Generation of stably expressing IRF5 spliced isoform in Jurkat cells
Lentiviral transduction enables the generation of gain-of-function of a targeted gene in mammalian cells. Single-cell cloning through limiting dilution can establish a population of cells with homogenous transgene expression for exploring protein function. Here, we describe step by step optimized p...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
HH Publisher
2020-11-01
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| Series: | Progress in Microbes and Molecular Biology |
| Online Access: | https://journals.hh-publisher.com/index.php/pmmb/article/view/337 |
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