Lung Matrix Metalloproteinase Activation following Partial Hepatic Ischemia/Reperfusion Injury in Rats
Purpose. Warm hepatic ischemia-reperfusion (I/R) injury can lead to multiorgan dysfunction. The aim of the present study was to investigate whether acute liver I/R does affect the function and/or structure of remote organs such as lung, kidney, and heart via modulation of extracellular matrix remode...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/867548 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558181963792384 |
|---|---|
| author | Giuseppina Palladini Andrea Ferrigno Vittoria Rizzo Eleonora Tarantola Vittorio Bertone Isabel Freitas Stefano Perlini Plinio Richelmi Mariapia Vairetti |
| author_facet | Giuseppina Palladini Andrea Ferrigno Vittoria Rizzo Eleonora Tarantola Vittorio Bertone Isabel Freitas Stefano Perlini Plinio Richelmi Mariapia Vairetti |
| author_sort | Giuseppina Palladini |
| collection | DOAJ |
| description | Purpose. Warm hepatic ischemia-reperfusion (I/R) injury can lead to multiorgan dysfunction. The aim of the present study was to investigate whether acute liver I/R does affect the function and/or structure of remote organs such as lung, kidney, and heart via modulation of extracellular matrix remodelling. Methods. Male Sprague-Dawley rats were subjected to 30 min partial hepatic ischemia by clamping the hepatic artery and the portal vein. After a 60 min reperfusion, liver, lung, kidney, and heart biopsies and blood samples were collected. Serum hepatic enzymes, creatinine, urea, Troponin I and TNF-alpha, and tissue matrix metalloproteinases (MMP-2, MMP-9), myeloperoxidase (MPO), malondialdehyde (MDA), and morphology were monitored. Results. Serum levels of hepatic enzymes and TNF-alpha were concomitantly increased during hepatic I/R. An increase in hepatic MMP-2 and MMP-9 activities was substantiated by tissue morphology alterations. Notably, acute hepatic I/R affect the lung inasmuch as MMP-9 activity and MPO levels were increased. No difference in MMPs and MPO was observed in kidney and heart. Conclusions. Although the underlying mechanism needs further investigation, this is the first study in which the MMP activation in a distant organ is reported; this event is probably TNF-alpha-mediated and the lung appears as the first remote organ to be involved in hepatic I/R injury. |
| format | Article |
| id | doaj-art-6fa87597326e451eb833a44b7fd991cf |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-6fa87597326e451eb833a44b7fd991cf2025-02-03T01:33:07ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/867548867548Lung Matrix Metalloproteinase Activation following Partial Hepatic Ischemia/Reperfusion Injury in RatsGiuseppina Palladini0Andrea Ferrigno1Vittoria Rizzo2Eleonora Tarantola3Vittorio Bertone4Isabel Freitas5Stefano Perlini6Plinio Richelmi7Mariapia Vairetti8Department of Internal Medicine and Therapeutics, Fondazione IRCCS Policlinico S. Matteo, University of Pavia, Via Ferrata 9A, 27100 Pavia, ItalyDepartment of Internal Medicine and Therapeutics, Fondazione IRCCS Policlinico S. Matteo, University of Pavia, Via Ferrata 9A, 27100 Pavia, ItalyDepartment of Molecular Medicine, Fondazione IRCCS Policlinico S. Matteo, University of Pavia, 27100 Pavia, ItalyDepartment of Biology and Biotechnology “Lazzaro Spallanzani,” University of Pavia, 27100 Pavia, ItalyDepartment of Biology and Biotechnology “Lazzaro Spallanzani,” University of Pavia, 27100 Pavia, ItalyDepartment of Biology and Biotechnology “Lazzaro Spallanzani,” University of Pavia, 27100 Pavia, ItalyDepartment of Internal Medicine and Therapeutics, Fondazione IRCCS Policlinico S. Matteo, University of Pavia, Via Ferrata 9A, 27100 Pavia, ItalyDepartment of Internal Medicine and Therapeutics, Fondazione