Deciphering the Regulatory Network of Some Key Drought-responsive Genes and microRNAs in Canola
Drought is a major abiotic stress that constrains the growth and yield of Canola. This study was conducted to obtain a greater insight into the drought-related hub genes, their regulatory network and relative expression pattern in tolerant and susceptible genotypes of Canola under drought stress. In...
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University of Mazandaran
2024-02-01
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| Series: | Journal of Genetic Resources |
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| Online Access: | https://sc.journals.umz.ac.ir/article_4789_87d389e644ff7f6c7c4cac3eb768042a.pdf |
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| author | Maryam Pasandideh Arjmand Naser Farrokhi Habibollah Samizadeh Lahiji Mohammad Mohsenzadeh Golfazani |
| author_facet | Maryam Pasandideh Arjmand Naser Farrokhi Habibollah Samizadeh Lahiji Mohammad Mohsenzadeh Golfazani |
| author_sort | Maryam Pasandideh Arjmand |
| collection | DOAJ |
| description | Drought is a major abiotic stress that constrains the growth and yield of Canola. This study was conducted to obtain a greater insight into the drought-related hub genes, their regulatory network and relative expression pattern in tolerant and susceptible genotypes of Canola under drought stress. In present study, we sought to find some of key genes and their regulatory network involved in drought stress in Canola, and analyzed gene network, functional pathways, regulatory microRNAs (miRNAs) based on RNA-sequencing data analysis and comparing the relative expression pattern of hub genes in tolerant and susceptible genotypes by Real-time PCR technique. A total of 5275 differentially expressed genes were identified, with 3794 up-regulated and 1481 down-regulated genes under drought stress. The result showed that the most significant biological process of up-regulated and down-regulated genes enriched in response to water deprivation and light stimulus, respectively. The result demonstrated that the ACP4, RCA, FNR1, HCEF1, PRK, GDC, and MDH were some of hub genes in drought stress. The hub genes were regulated by vital drought-responsive miRNAs such as miR9558, miR854, miR172, miR834, miR390, and miR167. The relative expression pattern of investigated hub genes was different in tolerant and susceptible genotypes of Canola. The identified drought-responsive hub genes appear to play an essential role in the regulation of carbon metabolism, activation of stress signaling, and the regulation of the stromal NADP(H) redox state in response to drought stress. They are regulated by important miRNAs in a complex regulatory network that worth being considered in genetic engineering programs of Canola. |
| format | Article |
| id | doaj-art-6fda4b2987004677801302b2008ce1c0 |
| institution | Kabale University |
| issn | 2423-4257 2588-2589 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | University of Mazandaran |
| record_format | Article |
| series | Journal of Genetic Resources |
| spelling | doaj-art-6fda4b2987004677801302b2008ce1c02025-01-27T06:11:19ZengUniversity of MazandaranJournal of Genetic Resources2423-42572588-25892024-02-01101293910.22080/jgr.2024.26535.13804789Deciphering the Regulatory Network of Some Key Drought-responsive Genes and microRNAs in CanolaMaryam Pasandideh Arjmand0Naser Farrokhi1Habibollah Samizadeh Lahiji2Mohammad Mohsenzadeh Golfazani3Department of Plant Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranDepartment of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, IranDepartment of Plant Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranDepartment of Plant Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranDrought is a major abiotic stress that constrains the growth and yield of Canola. This study was conducted to obtain a greater insight into the drought-related hub genes, their regulatory network and relative expression pattern in tolerant and susceptible genotypes of Canola under drought stress. In present study, we sought to find some of key genes and their regulatory network involved in drought stress in Canola, and analyzed gene network, functional pathways, regulatory microRNAs (miRNAs) based on RNA-sequencing data analysis and comparing the relative expression pattern of hub genes in tolerant and susceptible genotypes by Real-time PCR technique. A total of 5275 differentially expressed genes were identified, with 3794 up-regulated and 1481 down-regulated genes under drought stress. The result showed that the most significant biological process of up-regulated and down-regulated genes enriched in response to water deprivation and light stimulus, respectively. The result demonstrated that the ACP4, RCA, FNR1, HCEF1, PRK, GDC, and MDH were some of hub genes in drought stress. The hub genes were regulated by vital drought-responsive miRNAs such as miR9558, miR854, miR172, miR834, miR390, and miR167. The relative expression pattern of investigated hub genes was different in tolerant and susceptible genotypes of Canola. The identified drought-responsive hub genes appear to play an essential role in the regulation of carbon metabolism, activation of stress signaling, and the regulation of the stromal NADP(H) redox state in response to drought stress. They are regulated by important miRNAs in a complex regulatory network that worth being considered in genetic engineering programs of Canola.https://sc.journals.umz.ac.ir/article_4789_87d389e644ff7f6c7c4cac3eb768042a.pdfmirnasppi networkreal-time pcrrna-seq |
| spellingShingle | Maryam Pasandideh Arjmand Naser Farrokhi Habibollah Samizadeh Lahiji Mohammad Mohsenzadeh Golfazani Deciphering the Regulatory Network of Some Key Drought-responsive Genes and microRNAs in Canola Journal of Genetic Resources mirnas ppi network real-time pcr rna-seq |
| title | Deciphering the Regulatory Network of Some Key Drought-responsive Genes and microRNAs in Canola |
| title_full | Deciphering the Regulatory Network of Some Key Drought-responsive Genes and microRNAs in Canola |
| title_fullStr | Deciphering the Regulatory Network of Some Key Drought-responsive Genes and microRNAs in Canola |
| title_full_unstemmed | Deciphering the Regulatory Network of Some Key Drought-responsive Genes and microRNAs in Canola |
| title_short | Deciphering the Regulatory Network of Some Key Drought-responsive Genes and microRNAs in Canola |
| title_sort | deciphering the regulatory network of some key drought responsive genes and micrornas in canola |
| topic | mirnas ppi network real-time pcr rna-seq |
| url | https://sc.journals.umz.ac.ir/article_4789_87d389e644ff7f6c7c4cac3eb768042a.pdf |
| work_keys_str_mv | AT maryampasandideharjmand decipheringtheregulatorynetworkofsomekeydroughtresponsivegenesandmicrornasincanola AT naserfarrokhi decipheringtheregulatorynetworkofsomekeydroughtresponsivegenesandmicrornasincanola AT habibollahsamizadehlahiji decipheringtheregulatorynetworkofsomekeydroughtresponsivegenesandmicrornasincanola AT mohammadmohsenzadehgolfazani decipheringtheregulatorynetworkofsomekeydroughtresponsivegenesandmicrornasincanola |