Ion‐Permeable Electrospun Scaffolds Enable Controlled In‐Vitro Electrostimulation Assay of Myoblasts
Abstract In‐vitro models are fundamental for studying muscular cell contractility and for wide‐screening of therapeutic candidates targeting skeletal muscle diseases, owing to their scalability, reproducibility, and circumvention of ethical concerns. However, in‐vitro assays permitting reliable elec...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2025-02-01
|
| Series: | Advanced Materials Interfaces |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/admi.202400601 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832542897239490560 |
|---|---|
| author | Serafina Pacilio Francesco Decataldo Roberta Costa Tobias Cramer Beatrice Fraboni Giovanna Cenacchi Maria Letizia Focarete |
| author_facet | Serafina Pacilio Francesco Decataldo Roberta Costa Tobias Cramer Beatrice Fraboni Giovanna Cenacchi Maria Letizia Focarete |
| author_sort | Serafina Pacilio |
| collection | DOAJ |
| description | Abstract In‐vitro models are fundamental for studying muscular cell contractility and for wide‐screening of therapeutic candidates targeting skeletal muscle diseases, owing to their scalability, reproducibility, and circumvention of ethical concerns. However, in‐vitro assays permitting reliable electrical stimulation of cell contractile activity still require technological development. Here, a novel approach to electrically stimulate differentiated muscular cell contractility is reported exploiting the ionic conductivity and mechanical flexibility of 3D nanofibrous scaffolds. The electrospun poly(L‐lactide‐co‐caprolactone) scaffold allowed for C2C12 murine myoblasts horizontal elongation and myotubes formation. Scaffold porosity enables high ionic conductivity and strong electric field generation, orthogonally oriented to the scaffold surface. Electrically induced cell contractility is determined with atomic force microscopy (AFM) enabling real‐time monitoring of scaffold vibrations in liquid environment. Differentiated cell actuation is found to be linearly correlated to current amplitude and number of current stimuli. Integrating the 3D nanofibrous scaffolds with real‐time AFM monitoring provides highly accurate in‐vitro assays for biomedical research. The induction of electric fields orthogonal to the scaffold surface allows for accurately mimicking the excitation‐contraction coupling mechanism observed in native skeletal muscle tissue. This work paves the way for the quantitative study of muscular cell dynamic behavior and physiology, further evaluating therapy effectiveness for muscular pathologies. |
| format | Article |
| id | doaj-art-6fafb34b03d641f7b885ccabcde54543 |
| institution | Kabale University |
| issn | 2196-7350 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj-art-6fafb34b03d641f7b885ccabcde545432025-02-03T13:24:06ZengWiley-VCHAdvanced Materials Interfaces2196-73502025-02-01123n/an/a10.1002/admi.202400601Ion‐Permeable Electrospun Scaffolds Enable Controlled In‐Vitro Electrostimulation Assay of MyoblastsSerafina Pacilio0Francesco Decataldo1Roberta Costa2Tobias Cramer3Beatrice Fraboni4Giovanna Cenacchi5Maria Letizia Focarete6Department of Biomedical and Neuromotor Sciences Alma Mater Studiorum—University of Bologna Via G. Massarenti, 9 Bologna 40138 ItalyDepartment of Medical and Surgical Sciences AlmaMater Studiorum–University of Bologna Via G. Massarenti, 9 Bologna 40138 ItalyDepartment of Biomedical and Neuromotor Sciences Alma Mater Studiorum—University of Bologna Via G. Massarenti, 9 Bologna 40138 ItalyDepartment of Physics and Astronomy Alma Mater Studiorum–University of Bologna Viale Berti Pichat 6/2 Bologna 40127 ItalyDepartment of Physics and Astronomy Alma Mater Studiorum–University of Bologna Viale Berti Pichat 6/2 Bologna 40127 ItalyDepartment of Medical and Surgical Sciences AlmaMater Studiorum–University of Bologna Via