Erythrocyte and Reticulocyte Indices on the LH 750 as Potential Markers of Functional Iron Deficiency
Reticulocyte hemoglobin content (CHr) and percentage of hypochromic cells (%Hypo) are restricted to the Siemens analysers. The aims of the study were to investigate the correlation of Red cells size factor (RSf) and low Hemoglobin density (LHD%), reported by Beckman-Coulter analysers, with CHr and...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
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| Series: | Anemia |
| Online Access: | http://dx.doi.org/10.1155/2010/625919 |
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| Summary: | Reticulocyte hemoglobin content (CHr) and percentage of hypochromic cells
(%Hypo) are restricted to the Siemens analysers.
The aims of the study were to investigate the correlation of Red cells size factor
(RSf) and low Hemoglobin density (LHD%), reported by Beckman-Coulter
analysers, with CHr and %Hypo in the assessment of iron status in the presence of
inflammation.
381 samples were run on both LH 750 (Beckman-Coulter) and Advia 2120 (Siemens)
analysers. Correlation between parameters were calculated and the diagnostic
performance of the new parameters was assessed.
Results. Correlation between RSf and CHr, 𝑟=0.85. ROC curve analysis for RSf in the diagnosis of iron restricted erythropoiesis defined
as CHr<28 pg: AUC 0.983; Cutoff 91.1%; Sensitivity 98.8%; Specificity 89.6% Correlation between LHD% and %Hypo, 𝑟=0.869.
ROC curve analysis for LHD% in the diagnosis of iron deficiency defined by
%Hypo >5%: AUC 0.954; Cut off 6.0%; Sensitivity 96.6%; Specificity 83.2%
Conclusions. RSf and LHD% could be reliable parameters for the study of iron
metabolism status. |
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| ISSN: | 2090-1267 2090-1275 |