IRCCS Policlinico S. Matteo, University of Pavia, Via Ferrata 9A, 27100 Pavia, ItalyDepartment of Internal Medicine and Therapeutics, Fondazione IRCCS Policlinico S. Matteo, University of Pavia, Via Ferrata 9A, 27100 Pavia, ItalyPurpose. Warm hepatic ischemia-reperfusion (I/R) injury can lead to multiorgan dysfunction. The aim of the present study was to investigate whether acute liver I/R does affect the function and/or structure of remote organs such as lung, kidney, and heart via modulation of extracellular matrix remodelling. Methods. Male Sprague-Dawley rats were subjected to 30 min partial hepatic ischemia by clamping the hepatic artery and the portal vein. After a 60 min reperfusion, liver, lung, kidney, and heart biopsies and blood samples were collected. Serum hepatic enzymes, creatinine, urea, Troponin I and TNF-alpha, and tissue matrix metalloproteinases (MMP-2, MMP-9), myeloperoxidase (MPO), malondialdehyde (MDA), and morphology were monitored. Results. Serum levels of hepatic enzymes and TNF-alpha were concomitantly increased during hepatic I/R. An increase in hepatic MMP-2 and MMP-9 activities was substantiated by tissue morphology alterations. Notably, acute hepatic I/R affect the lung inasmuch as MMP-9 activity and MPO levels were increased. No difference in MMPs and MPO was observed in kidney and heart. Conclusions. Although the underlying mechanism needs further investigation, this is the first study in which the MMP activation in a distant organ is reported; this event is probably TNF-alpha-mediated and the lung appears as the first remote organ to be involved in hepatic I/R injury.http://dx.doi.org/10.1155/2014/867548 |
| spellingShingle | Giuseppina Palladini Andrea Ferrigno Vittoria Rizzo Eleonora Tarantola Vittorio Bertone Isabel Freitas Stefano Perlini Plinio Richelmi Mariapia Vairetti Lung Matrix Metalloproteinase Activation following Partial Hepatic Ischemia/Reperfusion Injury in Rats The Scientific World Journal |
| title | Lung Matrix Metalloproteinase Activation following Partial Hepatic Ischemia/Reperfusion Injury in Rats |
| title_full | Lung Matrix Metalloproteinase Activation following Partial Hepatic Ischemia/Reperfusion Injury in Rats |
| title_fullStr | Lung Matrix Metalloproteinase Activation following Partial Hepatic Ischemia/Reperfusion Injury in Rats |
| title_full_unstemmed | Lung Matrix Metalloproteinase Activation following Partial Hepatic Ischemia/Reperfusion Injury in Rats |
| title_short | Lung Matrix Metalloproteinase Activation following Partial Hepatic Ischemia/Reperfusion Injury in Rats |
| title_sort | lung matrix metalloproteinase activation following partial hepatic ischemia reperfusion injury in rats |
| url | http://dx.doi.org/10.1155/2014/867548 |
| work_keys_str_mv | AT giuseppinapalladini lungmatrixmetalloproteinaseactivationfollowingpartialhepaticischemiareperfusioninjuryinrats AT andreaferrigno lungmatrixmetalloproteinaseactivationfollowingpartialhepaticischemiareperfusioninjuryinrats AT vittoriarizzo lungmatrixmetalloproteinaseactivationfollowingpartialhepaticischemiareperfusioninjuryinrats AT eleonoratarantola lungmatrixmetalloproteinaseactivationfollowingpartialhepaticischemiareperfusioninjuryinrats AT vittoriobertone lungmatrixmetalloproteinaseactivationfollowingpartialhepaticischemiareperfusioninjuryinrats AT isabelfreitas lungmatrixmetalloproteinaseactivationfollowingpartialhepaticischemiareperfusioninjuryinrats AT stefanoperlini lungmatrixmetalloproteinaseactivationfollowingpartialhepaticischemiareperfusioninjuryinrats AT pliniorichelmi lungmatrixmetalloproteinaseactivationfollowingpartialhepaticischemiareperfusioninjuryinrats AT mariapiavairetti lungmatrixmetalloproteinaseactivationfollowingpartialhepaticischemiareperfusioninjuryinrats |