and neutrophils in the tumor stroma were considered in the analysis. Results. Only 9 GC (M : F = 5 : 4; age range: 50–82 years) showed both mast cells and neutrophils in the tumor stroma. At ultrathin sections, we identified heterotypic aggregation and intermingling of mast cells and neutrophils. Mast cells had mature phenotype and showed full complement of granules with homogeneous, scroll, particle, and mixed pattern. In addition, we found normal-appearing or early apoptosis showing neutrophils. Conclusion. Our histological findings showed the likely interaction between mast cells and neutrophils in GC. We hypothesize that the granular content of mast cells may be released in small quantity through a mechanism called “kiss-and-run fusion,” which is alternative to well-known massive anaphylactic or piecemeal degranulation. |
| format | Article |
| id | doaj-art-700365f256dd47e2a76d22352f8ad982 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | Analytical Cellular Pathology |
| spelling | doaj-art-700365f256dd47e2a76d22352f8ad9822025-02-03T05:52:48ZengWileyAnalytical Cellular Pathology2210-71772210-71852016-01-01201610.1155/2016/68919716891971Mast Cell Interaction with Neutrophils in Human Gastric Carcinomas: Ultrastructural ObservationsAntonio Ieni0Valeria Barresi1Giovanni Branca2Rosario Alberto Caruso3Giovanni Tuccari4Department of Human Pathology “Gaetano Barresi”, University of Messina and Azienda Ospedaliera Universitaria “Policlinico Gaetano Martino”, 98125 Messina, ItalyDepartment of Human Pathology “Gaetano Barresi”, University of Messina and Azienda Ospedaliera Universitaria “Policlinico Gaetano Martino”, 98125 Messina, ItalyDepartment of Human Pathology “Gaetano Barresi”, University of Messina and Azienda Ospedaliera Universitaria “Policlinico Gaetano Martino”, 98125 Messina, ItalyDepartment of Human Pathology “Gaetano Barresi”, University of Messina and Azienda Ospedaliera Universitaria “Policlinico Gaetano Martino”, 98125 Messina, ItalyDepartment of Human Pathology “Gaetano Barresi”, University of Messina and Azienda Ospedaliera Universitaria “Policlinico Gaetano Martino”, 98125 Messina, ItalyAim. The role of mast cells in cell-cell immune interactions has received increasing attention, although their functional interaction with neutrophils still remains to be clarified in tumors. The aim of the present study was to investigate the association between mast cells and neutrophils in a series of gastric carcinomas (GC). Patients and Methods. 52 surgically resected GC specimens were routinely processed for both light and electron microscopy. Only cases showing both mast cells and neutrophils in the tumor stroma were considered in the analysis. Results. Only 9 GC (M : F = 5 : 4; age range: 50–82 years) showed both mast cells and neutrophils in the tumor stroma. At ultrathin sections, we identified heterotypic aggregation and intermingling of mast cells and neutrophils. Mast cells had mature phenotype and showed full complement of granules with homogeneous, scroll, particle, and mixed pattern. In addition, we found normal-appearing or early apoptosis showing neutrophils. Conclusion. Our histological findings showed the likely interaction between mast cells and neutrophils in GC. We hypothesize that the granular content of mast cells may be released in small quantity through a mechanism called “kiss-and-run fusion,” which is alternative to well-known massive anaphylactic or piecemeal degranulation.http://dx.doi.org/10.1155/2016/6891971 |
| spellingShingle | Antonio Ieni Valeria Barresi Giovanni Branca Rosario Alberto Caruso Giovanni Tuccari Mast Cell Interaction with Neutrophils in Human Gastric Carcinomas: Ultrastructural Observations Analytical Cellular Pathology |
| title | Mast Cell Interaction with Neutrophils in Human Gastric Carcinomas: Ultrastructural Observations |
| title_full | Mast Cell Interaction with Neutrophils in Human Gastric Carcinomas: Ultrastructural Observations |
| title_fullStr | Mast Cell Interaction with Neutrophils in Human Gastric Carcinomas: Ultrastructural Observations |
| title_full_unstemmed | Mast Cell Interaction with Neutrophils in Human Gastric Carcinomas: Ultrastructural Observations |
| title_short | Mast Cell Interaction with Neutrophils in Human Gastric Carcinomas: Ultrastructural Observations |
| title_sort | mast cell interaction with neutrophils in human gastric carcinomas ultrastructural observations |
| url | http://dx.doi.org/10.1155/2016/6891971 |
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