HPTLC determination of diosgenin in fenugreek seeds
A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of...
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| Format: | Article |
| Language: | English |
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Sciendo
2018-03-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2018-0002 |
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| author | Król-Kogus Barbara Lamine Khenifi Mohammed Migas Piotr Boudjeniba Messaoud Krauze-Baranowska Mirosława |
| author_facet | Król-Kogus Barbara Lamine Khenifi Mohammed Migas Piotr Boudjeniba Messaoud Krauze-Baranowska Mirosława |
| author_sort | Król-Kogus Barbara |
| collection | DOAJ |
| description | A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L-1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent. The method was preliminarily validated and the determined amounts of diosgenin in fenugreek seeds of Polish and African origin were found to be similar and ranged from 0.12-0.18 %. |
| format | Article |
| id | doaj-art-6fab08d145784cb39c059d5b8044dc67 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2018-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-6fab08d145784cb39c059d5b8044dc672025-02-02T23:02:22ZengSciendoActa Pharmaceutica1846-95582018-03-016819710710.2478/acph-2018-0002acph-2018-0002HPTLC determination of diosgenin in fenugreek seedsKról-Kogus Barbara0Lamine Khenifi Mohammed1Migas Piotr2Boudjeniba Messaoud3Krauze-Baranowska Mirosława4Department of Pharmacognosy with Medicinal Plants Garden Medical University of Gdańsk 80-416 Gdańsk, PolandLaboratoire d’Ethnobotanique et Substances Naturelles Ecole Normale Supérieure de Kouba B.P 92 Kouba, 16050 AlgerieDepartment of Pharmacognosy with Medicinal Plants Garden Medical University of Gdańsk 80-416 Gdańsk, PolandLaboratoire d’Ethnobotanique et Substances Naturelles Ecole Normale Supérieure de Kouba B.P 92 Kouba, 16050 AlgerieDepartment of Pharmacognosy with Medicinal Plants Garden Medical University of Gdańsk 80-416 Gdańsk, PolandA new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L-1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent. The method was preliminarily validated and the determined amounts of diosgenin in fenugreek seeds of Polish and African origin were found to be similar and ranged from 0.12-0.18 %.https://doi.org/10.2478/acph-2018-0002fenugreek (trigonella foenum-graecum)diosgeninhptlcquantitative analysis |
| spellingShingle | Król-Kogus Barbara Lamine Khenifi Mohammed Migas Piotr Boudjeniba Messaoud Krauze-Baranowska Mirosława HPTLC determination of diosgenin in fenugreek seeds Acta Pharmaceutica fenugreek (trigonella foenum-graecum) diosgenin hptlc quantitative analysis |
| title | HPTLC determination of diosgenin in fenugreek seeds |
| title_full | HPTLC determination of diosgenin in fenugreek seeds |
| title_fullStr | HPTLC determination of diosgenin in fenugreek seeds |
| title_full_unstemmed | HPTLC determination of diosgenin in fenugreek seeds |
| title_short | HPTLC determination of diosgenin in fenugreek seeds |
| title_sort | hptlc determination of diosgenin in fenugreek seeds |
| topic | fenugreek (trigonella foenum-graecum) diosgenin hptlc quantitative analysis |
| url | https://doi.org/10.2478/acph-2018-0002 |
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