Peptidylarginine Deiminase 2 in Murine Antiviral and Autoimmune Antibody Responses
The peptidylarginine deiminases (PADs) and the citrullinated proteins that they generate have key roles in innate immunity and rheumatoid arthritis, an inflammatory arthritis with antibodies that target citrullinated proteins. However, the importance of PADs, particularly PAD2, in the adaptive immun...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/5258221 |
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| author | Aisha M. Mergaert Michael F. Denny Brock Kingstad-Bakke Mandar Bawadekar S. Janna Bashar Thomas F. Warner Marulasiddappa Suresh Miriam A. Shelef |
| author_facet | Aisha M. Mergaert Michael F. Denny Brock Kingstad-Bakke Mandar Bawadekar S. Janna Bashar Thomas F. Warner Marulasiddappa Suresh Miriam A. Shelef |
| author_sort | Aisha M. Mergaert |
| collection | DOAJ |
| description | The peptidylarginine deiminases (PADs) and the citrullinated proteins that they generate have key roles in innate immunity and rheumatoid arthritis, an inflammatory arthritis with antibodies that target citrullinated proteins. However, the importance of PADs, particularly PAD2, in the adaptive immune response, both normal and pathogenic, is newly emerging. In this study, we evaluated a requirement for PAD2 in the antibody response in collagen-induced arthritis (CIA), a T and B cell-driven murine model of rheumatoid arthritis, and in the protective antibody response to murine influenza infection. Using PAD2-/- and PAD2+/+ mice on the DBA/1J background, we found that PAD2 is required for maximal anti-collagen antibody levels, but not collagen-specific plasma cell numbers, T cell activation or polarization, or arthritis severity in CIA. Also, we found that PAD2 is required not just for normal levels of persistent hemagglutination inhibiting antibodies but also for full protection from lethal influenza rechallenge. Together, these data provide evidence for a novel modest requirement for PAD2 in a normal antiviral antibody response and in an abnormal autoantibody response in inflammatory arthritis. |
| format | Article |
| id | doaj-art-6fd59b6353344d72996e41b25de3b5fa |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-6fd59b6353344d72996e41b25de3b5fa2025-02-03T06:11:19ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/5258221Peptidylarginine Deiminase 2 in Murine Antiviral and Autoimmune Antibody ResponsesAisha M. Mergaert0Michael F. Denny1Brock Kingstad-Bakke2Mandar Bawadekar3S. Janna Bashar4Thomas F. Warner5Marulasiddappa Suresh6Miriam A. Shelef7Department of MedicineDepartment of MedicineDepartment of Pathobiological SciencesDepartment of MedicineDepartment of MedicineDepartment of Pathology and Laboratory MedicineDepartment of Pathobiological SciencesDepartment of MedicineThe peptidylarginine deiminases (PADs) and the citrullinated proteins that they generate have key roles in innate immunity and rheumatoid arthritis, an inflammatory arthritis with antibodies that target citrullinated proteins. However, the importance of PADs, particularly PAD2, in the adaptive immune response, both normal and pathogenic, is newly emerging. In this study, we evaluated a requirement for PAD2 in the antibody response in collagen-induced arthritis (CIA), a T and B cell-driven murine model of rheumatoid arthritis, and in the protective antibody response to murine influenza infection. Using PAD2-/- and PAD2+/+ mice on the DBA/1J background, we found that PAD2 is required for maximal anti-collagen antibody levels, but not collagen-specific plasma cell numbers, T cell activation or polarization, or arthritis severity in CIA. Also, we found that PAD2 is required not just for normal levels of persistent hemagglutination inhibiting antibodies but also for full protection from lethal influenza rechallenge. Together, these data provide evidence for a novel modest requirement for PAD2 in a normal antiviral antibody response and in an abnormal autoantibody response in inflammatory arthritis.http://dx.doi.org/10.1155/2022/5258221 |
| spellingShingle | Aisha M. Mergaert Michael F. Denny Brock Kingstad-Bakke Mandar Bawadekar S. Janna Bashar Thomas F. Warner Marulasiddappa Suresh Miriam A. Shelef Peptidylarginine Deiminase 2 in Murine Antiviral and Autoimmune Antibody Responses Journal of Immunology Research |
| title | Peptidylarginine Deiminase 2 in Murine Antiviral and Autoimmune Antibody Responses |
| title_full | Peptidylarginine Deiminase 2 in Murine Antiviral and Autoimmune Antibody Responses |
| title_fullStr | Peptidylarginine Deiminase 2 in Murine Antiviral and Autoimmune Antibody Responses |
| title_full_unstemmed | Peptidylarginine Deiminase 2 in Murine Antiviral and Autoimmune Antibody Responses |
| title_short | Peptidylarginine Deiminase 2 in Murine Antiviral and Autoimmune Antibody Responses |
| title_sort | peptidylarginine deiminase 2 in murine antiviral and autoimmune antibody responses |
| url | http://dx.doi.org/10.1155/2022/5258221 |
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