Erythrocyte and Reticulocyte Indices on the LH 750 as Potential Markers of Functional Iron Deficiency
Reticulocyte hemoglobin content (CHr) and percentage of hypochromic cells (%Hypo) are restricted to the Siemens analysers. The aims of the study were to investigate the correlation of Red cells size factor (RSf) and low Hemoglobin density (LHD%), reported by Beckman-Coulter analysers, with CHr and...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | Anemia |
| Online Access: | http://dx.doi.org/10.1155/2010/625919 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558597644484608 |
|---|---|
| author | Eloísa Urrechaga Luís Borque Jesús F. Escanero |
| author_facet | Eloísa Urrechaga Luís Borque Jesús F. Escanero |
| author_sort | Eloísa Urrechaga |
| collection | DOAJ |
| description | Reticulocyte hemoglobin content (CHr) and percentage of hypochromic cells
(%Hypo) are restricted to the Siemens analysers.
The aims of the study were to investigate the correlation of Red cells size factor
(RSf) and low Hemoglobin density (LHD%), reported by Beckman-Coulter
analysers, with CHr and %Hypo in the assessment of iron status in the presence of
inflammation.
381 samples were run on both LH 750 (Beckman-Coulter) and Advia 2120 (Siemens)
analysers. Correlation between parameters were calculated and the diagnostic
performance of the new parameters was assessed.
Results. Correlation between RSf and CHr, 𝑟=0.85. ROC curve analysis for RSf in the diagnosis of iron restricted erythropoiesis defined
as CHr<28 pg: AUC 0.983; Cutoff 91.1%; Sensitivity 98.8%; Specificity 89.6% Correlation between LHD% and %Hypo, 𝑟=0.869.
ROC curve analysis for LHD% in the diagnosis of iron deficiency defined by
%Hypo >5%: AUC 0.954; Cut off 6.0%; Sensitivity 96.6%; Specificity 83.2%
Conclusions. RSf and LHD% could be reliable parameters for the study of iron
metabolism status. |
| format | Article |
| id | doaj-art-6fc3abc0c03f41b0abea34ce5a2db467 |
| institution | Kabale University |
| issn | 2090-1267 2090-1275 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Anemia |
| spelling | doaj-art-6fc3abc0c03f41b0abea34ce5a2db4672025-02-03T01:32:01ZengWileyAnemia2090-12672090-12752010-01-01201010.1155/2010/625919625919Erythrocyte and Reticulocyte Indices on the LH 750 as Potential Markers of Functional Iron DeficiencyEloísa Urrechaga0Luís Borque1Jesús F. Escanero2Hematology Laboratory, Hospital Galdakao—Usansolo, Galdakao, 48960 Vizcaya, SpainDepartment of Pharmacology and Physiology, Faculty of Medicine, University of Zaragoza, Zaragoza, SpainDepartment of Pharmacology and Physiology, Faculty of Medicine, University of Zaragoza, Zaragoza, SpainReticulocyte hemoglobin content (CHr) and percentage of hypochromic cells (%Hypo) are restricted to the Siemens analysers. The aims of the study were to investigate the correlation of Red cells size factor (RSf) and low Hemoglobin density (LHD%), reported by Beckman-Coulter analysers, with CHr and %Hypo in the assessment of iron status in the presence of inflammation. 381 samples were run on both LH 750 (Beckman-Coulter) and Advia 2120 (Siemens) analysers. Correlation between parameters were calculated and the diagnostic performance of the new parameters was assessed. Results. Correlation between RSf and CHr, 𝑟=0.85. ROC curve analysis for RSf in the diagnosis of iron restricted erythropoiesis defined as CHr<28 pg: AUC 0.983; Cutoff 91.1%; Sensitivity 98.8%; Specificity 89.6% Correlation between LHD% and %Hypo, 𝑟=0.869. ROC curve analysis for LHD% in the diagnosis of iron deficiency defined by %Hypo >5%: AUC 0.954; Cut off 6.0%; Sensitivity 96.6%; Specificity 83.2% Conclusions. RSf and LHD% could be reliable parameters for the study of iron metabolism status.http://dx.doi.org/10.1155/2010/625919 |
| spellingShingle | Eloísa Urrechaga Luís Borque Jesús F. Escanero Erythrocyte and Reticulocyte Indices on the LH 750 as Potential Markers of Functional Iron Deficiency Anemia |
| title | Erythrocyte and Reticulocyte Indices on the LH 750 as Potential Markers of Functional Iron Deficiency |
| title_full | Erythrocyte and Reticulocyte Indices on the LH 750 as Potential Markers of Functional Iron Deficiency |
| title_fullStr | Erythrocyte and Reticulocyte Indices on the LH 750 as Potential Markers of Functional Iron Deficiency |
| title_full_unstemmed | Erythrocyte and Reticulocyte Indices on the LH 750 as Potential Markers of Functional Iron Deficiency |
| title_short | Erythrocyte and Reticulocyte Indices on the LH 750 as Potential Markers of Functional Iron Deficiency |
| title_sort | erythrocyte and reticulocyte indices on the lh 750 as potential markers of functional iron deficiency |
| url | http://dx.doi.org/10.1155/2010/625919 |
| work_keys_str_mv | AT eloisaurrechaga erythrocyteandreticulocyteindicesonthelh750aspotentialmarkersoffunctionalirondeficiency AT luisborque erythrocyteandreticulocyteindicesonthelh750aspotentialmarkersoffunctionalirondeficiency AT jesusfescanero erythrocyteandreticulocyteindicesonthelh750aspotentialmarkersoffunctionalirondeficiency |