G. Massarenti, 9 Bologna 40138 ItalyDepartment of Chemistry “Giacomo Ciamician” and INSTM UdR of Bologna University of Bologna Via Selmi, 2 Bologna 40126 ItalyAbstract In‐vitro models are fundamental for studying muscular cell contractility and for wide‐screening of therapeutic candidates targeting skeletal muscle diseases, owing to their scalability, reproducibility, and circumvention of ethical concerns. However, in‐vitro assays permitting reliable electrical stimulation of cell contractile activity still require technological development. Here, a novel approach to electrically stimulate differentiated muscular cell contractility is reported exploiting the ionic conductivity and mechanical flexibility of 3D nanofibrous scaffolds. The electrospun poly(L‐lactide‐co‐caprolactone) scaffold allowed for C2C12 murine myoblasts horizontal elongation and myotubes formation. Scaffold porosity enables high ionic conductivity and strong electric field generation, orthogonally oriented to the scaffold surface. Electrically induced cell contractility is determined with atomic force microscopy (AFM) enabling real‐time monitoring of scaffold vibrations in liquid environment. Differentiated cell actuation is found to be linearly correlated to current amplitude and number of current stimuli. Integrating the 3D nanofibrous scaffolds with real‐time AFM monitoring provides highly accurate in‐vitro assays for biomedical research. The induction of electric fields orthogonal to the scaffold surface allows for accurately mimicking the excitation‐contraction coupling mechanism observed in native skeletal muscle tissue. This work paves the way for the quantitative study of muscular cell dynamic behavior and physiology, further evaluating therapy effectiveness for muscular pathologies.https://doi.org/10.1002/admi.202400601cell differentiationelectrical stimulationelectrospinningmyoblastsporous scaffolds |
| spellingShingle | Serafina Pacilio Francesco Decataldo Roberta Costa Tobias Cramer Beatrice Fraboni Giovanna Cenacchi Maria Letizia Focarete Ion‐Permeable Electrospun Scaffolds Enable Controlled In‐Vitro Electrostimulation Assay of Myoblasts Advanced Materials Interfaces cell differentiation electrical stimulation electrospinning myoblasts porous scaffolds |
| title | Ion‐Permeable Electrospun Scaffolds Enable Controlled In‐Vitro Electrostimulation Assay of Myoblasts |
| title_full | Ion‐Permeable Electrospun Scaffolds Enable Controlled In‐Vitro Electrostimulation Assay of Myoblasts |
| title_fullStr | Ion‐Permeable Electrospun Scaffolds Enable Controlled In‐Vitro Electrostimulation Assay of Myoblasts |
| title_full_unstemmed | Ion‐Permeable Electrospun Scaffolds Enable Controlled In‐Vitro Electrostimulation Assay of Myoblasts |
| title_short | Ion‐Permeable Electrospun Scaffolds Enable Controlled In‐Vitro Electrostimulation Assay of Myoblasts |
| title_sort | ion permeable electrospun scaffolds enable controlled in vitro electrostimulation assay of myoblasts |
| topic | cell differentiation electrical stimulation electrospinning myoblasts porous scaffolds |
| url | https://doi.org/10.1002/admi.202400601 |
| work_keys_str_mv | AT serafinapacilio ionpermeableelectrospunscaffoldsenablecontrolledinvitroelectrostimulationassayofmyoblasts AT francescodecataldo ionpermeableelectrospunscaffoldsenablecontrolledinvitroelectrostimulationassayofmyoblasts AT robertacosta ionpermeableelectrospunscaffoldsenablecontrolledinvitroelectrostimulationassayofmyoblasts AT tobiascramer ionpermeableelectrospunscaffoldsenablecontrolledinvitroelectrostimulationassayofmyoblasts AT beatricefraboni ionpermeableelectrospunscaffoldsenablecontrolledinvitroelectrostimulationassayofmyoblasts AT giovannacenacchi ionpermeableelectrospunscaffoldsenablecontrolledinvitroelectrostimulationassayofmyoblasts AT marialetiziafocarete ionpermeableelectrospunscaffoldsenablecontrolledinvitroelectrostimulationassayofmyoblasts |